Product datasheet
Anti-ROCK1 antibody [EPR638Y] ab134181
3 Abreviews 1 References 3 Images
Overview
Product name
Anti-ROCK1 antibody [EPR638Y]
Description
Rabbit monoclonal [EPR638Y] to ROCK1
Tested applications
ICC/IF, IHC-Fr, WB, IP, ICC, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the C-terminus of Human ROCK1.
Positive control
HeLa cell lysate; Human lymphoma tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR638Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab134181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. PubMed: 24465504
1
Application
Abreviews
WB
Notes
1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular
weight: 158 kDa).
IP
1/60.
ICC
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with
IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.
Target
Function
Phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase
through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell
migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth.
Required for centromere positioning and centromere-dependent exit from mitosis. Necessary for
apoptotic membrane blebbing.
Tissue specificity
Detected in blood platelets.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
Domain
The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a
conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
Post-translational
modifications
Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage,
probably by ATM or ATR.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and
membrane blebbing.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus membrane.
Associated with the mother centriole and an intercentriolar linker (By similarity). A small
proportion is associated with Golgi membranes.
Anti-ROCK1 antibody [EPR638Y] images
2
Anti-ROCK1 antibody [EPR638Y]
(ab134181) at 1/500 dilution + HeLa cell
lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000
dilution
Western blot - Anti-ROCK1 antibody [EPR638Y]
(ab134181)
Predicted band size : 158 kDa
Immunohistochemical analysis of paraffin
embedded Human lymphoma tissue labelling
ROCK1 with ab134181 at a dilution of 1/50.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ROCK1
antibody [EPR638Y] (ab134181)
ab134181 (1/200) staining ROCK1 in Hela
cells (green). Cells were fixed in
paraformaldehyde, permeabilised in 0.5%
Triton X100/PBS and counterstained with
DAPI in order to highlight the nucleus (red).
Immunocytochemistry/ Immunofluorescence -
For further experimental details please refer
Anti-ROCK1 antibody [EPR638Y] (ab134181)
to Abreview.
Image courtesy of an Abreview submitted by Dr. Kirk
McManus, Univ. of Manitoba/Cancer Care MICB,
Canada
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
3
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4