FHTET BIOPESTICIDE PROGRAM ONGOING PROJECTS Gypchek nucleopolyhedrovirus for gypsy moth • Awarded 5-year contract with Sylvar (a Canadian company) to produce 25,000 acre equivalents • Developed a commercially produced carrier – Carrier 038A available through Omnova Solutions (Chester, SC) • Coordinate the use of gypchek as part of the Gypsy Moth Cooperative Suppression, Eradication, and Slowthe-Spread Programs • Maintain state registrations (WI, OH, NC, WV, VA, MD, DE, PA, NJ, NY) TM-Biocontrol-1 nucleopolyhedrovirus for Douglas-fir tussock moth • Developed a commercially produced carrier – Carrier 038A available through Omnova Solutions (Chester, SC) • Maintain state registrations (OR, WA, ID, NM, CA, CO) Bacillus thuringiensis galleriae • Commercially produced boreGONE® (Phyllom BioProducts) • Strain of Bt with high activity against certain species of beetles • Evaluated in lab and small-scale field trials for control of emerald ash borer adults feeding on ash foliage • Limited efficacy due to formulation and application inconsistencies Lecanicillium muscarium • Fungus in commercial product Mycotal® (Koppert Biological Systems, Netherlands) for HWA control • Preliminary trials - aerial application of Mycotal® hemlock forest in TN and ground application in VT • Application of Mycotal® arresteded HWA population growth, control trees HWA continued to expand Verticillium nonalfalfae • V. nonalfalfae first observed causing wilt and mortality to tree-of-heaven in south central Pennsylvania forests • Inoculation studies and host-range susceptibility testing showed that V. nonalfalfae may be a lowrisk agent for controlling tree-of-heaven • Testing different formulations of the fungus to determine which is most effective and usable by land managers Pyricularia grisea phytotoxic metabolites • Developing fungal grass pathogen’s phytotoxic metabolites for inundative control of invasive bufflegrass in environmentally sensitive areas (e.g. Sonoran Desert) • This foliar pathogen already occurs on bufflegrass in North America • Characterize the phytotoxins produced by strains of P. grisea from bufflegrass and examine their ability to cause disease-life damage on bufflegrass host plants