Product datasheet
Anti-Apg10 antibody [EPR4804] ab124711
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Overview
Product name
Anti-Apg10 antibody [EPR4804]
Description
Rabbit monoclonal [EPR4804] to Apg10
Tested applications
WB, IP
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of the Human Apg10 protein
(Q9H040).
Positive control
HeLa, K562, and 293T cell lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 9.20 x 10 -11 M
Learn more about KD
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4804
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab124711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
Function
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation
of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an
ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3.
Sequence similarities
Belongs to the ATG10 family.
Cellular localization
Cytoplasm.
Anti-Apg10 antibody [EPR4804] images
All lanes : Anti-Apg10 antibody [EPR4804]
(ab124711) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : K562 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size : 25 kDa
Western blot - Anti-Apg10 antibody [EPR4804]
(ab124711)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-Apg10 antibody [EPR4804](ab124711)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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