Microbiology Lab (BIOL 2230) – Fall 2013 PREP SHEETS
WK
9
Topics
Biochemical Testing III
Orgs
Per Table (Biochem Testing):
11/06
Selective and/or Differential Media
 Mannitol Salt Agar (MSA) 4-1
 S. aureus
 S. epidermidis
 E. coli/E. aerogenes
 M. luteus
 MacConkey Agar (MAC) 4-6
 E. coli/E. aerogenes
 E. faecalis
 S. flexneri
 S. typhimurium
 Blood Agar Plate (BAP) 5-20
 (see demonstration plates)
Disk Tests
 Bacitracin (A) Susceptibility 5-9
 S. pyogenes
 S. agalactiae
 Novobiocin Susceptibility (p. 468)
 S. aureus or S. epidermidis
 S. saprophyticus
 Optochin (P) Susceptibility (p.
468)
 S. bovis
 S. pneumoniae
TSA Slant:
M. luteus
NA Slant:







S. aureus
S. epidermidis
E. coli
E. faecalis
S. typhimurium
S. flexneri
E. aerogenes
Nutrient broths:



S. aureus
S. epidermidis
S. saprophyticus
Media
Per Table:
3 BAP (disk tests)
1 MAC
1 MSA
1 NAP (growth control for selective
Equipment
Per Table:
Tubs
1 package sterile toothpicks
1 beaker w/biohazard bag (used toothpicks)
media)
Bacitracin (A disks)
Optochin (B disks)
Novobiocin disks
12% FeCl2
H2O2
Kovac’s (for SIM from prev. week)
Oxidase reagent
Candle jar
Students will spot inoculate one quadrant
of MAC, MSA and NAP with assigned
organism.
For Class Cultures :
1 TSA x 6 tables
7 NAS x 6 tables
3 NB x 6 tables
5 TGB x 6 tables
(Students will share the 9 broth tubes
between each pair of tables)
TG broths:





S. pyogenes
S. agalactiae
S. peumoniae
S. bovis
E. faecalis
Shannon makes UNKNOWNS!!!
Media Needed:
24 nutrient broth tubes
24 thioglycollate broth tubes
24 sterile tubes
Instructor Cultures:
Hemolysis Demonstration
o
1 BAP w S. aureus
o
1 BAP w S. pyogenes
o
1 BAP w S. agalactiae
o
1 BAP w S. pneumonia or
bovis
Note: All in a candle jar!
Per Student and also on Shannon’s Table:
Sterile swabs
Beakers w/alcohol and forceps
Beakers with EtOH
Sterile tube
Sterile swabs
On Side Bench:
large beaker w/10% bleach (slide disposal)
autoclave tray (culture disposal)