Identification & Classification Systems 1-Numerical Taxonomy Numerical taxonomy (also called computer taxonomy, phenetics, or taxometrics) became widely used in the 1960s. Numerical classification schemes use a large number (frequently 100 or more) of unweighted taxonomically useful characteristics. 2-Phylogenetic Classifications: Toward an Understanding of Evolutionary Relationships among Bacteria Phylogenetic classifications are measures of the genetic divergence of different phyla (biologic divisions). Close phylogenetic relatedness of two organisms implies that they share a recent ancestor, The formal ranks used in the taxonomy of bacteria are listed in Table 1. Table 1. Taxonomic Ranks. Formal Rank Example Kingdom Prokaryotae Division Gracilicutes Class Scotobacteria Order Eubacteriales Family Enterobacteriaceae Genus Escherichia Species coli 3-Bergey's Manual of Systematic Bacteriology Bergey's Manual of Systematic Bacteriology. First published in 1923, the Manual is an effort to classify known bacteria and to make this information accessible in the form of a key. A companion volume, Bergey's Manual of Determinative Bacteriology, serves as an aid in the identification of those bacteria that have been described and cultured. Description of the Major Categories & Groups of Bacteria There are two different groups of prokaryotic organisms: eubacteria and archaebacteria. Eubacteria contain the more common bacteria, Archaebacteria do not produce peptidoglycan, a major difference between them and typical eubacteria. They also differ from eubacteria in that they live in extreme environments (eg, high temperature, high salt, or low pH) and carry out unusual metabolic reactions, such as the formation of methane. Eubacteria Lacking Cell Walls These are microorganisms that lack cell walls (commonly called mycoplasmas and comprising the class Mollicutes) and do not synthesize the precursors of peptidoglycan. They are enclosed by a unit membrane, the plasma membrane They resemble the L forms that can be generated from many species of bacteria (notably gram-positive eubacteria); unlike L forms, however, mycoplasmas never revert to the walled state, and there are no antigenic relationships between mycoplasmas and eubacterial L forms.