Product datasheet Anti-Apg10 antibody [EPR4804] ab124711 2 Images Overview Product name Anti-Apg10 antibody [EPR4804] Description Rabbit monoclonal [EPR4804] to Apg10 Tested applications WB, IP Species reactivity Reacts with: Human Immunogen Synthetic peptide corresponding to residues on the C-terminus of the Human Apg10 protein (Q9H040). Positive control HeLa, K562, and 293T cell lysates. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Properties Form Liquid Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. Dissociation constant (KD) KD = 9.20 x 10 -11 M Learn more about KD Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR4804 Isotype IgG 1 Applications Our Abpromise guarantee covers the use of ab124711 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa. IP 1/10 - 1/100. Application notes Is unsuitable for Flow Cyt,ICC/IF or IHC-P. Target Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Sequence similarities Belongs to the ATG10 family. Cellular localization Cytoplasm. Anti-Apg10 antibody [EPR4804] images All lanes : Anti-Apg10 antibody [EPR4804] (ab124711) at 1/1000 dilution Lane 1 : HeLa cell lysates Lane 2 : K562 cell lysates Lane 3 : 293T cell lysates Lysates/proteins at 10 µg per lane. Predicted band size : 25 kDa Western blot - Anti-Apg10 antibody [EPR4804] (ab124711) Equilibrium disassociation constant (KD) Learn more about KD Click here to learn more about KD Other-Anti-Apg10 antibody [EPR4804](ab124711) 2 Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3