Product datasheet
Anti-DUOX2 antibody ab170308
2 Images
Overview
Product name
Anti-DUOX2 antibody
Description
Rabbit polyclonal to DUOX2
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide within Human DUOX2 aa 520-550 (internal sequence) conjugated to Keyhole
Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9NRD8
Positive control
CEM cell lysate; CEM cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Purification notes
ab170308 is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab170308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/100 - 1/500. Predicted molecular weight: 175 kDa.
Flow Cyt
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype
control with this antibody.
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Target
Function
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and
lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated
antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its
N-terminal peroxidase-like domain.
Tissue specificity
Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein
level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary
glands, rectum and testis.
Pathway
Hormone biosynthesis; thyroid hormone biosynthesis.
Involvement in disease
Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A
disorder due to a defective conversion of accumulated iodide to organically bound iodine. The
iodide organification defect can be partial or complete.
Sequence similarities
In the N-terminal section; belongs to the peroxidase family.
Contains 3 EF-hand domains.
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
Developmental stage
Widely expressed in fetal tissues.
Post-translational
modifications
N-glycosylated.
Cellular localization
Apical cell membrane. Localizes to the apical membrane of epithelial cells.
Anti-DUOX2 antibody images
Anti-DUOX2 antibody (ab170308) at 1/100
dilution + CEM cell lysate at 35 µg
Predicted band size : 175 kDa
Western blot - Anti-DUOX2 antibody (ab170308)
Flow cytometric analysis of CEM cells
labeling DUOX2 with ab170308 at 1/10
dilution (right histogram), compared
to negative control cells (left histogram). FITCconjugated goat-anti-rabbit secondary
antibodies was used for the analysis.
Flow Cytometry - Anti-DUOX2 antibody
(ab170308)
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