Product datasheet
Anti-PI 3 Kinase p85 beta antibody ab62901
3 Images
Overview
Product name
Anti-PI 3 Kinase p85 beta antibody
Description
Rabbit polyclonal to PI 3 Kinase p85 beta
Tested applications
WB, ELISA, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
A KLH conjugated synthetic peptide selected from the N-terminal region of human PI 3 Kinase
p85 beta.
Positive control
WB: 293 cell lysates transiently transfected with the PI 3 Kinase p85 beta gene. IHC-P: Human
brain tissue. ICC/IF: methanol fixed HepG2 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Purity
Protein A purified
Purification notes
ab62901 is purified through a protein A column, eluted with high and low pH buffers and
neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab62901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/100 - 1/500. Detects a band of approximately 82 kDa (predicted molecular
weight: 82 kDa).
ELISA
1/1000.
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Application
Abreviews
Notes
IHC-P
1/50 - 1/100.
ICC/IF
Use a concentration of 5 µg/ml.
Target
Function
Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts
as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Sequence similarities
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated. Dephosphorylated by PTPRJ.
Anti-PI 3 Kinase p85 beta antibody images
ICC/IF image of ab62901 stained HepG2
cells. The cells were 100% methanol fixed (5
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab62901 at 5µg/ml
overnight at +4°C. The secondary antibody
(green) was DyLight® 488 goat anti- rabbit
(ab96899) IgG (H+L) used at a 1/250 dilution
Immunocytochemistry/ Immunofluorescence -
for 1h. Alexa Fluor® 594 WGA was used to
Anti-PI 3 Kinase p85 beta antibody (ab62901)
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
ab62901 at 1/50 - 1/100 dilution, staining PI 3
Kinase p85 beta in human brain by
Immunohistochemistry using formalin-fixed,
paraffin-embedded tissue. ab62901 was
peroxidase-conjugated to the secondary
antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - PI 3 Kinase p85
beta antibody (ab62901)
2
All lanes : Anti-PI 3 Kinase p85 beta
antibody (ab62901) at 1/100 dilution
Lane 1 : 293 cell lysates, nontransfected
Lane 2 : 293 cell lysates, transiently
transfected with the PI 3 Kinase p85 beta
gene
Western blot - PI 3 Kinase p85 beta antibody
Lysates/proteins at 2 µg per lane.
(ab62901)
Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 60 kDa. We are
unsure as to the identity of these extra bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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