Slide set 12 Stat402 (Spring 2016) Last update: March 28, 2016 Stat 402 (Spring 2016): Slide Set 12 A 23 Factorial in a RCBD • Treatments: A, B, C each at 2 levels; 8 treatment combinations. Thus we need blocks of size: 8. • Consider 2 blocks: Block 1 Block 2 ab c (1) bc c b b (1) bc abc a ab ac a abc ac • The above table shows an example how the treatment combination were randomly applied to experimental units/runs within each block. 1 Stat 402 (Spring 2016): Slide Set 12 A 23 Factorial in a RCBD (Cont’d 1) • Data: Trt (1) a b ab c ac bc abc Block Total Block I 1 2 4 6 4 3 6 10 36 Block II 3 1 5 8 7 3 6 11 44 Trt. Total 4 3 9 14 11 6 12 21 80 2 Stat 402 (Spring 2016): Slide Set 12 A 23 Factorial in a RCBD (ANOVA) • • Computations: 802 16 2 = 400 (SS for the Mean) CF = Total SS = 1 + 22 + · · · + 62 + 112 = 532 Total (Corrected) SS = 532-400=132 Block SS = Trt SS = 362 442 + 8 8 − CF = 404 − 400 = 4 32 212 42 + + · · · + 2 2 2 − CF = 522 − Error SS = 6 (By Subtraction) 400 = 122 ANOVA Table: SV Block Trt Error Total DF 1 7 7 15 SS 4 122 6 132 3 Stat 402 (Spring 2016): Slide Set 12 • Factorial Effects and their Sums of Squares: Now the 7 d.f. for Treatment Sum of Squares is partitioned into 7 single degree of d.f. sums of squares corresponding to the 7 factorial effects: Treatment Combination (1) a b ab c ac bc abc Contrast Divisor for Estimate Estimate of Effect Divisor for SS SS of Effect Observed Total 4 3 9 14 11 6 12 21 I + + + + + + + + 80 16 5.0 16 400 A + + + + 8 8 1.0 16 4 B + + + + 32 8 4.0 16 64 Factorial Effect AB C AC + + + + + + + + + + + + 20 20 0 8 8 8 2.5 2.5 0 16 16 16 25 25 0 BC + + + + 0 8 0 16 0 ABC + + + + 8 8 1 16 4 4 Stat 402 (Spring 2016): Slide Set 12 A 2k Factorial in Incomplete Block Experiments • In factorial experiments the number of treatment combinations increases rapidly as the number of factors and/or the number of levels of each factor increases. • For example, a 27 has 128 different treatment combinations and it is very difficult if not impossible to find blocks of 128 experimental units that are homogenous. (Why?) • Because variation between experimental units within blocks tend to increase as the block size increase (which results in a larger experimental error), it is desirable to keep the block size small. 5 Stat 402 (Spring 2016): Slide Set 12 A 22 Factorial in Blocks of Size 2 • Suppose that we have a 22 factorial experiment but we can obtain blocks of only 2 homogeneous units each. We have 4 treatment combinations (1), a, b and ab, which can be allocated to the 2 blocks in 3 different ways to obtain 3 designs. • We shall consider each of these designs separately: Design 1: Block I (1) ab Block II a b 6 Stat 402 (Spring 2016): Slide Set 12 A 22 Factorial in Blocks of Size 2 (Cont’d design1 ) • We can write the model for the design, assuming one replication of the treatment combinations as follows: y(1) yab ya yb • = = = = µ(1) + β1 + (1) µab + β1 + ab µ a + β 2 + a µ b + β 2 + b Here β1 and β2 represent the respective effects of the two blocks. Using this model, we shall look at the contrasts of the treatment combinations which define the main effects and interactions to see the effect of putting 1/2 of the treatment combinations in one block and the other 1/2 in the other block: 7 Stat 402 (Spring 2016): Slide Set 12 A 22 Factorial in Blocks of Size 2 (Cont’d design 1) A: (a-1)(b+1)  = 1/2[ab + a − b − (1)] = 1/2[µab + β1 + ab + µa + β2 + a − µb − β2 −b − µ(1) − β1 − (1)] = 1/2[µab + µa − µb − µ(1)] + 1/2[ab + a − b − (1)] B: (a+1)(b-1) B̂ = 1/2[ab + b − a − (1)] = 1/2[µab + β1 + ab + µb + β2 + b − µa − β2 −a − µ(1) − β1 − (1)] = 1/2[µab + µb − µa − µ(1)] + 1/2[ab + b − a − (1)] 8 Stat 402 (Spring 2016): Slide Set 12 AB: (a-1)(b-1): d AB = 1/2[ab − a − b + (1)] = 1/2[µab + β1 + ab + µ(1) + β1 + (1) − µa − β2 −a − µb − β2 − b] = 1/2[µab − µa − µb + µ(1)] + (β1 − β2) + 1/2[ab − a − b + (1)] • We can see that if we use this design, then we cannot separate the AB interaction effect from the block effect. The effect of AB interaction is said to be confounded with the block effect. • From above analysis we can see that, if we use Design 1, the effects of A and B are free of the block effect while the effect of AB is not. 9 Stat 402 (Spring 2016): Slide Set 12 A 22 Factorial in Blocks of Size 2 (Cont’d design 2) Design 2: Block I (1) b Block II a ab In this case, the model is given by y(1) yb ya yab = = = = µ(1) + β1 + (1) µ b + β 1 + b µ a + β 2 + a µab + β2 + ab 10 Stat 402 (Spring 2016): Slide Set 12 Hence,  = 1/2[µab + β2 + ab + µa + β2 + a − µb − β1 −b − µ(1) − β1 − (1)] = 1/2[µab + µa − µb − µ(1)] + (β2 − β1) + 1/2[ab + a − b − (1)] B̂ d AB • = 1/2[µab + β2 + ab + µb + β1 + b − µa − β2 −a − µ(1) − β1 − (1)] = 1/2[µab + µb − µa − µ(1)] + 1/2[ab + b − a − (1)] = 1/2[µab + β2 + ab + µ(1) + β1 + (1) − µa − β2 −a − µb − β1 − b] = 1/2[µab − µa − µb + µ(1)] + 1/2[ab − a − b + (1)] If we use this design, then we see that the B effect and the AB interaction effect will be free of block effect and that the main effect A will be confounded with blocks. 11 Stat 402 (Spring 2016): Slide Set 12 A 22 Factorial in Blocks of Size 2 (Cont’d design 3) Design 3: Block I (1) a Block II a ab The model in this case is y(1) ya yb yab = = = = µ(1) + β1 + (1) µ a + β 1 + a µ b + β 2 + b µab + β2 + ab 12 Stat 402 (Spring 2016): Slide Set 12 In a similar manner as above we can show that  = 1/2[µab + µa − µb − µ(1)] + 1/2[ab + a − b − (1)] B̂ = 1/2[µab + µb − µa − µ(1)] + (β2 − β1) + 1/2[ab + b − a − (1)] ˆ = 1/2[µab − µb − µa − µ(1)] + 1/2[ab − b − a + (1)] AB • In this design, A and AB effects are free of block effects and the main effect B is confounded with block effect. Remarks 1. Only one effect is confounded with blocks in each design 2. It is possible to confound a given effect by the choice of the design. 13 Stat 402 (Spring 2016): Slide Set 12 3. We usually try to confound the higher order interaction effects in a given factorial experiment since these are the least important. When an effect is confound it cannot be estimated; hence, no sum of squares exists to test its effect. 4. Each of the above designs is an example of an incomplete block design (as opposed to a complete block design) 5. Confounding can be thought of as a method available for increasing the accuracy of factorial experiments by reducing the block size at the expense of reduced accuracy on estimating some of the effects. Randomization in Incomplete Blocks 1. Given an experimental plan, numbers are assigned to blocks at random. 2. Then treatment combinations are randomly applied to the units within each block. 14