Product datasheet
Anti-DEGS1 antibody [EPR9681] ab167169
2 Images
Overview
Product name
Anti-DEGS1 antibody [EPR9681]
Description
Rabbit monoclonal [EPR9681] to DEGS1
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide, corresponding to residues near the C terminal of HumanDEGS1 (UniProt:
O15121).
Positive control
293T, T47D and HepG2 cell lysates; Human breast carcinoma tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR9681
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab167169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with
IHC staining protocol.
ICC/IF
Application notes
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Target
Relevance
DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for
inserting double bonds into specific positions in fatty acids. It contains three His containing
consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has
sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythrosphingosine (E-sphing-4-enine).
Cellular localization
Endoplasmic reticulum membrane; Multi pass membrane protein.
Anti-DEGS1 antibody [EPR9681] images
All lanes : Anti-DEGS1 antibody [EPR9681]
(ab167169) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : T47D cell lysates
Lane 3 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Western blot - Anti-DEGS1 antibody [EPR9681]
(ab167169)
Predicted band size : 38 kDa
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Immunohistochemical analysis of paraffinembedded human breast carcinoma tissue
labeling DEGS1 with ab167169 at 1/50
dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-DEGS1
antibody [EPR9681] (ab167169)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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