Product datasheet
Anti-Protein Kinase A regulatory subunit I alpha
antibody [6C7] ab118061
9 Images
Overview
Product name
Anti-Protein Kinase A regulatory subunit I alpha antibody [6C7]
Description
Mouse monoclonal [6C7] to Protein Kinase A regulatory subunit I alpha
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Recombinant Human Protein Kinase A regulatory subunit I alpha protein expressed in 293T
cells.
Positive control
HEK293T cell lysate transfected with pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha
cDNA; HeLa, HT29, A549 and Jurkat cell extracts; Human breast adenocarcinoma, bladder
carcinoma, colon, endometrium, ovary and prostate tissues; COS7 cells transiently transfected
by pCMV6-ENTRY Protein Kinase A regulatory subunit I alpha.
General notes
Stable for 12 months from date of receipt.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA
Purity
IgG fraction
Purification notes
ab118061 is purified from Mouse ascites fluids by affinity chromatography.
Clonality
Monoclonal
Clone number
6C7
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab118061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/2000. Predicted molecular weight: 43 kDa.
IHC-P
1/50.
ICC/IF
1/100.
Target
Tissue specificity
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression
varies among tissues and is in some cases constitutive and in others inducible.
Involvement in disease
Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1) [MIM:160980]. CNC is a
multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other
myxomas, endocrine tumors, and psammomatous melanotic schwannomas.
Defects in PRKAR1A are the cause of intracardiac myxoma (INTMYX) [MIM:255960].
Inheritance is autosomal recessive.
Defects in PRKAR1A are the cause of primary pigmented nodular adrenocortical disease type 1
(PPNAD1) [MIM:610489]. Primary pigmented nodular adrenocortical disease is a rare bilateral
adrenal defect causing ACTH-independent Cushing syndrome. Macroscopic appearance of the
adrenals is characteristic with small pigmented micronodules observed in the cortex. PPNAD1
is most often diagnosed in patients with Carney complex, but it can also be observed in patients
without other manifestations or familial history.
Sequence similarities
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
Post-translational
modifications
The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain,
resulting in the inhibition of its activity.
Anti-Protein Kinase A regulatory subunit I alpha antibody [6C7] images
All lanes : Anti-Protein Kinase A regulatory
subunit I alpha antibody [6C7] (ab118061) at
1/2000 dilution
Lane 1 : HEK293T cell lysate, transfected
with the pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected
with pCMV6-ENTRY Protein Kinase A
Western blot - Anti-Protein Kinase A regulatory
regulatory subunit I alpha cDNA
subunit I alpha antibody [6C7] (ab118061)
Lysates/proteins at 5 µg per lane.
Predicted band size : 43 kDa
HEK293T cell lysates were generated from
transient transfection of the cDNA clone
(RC201638)
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All lanes : Anti-Protein Kinase A regulatory
subunit I alpha antibody [6C7] (ab118061) at
1/2000 dilution
Lane 1 : HEK293 cell extract
Lane 2 : HeLa cell extract
Lane 3 : HT29 cell extract
Lane 4 : A549 cell extract
Western blot - Anti-Protein Kinase A regulatory
subunit I alpha antibody [6C7] (ab118061)
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size : 43 kDa
HEK293T cell lysates were generated from
transient transfection of the cDNA clone
(RC201601)
ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Breast
adenocarcinoma tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Colon tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
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ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Ovary tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Endometrium
tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Prostate tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
ab118061 at 1/50 dilution staining Protein
Kinase A regulatory subunit I alpha in
Paraffin-embedded Human Bladder
carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Protein Kinase
A regulatory subunit I alpha antibody [6C7]
(ab118061)
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ab118061 at 1/100 dilution staining Protein
Kinase A regulatory subunit I alpha in COS7
cells transiently transfected by pCMV6ENTRY Protein Kinase A regulatory subunit I
alpha by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence Anti-Protein Kinase A regulatory subunit I alpha
antibody [6C7] (ab118061)
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Replacement or refund for products not performing as stated on the datasheet
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