Product datasheet
Anti-CD16+CD32 antibody [2.4G2] ab210219
Overview
Product name
Anti-CD16+CD32 antibody [2.4G2]
Description
Rat monoclonal [2.4G2] to CD16+CD32
Tested applications
Functional Studies, ICC/IF, IP, Flow Cyt
Species reactivity
Reacts with: Mouse
General notes
Endotoxin Level: Less than or equal to 0.01 EU/μg, as determined by the LaL assay.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Do Not Freeze. Store In the Dark. Store undiluted.
Storage buffer
pH: 7.2
Constituents: 0.87% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate
Purity
Protein A purified
Purification notes
ab210219 was purified from tissue culture supernatant via affinity chromatography. Purity: >90%
pure as determined by SDS-PAGE analysis.
Clonality
Monoclonal
Clone number
2.4G2
Isotype
IgG2b
Applications
Our Abpromise guarantee covers the use of ab210219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Functional Studies
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
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Target
Relevance
In mouse only the transmembrane form of CD16 is reported to exist, which is expressed on
macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes.
CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.
The lymphocyte Fc gamma receptors recognize the Fc portion of IgG, presented either as
immune complexes or as free antibody. The different classes of receptors are distinct because
of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a
wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils,
platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the
placenta. The Fc gamma type III receptors are higher affinity than the type II and are expressed
on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the
same cell and in varying ratios. The receptors are constitutively expressed, although cytokines
and lymphokines can modulate their expression.
Cellular localization
CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also
as a soluble receptor, produced by a proteolytic cleavage.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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