26 research OMAN DAILY Observer TUESDAY, NOVEMBER 10, 2009 Detection of melamine in meat and meat products in Omani markets The determination of melamine concentrations in meat samples was carried out using the Enzyme Linked Immunosorbent Assay (ELISA) at the Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences at Sultan Qaboos University M eat contamination with various chemical and biological residues is one of the major concerns all around the world. Although there is a significant increase in foodborne outbreaks of diseases worldwide, the chemical contamination of meat and meat products is still the most important issue for consumers. Meat and meat products might be contaminated with environmental materials such as pesticides, chemical, biological and natural toxins as a result of processing and packaging. Contamination can also occur through the use of veterinary drugs such as antibiotics, hormones, growth promotents as results of mass production, commercialised feed formulation such as melamine. Melamine is commonly used to produce various products, including plates, plastic resins, and components of paper and paperboard that may come into contact with food. An investigation was carried out by Dr Isam Kadim (pictured), Dr Osman Mahgoub and Rabea al Maqbaly to find out the level of melamine in meat and meat products available in the Omani market. The study revealed that some meat and meat products available in the Omani market are contaminated with melamine. Dr Isam Kadim (Head: Department of Animal and Veterinary Sciences) was interviewed to highlight more details regarding the potential human health hazards associated with consuming meat contaminated with melamine. Dr Kadim addressed the objectives of this investigation and emphasised the seriousness of meat contamination with melamine in Omani markets. These challenges and recommendations can be used to eliminate food contaminants by implementing guidelines and rules. Increased levels of melamine in food beyond the maximum residue limit can affect human health. He stated that the average concentration of melamine in food from approved industrial uses is estimated at less than 0.015 mg/ kg. This level of melamine in food is minute and does not pose a public health concern. Intentional addition of melamine to food, however, does pose a significant risk. The discovery of melamine in pet food, animal feed and protein sources including wheat gluten, rice protein concentrate, and corn gluten created an urgent need for a rapid method for detecting melamine in food. Subsequent to the pet food incident, it was discovered that waste material from manufactures of pet food was contaminated with either melamine alone or in combination with cyanuric acid which had been added to chicken feed. During this time, investigators also learnt that contaminated wheat gluten had been used in manufacturing aquaculture feeds. In China, melamine was added to diluted milk for the manufacture of powdered infant formula. The adulterated infant formula resulted in 50,000 cases of kidney stones, mostly among children under three years of age. The Omani market is open for various types of meat and meat products from all around the world. Currently there is a growing awareness and concern about contaminates and residuals of various harmful materials on human health. Omani people are quite aware of hazards of meat contaminants including those of melamine. Therefore, screening of melamine residues in meat and meat products is very important. This should be carried out through the most sophisticated and highly sensitive methods to determine melamine concentrations in meat and meat products in the Omani market. Fifty-one meat and meat prod- uct samples representing cattle, sheep and poultry, burger, sausages, mortadella, salami and mince meat were randomly collected. The 51 meat samples represented 35 local, regional and international companies. The determination of melamine concentrations in meat samples was carried out using the Enzyme Linked Immunosorbent Assay (ELISA) at the Department of Animal and Veterinary Sciences, College of Agricultural and Marine Sciences at Sultan Qaboos University. The results revealed that chicken from 8 out of 16 poultry companies sampled are selling products contaminated with melamine in the range of 25.6 to 73.0 mg/kg. Those values were much higher than the maximum residue limit, which may cause kidney stones. Fifty per cent of poultry meat tested was found to be contaminated with more than the maximum residue limit of melamine. The high levels of melamine in meat and meat products must be taken seriously as poultry meat consumption has increased by 75-80 per cent among Omanis in recent years. This increase has occurred as a result of better education, an increase in income and low price of chicken compared to other meats. The study also showed that concentrations of melamine in poultry processing such as sausages, burger and mince meat ranged between 0.00 and 35.2 mg/kg. However, melamine concentrates in cattle and sheep meat samples were below the maximum residue limit. These results indicated that some of the poultry companies may have used feeds contaminated with melamine. This incident prompted many countries and regions worldwide, including China, Hong Kong, Australia, New Zealand, the USA, Canada and the European Union, to establish action limits for the presence of melamine and/or melamine and its analogues in foods. The findings of this study confirmed that the public perception and governmental response must be of paramount importance in setting a regulatory framework and determining the rate and direction of the diffusion of the contaminated meat and meat products with melamine or any other contaminates in the Sultanate of Oman. This entails identifying “Minimum Residue Level”, which should be the official procedure for evaluating the safety of the residues of melamine in food products. This can be achieved by establishing laboratories equipped with sophisticated instruments to detect for contaminants in food before it is delivered to the markets to ensure there are no adverse effects on human health. Moreover, the authorised government agencies should establish technical committees to eliminate the spread of contamination in meat and meat products in the Omani market and protect public health. Suppliers of contaminated free meat and meat products in the Omani market need to strengthen routine sampling and develop techniques to monitor potential hazards of contaminated food on human health through liaison between local and international organisations. The need for developing a research culture By Dr Mansour bin Saif al Manthari, Dean, College of Medicine and Health Sciences P eoples and nations take pride in their research achievements represented in their scientific discoveries and in innovations which make their life easier. This status is not attained by chance or through luck, but come as a logical result of continuous and incessant effort in developing a research culture. Since 1970, the Sultanate of Oman has made considerable progress in developing research infrastructures as well as in the Omanisation of research activities through SQU and the Research Council. Recent years have witnessed a continuous increase in budgets allocated to research and in the availability of laboratories. Yet an important issue is whether financial support itself is sufficient to create a research environment in the Sultanate. Clearly, the basic requirement for creating a research environment is a researcher who can use his/her scientific skills as a platform for discovery and for raising hypotheses which are tested for the sake of arriving at solutions and proofs. He should not accept without scrutiny what is merely available and familiar. It is difficult to acquire these research skills at an advanced educational stage by writing MA theses or PhD dissertations; rather it is best acquired at a much earlier stage, which is why schools and parents should play a major role in shaping a research mentality. Thus, emphasising infrastructures alone in creating a research culture may not yield the desired results if it is not accompanied by a serious process of fostering this same culture in the minds of pupils in the early school years. Consequently, I believe that Omanis should become aware of the significance of research values and voluntarily participate in research whenever possible. Schools are the cornerstone of innovation, so teaching should depend more on comprehension and innovation and less on memorisation and mechanical feedback. This is not attained by teaching research as a subject in the curriculum, but by making research a style of teaching, learning, and practice. Simultaneously, evaluation which is only based on memorisation and feedback information should gradually diminish and be replaced by an evaluation based on understanding and innovation. This requires a major effort but it is achievable if we succeed in changing the evaluation paradigm. Research, it must be emphasised, is a lifestyle which is not created in the final years of university studies, but must, like a seed, be planted at a much earlier stage. I call upon those in charge of research in councils and universities, in co-operation with the Ministry of Education, to direct their attention to young people in their formative years and to inculcate in them the mentality of the discoverer and researcher. Infrastructures and budgets can be allocated to form and encourage conducive research environments in schools. Research development is a difficult task, but it can be achieved through the combined efforts of all sectors of society as they work to make it a lifestyle and a national obligation for the sake of preserving the achievements of our blessed Renaissance. New workshops to improve the skills Strategic research is an important part of SQU’s research drive of postgraduate students and faculty S ince theoretical and practical training is a vital requirement for producing quality research in any academic field, and because of the difficulties faced by postgraduate students in improving their research skills, and academic advisers in their supervising of MA theses and PhD dissertations, the Deanship of Postgraduate Studies has introduced workshops to address these problems. These workshops aim to acquaint postgraduate students with the literature of their research topics and to develop analytical and experimental abilities since such skills are not covered in undergraduate programmes. The workshops depend primarily on the expertise of SQU specialists and academics, and are offered free of charge to employees and senior postgraduate students. Participants with complete attendance records will be given certificates at the end of the workshops. Currently, the Deanship of Postgraduate Studies supervises the following workshops: 1. Supervision of postgraduate research This workshop aims to help faculty improve the quality of their postgraduate supervision by enhancing their skills and thus helping them to train their students to work independently. The target audience is the academic supervisors, and it An interview with Saif al Sinani, Director, Department of Research and Innovation Affairs will be conducted in English. 2. Writing and publishing research paper This workshop introduces researchers to the range of skills needed to take a piece of research from the proposal stage to a paper published in an international journal. Special emphasis is placed on designing the paper, dealing with editors and reviewers, and handling ethical issues. The target audience is senior postgraduate students and researchers, and sessions will be conducted in Arabic and English. 3. Developing postgraduate skills This workshop provides basic training in a variety of skills needed to pursue research and publish results. It includes undertaking litera- ture research, using endnotes, writing proposals and theses, using of statistical packages, critical thinking, as well as communication and presentation skills. The target audience is postgraduate students and faculty, and sessions will be conducted in Arabic and English. 4. First aid procedures in laboratories and work locations This workshop aims at raising awareness among those who are working in labs and in the work field of safety procedures for protection against fire and the dangers of chemical dangers. It also instructs participants on how to use first aid. The target audience is postgraduate students and technicians and it will be presented in English. F unded by His Majesty’s Royal Grant, strategic research at SQU is seen as a fundamental cornerstone for activating research of all types, technical and human, as it has offered researchers wider horizons for carrying out their projects with financial support, using modern equipment and above all with the trust of His Majesty. The wisdom and foresight of His Majesty have laid down the University’s scholarly and research foundations. In an interview with Saif bin Said al Sinani, Director of Research and Innovation Affairs at SQU, it was stated that the year 2000 could be considered as a turning point in SQU’s life as it witnessed a visit by His Majesty which inaugurated a new phase in the University’s development, one that will adopt global changes in higher education, and put SQU on the same footing as advanced universities in the world. During his visit, His Majesty emphasised the importance of in-depth research in achieving these goals and launched his annual Royal Grant in support of selected strategic research produced by SQU faculty. Since 2001, 43 research projects have been financed in the fields of environmental sciences, technology, petroleum, health, engineering and human sciences. These projects dealt with important topics and reflected international standards of procedure. Selection mechanism Selecting strategic research projects follows the same mechanism used in selecting research funded by the University. Researchers from various colleges (Medicine, Engineering, Agriculture and Environment, Education, Sciences and Arts) submit proposals which will support the Sultanate’s development process. These proposals are sent to inhouse as well as international referees at prestigious universities and they are further evaluated by relevant institutions, ministries and corporations. All reports are collected and presented to a committee of specialists which selects the best proposals and recommends funding. Al Sinani explained that submitting proposals is open to the general public on condition that the main researcher is a faculty member at SQU. Associate researchers can be drawn from the local community, represented by the private and government sectors or from prestigious international universities and research centres, depending on the project type. For example a proposal that deals with medical field can include specialists from the Ministry of Health and so on. Al Sinani pointed out that funded projects have achieved tangible and positive results in accordance with their goals and objectives, and that their results will be distributed to all parties concerned. The ceiling for the funding of research is limited neither by the number of projects nor by the funds available. Each project is evaluated on its own merits and allotted an appropriate budget in order to enable the researchers to achieve their goals. The number of research projects submitted annually is not limited as it depends on their quality, their strategic importance to the development of the Sultanate, and their cost within the annual budget allotted for a particular year. Thus, as many as ten, or less projects can be accepted in one year. Al Sinani added that the following five strategic research projects submitted by SQU faculty have been approved this year as they are developmentally relevant: 1. The History of Omani-East African Relations between 1624-1963: A critical and Analytical Study (Faculty of Arts and Social Sciences) 2. Establishment of a National Facility in Stem Cell Translational Research for Novel Cellular-based Therapies and Tissue Repair (College of Medicine) 3. Nosocomial Transmission of Multi-drug Resistant Acinetobacter Strains — the Usefulness of Patient Screening, Assessment of Environmental, Aerial Bacterial Contamination and Various Genotypic and Phenotypic Typing Methodologies (College of Medicine) 4. The Feasibility of Managed Aquifer Recharge Using Excess Treated Wastewater in Oman (College of Agriculture and Marine Sciences) 5. Developing Microfluidic Systems for Routine Analysis of Pharmaceutical Samples (College of Science) Ongoing efforts Al Sinani concluded by saying that SQU’s efforts at developing research will continue throughout the coming years in order to improve the quality of research and encourage academics to produce their best work by providing them with all kinds of facilities conducive for research excellence and innovation.