– Parsnip Itersonilia looking for solutions?

advertisement
Parsnip Itersonilia –
looking for solutions?
BBSRC PhD CASE Studentship with Elsoms Seeds Ltd
Lauren Chappell
Warwick Crop Centre, University of Warwick
Identifying the problem
• Parsnips are a speciality Umbelliferous crop
within the UK.
• Cover area of 3,100ha.
• Economic value of £64M annually.
• Crop losses primarily due to cankers.
How to solve the problem?
• Cankers have been identified as the primary
cause of Parsnip losses.
• Pathogens responsible for causing lesions
identified.
• Carry out high quality research into the
epidemiology of the pathogens.
• Improve breeding using molecular techniques.
Itersonilia
• Three species of Itersonilia:
– Itersonilia pyriformis
– Itersonilia perplexans
– Itersonilia pastinacae
Itersonilia pastinacae
• Seed-borne pathogen
• Foliar Symptoms
– Necrotic lesions on leaves surrounded by a yellow halo.
• Root Symptoms
– Black cankers on shoulder/crown of root.
How do we know Itersonilia’s the problem?
• Identification of the disease in the field.
• Isolation of the pathogen from infected
leaf/root material.
• Pathogens are cultured in the lab.
• Cultures then used to reinfect parsnip
material – Koch’s postulates.
Research
• What we have:
– Parsnip Transcriptome
– Itersonilia Genome
• What we need:
– Phenotypic (observable) data
– Genotypic data
• Combining these data types for QTL mapping
Marker Technology
• Traditional Parsnip breeding methods can
take up to 18yrs.
• Using marker technology can halve this time.
• The first stage is screening Parsnip line for
resistance and susceptibility to cankers:
– Parsnip lines were screened.
– Resistance and susceptibility were assessed.
Resistance Screening Results
lesion size (cm2)
Resistance
9
8
7
6
5
4
3
2
1
0
Susceptibility
Parsnip lines
Future Work
• Further work into the pathogens causing
cankers and lesions
• Further resistance assays on parsnip lines
• Assembly of genetic maps
• Identification of markers linked to QTLs
Acknowledgements
Mrs. Sue Kennedy
Dr. Adrian Dunford
Dr. Richard Tudor
Dr. John Clarkson
Dr. Guy Barker
Dr. Graham Teakle
Download
Related flashcards
RNA

23 Cards

Medical genetics

20 Cards

Create flashcards