• Data: Trt (1) a b ab c ac bc abc Block Total Block I 1 2 4 6 4 3 6 10 36 Block II 3 1 5 8 7 3 6 11 44 Trt. Total 4 3 9 14 11 6 12 21 80 2 Stat 402 (Spring 2016): Slide Set 12 A 2 Factorial in a RCBD (Cont’d 1) 3 Last update: March 28, 2016 Stat402 (Spring 2016) Slide set 12 • • • • Stat 402 (Spring 2016): Slide Set 12 ab c (1) bc c b b (1) bc abc a ab ac a abc ac = = = = = Total SS Total (Corrected) SS Block SS Trt SS Error SS ANOVA Table: = CF Computations: = 400 (SS for the Mean) SV Block Trt Error Total DF 1 7 7 15 SS 4 122 6 132 6 (By Subtraction) 362 442 8 + 8 − CF = 404 − 400 = 4 42 32 212 2 + 2 + · · · + 2 − CF = 522 − 532-400=132 1 + 22 + · · · + 62 + 112 = 532 802 16 2 400 = 122 3 Stat 402 (Spring 2016): Slide Set 12 A 2 Factorial in a RCBD (ANOVA) 3 1 • The above table shows an example how the treatment combination were randomly applied to experimental units/runs within each block. Block 1 Block 2 Consider 2 blocks: Treatments: A, B, C each at 2 levels; 8 treatment combinations. Thus we need blocks of size: 8. A 2 Factorial in a RCBD 3 Stat 402 (Spring 2016): Slide Set 12 Observed Total 4 3 9 14 11 6 12 21 I + + + + + + + + 80 16 5.0 16 400 A + + + + 8 8 1.0 16 4 B + + + + 32 8 4.0 16 64 Block I (1) ab Block II a b 6 We shall consider each of these designs separately: • Design 1: Suppose that we have a 22 factorial experiment but we can obtain blocks of only 2 homogeneous units each. We have 4 treatment combinations (1), a, b and ab, which can be allocated to the 2 blocks in 3 different ways to obtain 3 designs. • • Because variation between experimental units within blocks tend to increase as the block size increase (which results in a larger experimental error), it is desirable to keep the block size small. • = = = = μ(1) + β1 + (1) μab + β1 + ab μa + β2 + a μb + β2 + b 7 Here β1 and β2 represent the respective effects of the two blocks. Using this model, we shall look at the contrasts of the treatment combinations which define the main effects and interactions to see the effect of putting 1/2 of the treatment combinations in one block and the other 1/2 in the other block: y(1) yab ya yb We can write the model for the design, assuming one replication of the treatment combinations as follows: A 2 Factorial in Blocks of Size 2 (Cont’d design1 ) Stat 402 (Spring 2016): Slide Set 12 For example, a 27 has 128 different treatment combinations and it is very difficult if not impossible to find blocks of 128 experimental units that are homogenous. (Why?) • 2 In factorial experiments the number of treatment combinations increases rapidly as the number of factors and/or the number of levels of each factor increases. • Stat 402 (Spring 2016): Slide Set 12 ABC + + + + 8 8 1 16 4 5 BC + + + + 0 8 0 16 0 Stat 402 (Spring 2016): Slide Set 12 A 2 Factorial in Incomplete Block Experiments k 4 Factorial Effect AB C AC + + + + + + + + + + + + 20 20 0 8 8 8 2.5 2.5 0 16 16 16 25 25 0 A 2 Factorial in Blocks of Size 2 2 Treatment Combination (1) a b ab c ac bc abc Contrast Divisor for Estimate Estimate of Effect Divisor for SS SS of Effect Now the 7 d.f. for Treatment Sum of Squares is partitioned into 7 single degree of d.f. sums of squares corresponding to the 7 factorial effects: Factorial Effects and their Sums of Squares: • • Stat 402 (Spring 2016): Slide Set 12 Block I (1) b y(1) yb ya yab = = = = Block II a ab μ(1) + β1 + (1) μb + β1 + b μa + β2 + a μab + β2 + ab In this case, the model is given by Design 2: 10 • = 1/2[μab + β2 + ab + μ(1) + β1 + (1) − μa − β2 −a − μb − β1 − b] = 1/2[μab − μa − μb + μ(1)] + 1/2[ab − a − b + (1)] AB 11 If we use this design, then we see that the B effect and the AB interaction effect will be free of block effect and that the main effect A will be confounded with blocks. = 1/2[μab + β2 + ab + μb + β1 + b − μa − β2 −a − μ(1) − β1 − (1)] = 1/2[μab + μb − μa − μ(1)] + 1/2[ab + b − a − (1)] B̂ Â = 1/2[μab + β2 + ab + μa + β2 + a − μb − β1 −b − μ(1) − β1 − (1)] = 1/2[μab + μa − μb − μ(1)] + (β2 − β1) + 1/2[ab + a − b − (1)] Stat 402 (Spring 2016): Slide Set 12 Hence, Stat 402 (Spring 2016): Slide Set 12 From above analysis we can see that, if we use Design 1, the effects of A and B are free of the block effect while the effect of AB is not. • 9 We can see that if we use this design, then we cannot separate the AB interaction effect from the block effect. The effect of AB interaction is said to be confounded with the block effect. = 1/2[ab − a − b + (1)] = 1/2[μab + β1 + ab + μ(1) + β1 + (1) − μa − β2 −a − μb − β2 − b] = 1/2[μab − μa − μb + μ(1)] + (β1 − β2) + 1/2[ab − a − b + (1)] Stat 402 (Spring 2016): Slide Set 12 • AB AB: (a-1)(b-1): 8 = 1/2[ab + b − a − (1)] = 1/2[μab + β1 + ab + μb + β2 + b − μa − β2 −a − μ(1) − β1 − (1)] = 1/2[μab + μb − μa − μ(1)] + 1/2[ab + b − a − (1)] A 2 Factorial in Blocks of Size 2 (Cont’d design 2) 2 B̂ B: (a+1)(b-1) Â = 1/2[ab + a − b − (1)] = 1/2[μab + β1 + ab + μa + β2 + a − μb − β2 −b − μ(1) − β1 − (1)] = 1/2[μab + μa − μb − μ(1)] + 1/2[ab + a − b − (1)] A: (a-1)(b+1) A 2 Factorial in Blocks of Size 2 (Cont’d design 1) 2 = = = = Block II a ab μ(1) + β1 + (1) μa + β1 + a μb + β2 + b μab + β2 + ab Block I (1) a Stat 402 (Spring 2016): Slide Set 12 14 2. Then treatment combinations are randomly applied to the units within each block. 1. Given an experimental plan, numbers are assigned to blocks at random. Randomization in Incomplete Blocks 3. We usually try to confound the higher order interaction effects in a given factorial experiment since these are the least important. When an effect is confound it cannot be estimated; hence, no sum of squares exists to test its effect. 4. Each of the above designs is an example of an incomplete block design (as opposed to a complete block design) 5. Confounding can be thought of as a method available for increasing the accuracy of factorial experiments by reducing the block size at the expense of reduced accuracy on estimating some of the effects. y(1) ya yb yab The model in this case is Design 3: 12 Stat 402 (Spring 2016): Slide Set 12 A 2 Factorial in Blocks of Size 2 (Cont’d design 3) 2 2. It is possible to confound a given effect by the choice of the design. 1. Only one effect is confounded with blocks in each design 13 In this design, A and AB effects are free of block effects and the main effect B is confounded with block effect. Remarks • Stat 402 (Spring 2016): Slide Set 12 Â = 1/2[μab + μa − μb − μ(1)] + 1/2[ab + a − b − (1)] B̂ = 1/2[μab + μb − μa − μ(1)] + (β2 − β1) + 1/2[ab + b − a − (1)] ˆ = 1/2[μab − μb − μa − μ(1)] + 1/2[ab − b − a + (1)] AB In a similar manner as above we can show that