Product datasheet
Anti-Creatine kinase B type antibody [893CT29.1.1]
ab180040
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Overview
Product name
Anti-Creatine kinase B type antibody [893CT29.1.1]
Description
Mouse monoclonal [893CT29.1.1] to Creatine kinase B type
Tested applications
WB
Species reactivity
Reacts with: Mouse, Human
Immunogen
Recombinant full length protein (His-tag) corresponding to Creatine kinase B type aa 1-381.
Database link: P12277
Positive control
MDA-MB453; 293 and Y-79 cell lysates; Mouse stomach and Mouse brain tissue lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Protein G purified
Purification notes
ab180040 is purified through a protein G column, eluted with high and low pH buffers and
neutralized immediately, followed by dialysis against PBS.
Clonality
Monoclonal
Clone number
893CT29.1.1
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab180040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000. Predicted molecular weight: 42 kDa.
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Target
Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g.
creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in
tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and
spermatozoa.
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
Cellular localization
Cytoplasm.
Anti-Creatine kinase B type antibody [893CT29.1.1] images
All lanes : Anti-Creatine kinase B type
antibody [893CT29.1.1] (ab180040) at
1/1000 dilution
Lane 1 : MDA-MB453 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Y-79 cell lysate
Lane 4 : Mouse stomach tissue lysate
Western blot - Anti-Creatine kinase B type
Lane 5 : Mouse brain tissue lysate
[893CT29.1.1] antibody (ab180040)
Lysates/proteins at 35 µg per lane.
Predicted band size : 42 kDa
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Terms and conditions
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