Product datasheet
Anti-TCR V beta 10 antibody [G101] ab23348
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Overview
Product name
Anti-TCR V beta 10 antibody [G101]
Description
Mouse monoclonal [G101] to TCR V beta 10
Specificity
Approximately 4.2% of unseparated LEW lymph node cells were detected by this clone.
Reactivity is restricted to alpha/beta T cells, of which 6.7% labelled.
Tested applications
Flow Cyt
Species reactivity
Reacts with: Rat
Immunogen
Tissue/ cell preparation (Rat): T cell clone expressing the TCR V beta 10 gene product
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Purity
IgG fraction
Purification notes
ab23348 has been purified by ion exchange chromatography from tissue culture supernatant.
Clonality
Monoclonal
Clone number
G101
Myeloma
P3x63-Ag8.653
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab23348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Flow Cyt
Application notes
Flow Cyt: Use 10µl for 106 cells in 100µl, or 1/50 - 1/100.
Not tested in other applications.
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Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
T cell receptors recognize foreign antigens which have been processed as small peptides and
bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting
cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one
delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and
expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements
produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to
rearrange the beta and alpha loci. This region represents the germline organization of the T cell
receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C
(constant) segments. During T cell development, the beta chain is synthesized by a
recombination event at the DNA level joining a D segment with a J segment; a V segment is then
joined to the D-J gene. The C segment is later joined by splicing at the RNA level.
Recombination of many different V segments with several J segments provides a wide range of
antigen recognition. Additional diversity is attained by junctional diversity, resulting from the
random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments
and one J segment of the beta locus are known to be incapable of encoding a protein and are
considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which
encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities
involving the T-cell receptor beta locus have been associated with T-cell lymphomas.
Cellular localization
Plasma membrane
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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