2009 GEM Public Cooperator Report
Mark Campbell, Avinash Karn, Andrew Green, George Shin, Stacy Marshal, Emily Mauch,
Samah Hassan, Suzannes Pitts, Denyse Schrenker (Truman State), Jay-lin Jane, Hongxin Jiang,
(Iowa State University), Don Auger and Yusheng Wu (South Dakota State Unversity)
Chicago ASTA meeting– December 9th 2009.
Introduction
Truman States role as GEM public cooperator has focused on the development of
Amylomaize VII germplasm using GEM breeding material combined with existing mutations
influencing starch properties and carbohydrate metabolism. We feel that our grain quality work
complements other GEM members in the primary directive which includes demonstrating that
the crop diversity maintained by the National Plant Germplasm system, although challenging to
systematically assess, can harbor traits for improving yield, plant health and improving end use
for numerous industrial and nutritional appliations.
Over the past year our work has focused on 1) continuing to better understand starch
properties our GEMS-0067, 2) studying the inheritance of a major modifier gene that appears
(but is not fully confirmed) to be a novel allele of sbe1 (starch branching enzyme 1) which we
shall refer (perhaps prematurely) to as sbe1:gm67 that together with the recessive ae (amyloseextender allele, AKA starch branching enzyme 2b [sbe2b]) allele elevates starch amylose to 70%
(i.e. amylomaize class VII), 3) establishment and training of students of an SSR marker lab at
Truman State University in order to initiate studies on examining the feasibility of conducting
marker assisted selection (MAS) for development of high amylose genotypes in the development
of GEM amylomaize VII germpalsm. In addition, some preliminary studies have begun in order
to 4) evaluate a potentially more rapid means of sample preparation based on DMSO leachate, 5)
more closely evaluate GEM-amylomaize germplasm by Scanning electron microscopy (SEM) of
endoperms starch in-situ. Germplasm development remains top priority with 6) continued
development of novel parent amylomaize VII lines using GEM releases and 7) hybrid evaluation
using these parents. We currently have also 8) developed early generations of materials believed
to be homozygous for ae and waxy (wx) from Truman’s high amylose material and GEM lines
converted by National Starch and Chemical Co (Indianapolis, IN). Much of the material is also
segregating sbe1:gm67. Finally, 9) another paralog of starch branching loci, starch branching 2a,
in which a mutant form (sbe2a:mu) identified by M. Guiltinan (Penn State) is currently being
incorporated into some recent GEM releases in order to observe background effect on expression
of this interesting gene.
Germplasm Development
Amylomaize VII inbreds
For a number of years we have mainly been working toward developing a large set of genetically
diverse amylomaize inbred lines using only GEM materials and usually keeping lines distinctly
of a Stiff Stalk (SS) or Non-stiff Stalk (NS) when possible. When we began this project little
was know of the inheritance of the modifier trait. We now believe that the modifier gene is
likely allelic to sbe1a as mentioned earlier however other factors could very likely be involved.
Our current lines that we have, have been selected from starch analysis and likely possess
sbe1:gm67 allele however future genetic analysis will be necessary to confirm this. A
theoretical scheme describing the genetics involved in the selection process is illustrated in Fig.
1. A list of lines currently believed to possess ae and sbe1:gm67 are shown in Table 1. A
description of the genetations leading up to their development are also shown. Although the
information seems excessively detailed, we need to consider this information expecially when
working with SSR markers for selection in the future. Until now selection has been based
entirely on anlalysis of starch amylose we cannot, at this point, confirm that these lines are fixed
for these two recessive alleles.
Figure 1. Example of the selection
procedure for the development of
amylomaize VII germplasm assuming
genes that are needed for recovery consist
of mainly amylose-extender (ae) and a
putative novel allele of starch branching
enzyme 1 (tentatively described as
sbe1:gm67) originating from Guat209:S13
(A). Location of known starch branching
enzyme paralogs in the maize genome (B)
%
Pedigree
Comprise
d of
GEMS-67
Generation
DKXL370:N11a20-31-1-B-B-SIB/// GUAT209:S13//OH43ae/H99ae
SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae
UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae
UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae
CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae
50
50
50
50
50
08-450-1
08-331 -1
08-364 -1
08-488 -1
08-490 -1
07-348-2
07-243-1
07-267-1
07-370-2
07-380-1
05-318-5 04-498-4
06/07-34 05-93-11
05-95-3
04-132
06-250
05-96-3
06-256
05-81-3
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
DKXL370:N11a20-199-002-B-B-B-Sib////(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae
CH05015:N1502-086-001-B-B-B////(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae
DK212T:N11a12-191-001-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
25
25
25
25
25
25
25
25
25
25
25
25
25
25
25
08-314 -1
08-318 -1
08-320 -1
08-370 -1
08-376 -1
08-388 -1
08-392 -1
08-394 -1
08-410 -1
08-414 -1
08-416 -1
08-422 -1
08-88-9/509
08-93-7/509
08-99-7/473
07-180-1
07-182-2
07-190-1
07-276-1
07-280-1
07-286-1
07-288-2
07-290-2
07-296-1
07-300-2
07-312-2
07-319-1
04GEM00
05GEM06
07GEM02
06/07-11
06/07-12
06/07-26
06-502-4
06-503-1
06-505-1
06-508-3
06-512-1
06-519-1
06-526-4
06-525-2
06-532-2
AR03056:N09-191-001-B-B-B-Sib/////AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43
AR03056:N09-191-001-B-B-B-Sib/////DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT20
BARBGP2:N08a18-332-001-B-B-B/////CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S
CH05015:N1502-086-001-B-B-B/////AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43a
CH05015:N1502-086-001-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
DK212T:N11a12-191-001-B-B-B/////AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43a
DK212T:N11a12-191-001-B-B-B/////AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae
DK212T:N11a12-191-001-B-B-B/////CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13
DKXL370:N11a20-199-002-B-B-B-Sib/////AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT20
DKXL370:N11a20-199-002-B-B-B-Sib/////AR03056:N09-24-1-B-B-B////CH05015:N15-3-1-B-B///GUAT209:S13//OH
DKXL370:N11a20-199-002-B-B-B-Sib/////CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
03-252-2
04-121
03-380-1/484
04-133
04-91
02-4-3
01/02-3 bulk
01-311-5/250 GEM
03-377-7/482
SCR01:N1310-358-1-B-B
UR13085:N0215-14-1-B
03-380-2/486
UR13085:N0215-14-1-B
03-367-5/483
CH05015:N15-3-1-B-B
06-66/255,256 AR03056:N09-24-1-B-B-B
06-67/257
AR03056:N09-182-1-B-B-B
06-78 /255,256 CH05015:N1204-57-1-B-B
06-78 /255,256 05:2/318
02GEM00074
05/06-11
05:3/318
02GEM00076
05/06-13
05:5/318
02GEM00079
05/06-15
05:8/318
02GEM00075
05/06-18
05:12/318
02GEM003g5
05/06-22
05:19/318
03GEM00169
05/06-29
05:26/318
03GEM00182
05/06-36
05:25/318
03GEM00182
05/06-35
05:32/318
04GEM00530
08-85-1/318 04GEM00
08-86-1/409 04GEM00
08-92-2/420 05GEM06
08-96-3/318 05GEM06
08-95-2/518 05GEM06
08-100-1/31807GEM02
08-98-4/317 07GEM02
08-97-2/419 05GEM06
08-89-4/378 04GEM00
08-90-1/314 04GEM00
08-87-2/418 04GEM00
Table 1. Non-stiff stalk (Red) Amylomaize lines and generations leading to their development.
%
Pedigree
Comprise
d of
GEMS-67
Generation
AR16035:S02-615-1-B-B/// GUAT209:S13//OH43ae/H99ae
FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae
2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae
UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae
50
50
50
50
50
08-449-1
08-508 -1
08-516 -1
08-324 -1
08-340 -1
07-343-1
07-387-2
07-391-3
07-236-1
07-248-3
05-315-2
06-258
06-260
06/07-31
06/07-37
04-499-2
05-171-4
05-110-2,1
05-98-1
05-127
03-265-5
04-118
04-96
04-64
04-130
CHRIS775:S1911b-120-1-B-B-B////(CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae
CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
DKB844:S1601-073-001-B-B-B-B-B////UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae
DKB844:S1601-073-001-B-B-B-B-B////UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae
BVIR155:S2012-029-001-B-B////UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-444-001-B-B-Sib////(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H9
GUAT209:S1308a-120-001-B-B////UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-001-B-B-B-B-B-Sib////(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2008c-289-001-B-B/////AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//
25
25
25
25
25
25
25
25
25
25
25
25
25
08-322 -1
08-425 -1
08-430 -1
08-434 -1
08-440 -1
08-534 -1
08-115-1/348
08-116-3/348
08-122-1/347
08-105-1/325
08-118-2/360
08-103-1/322
08-123-1/370
07-211-2
07-320-1
07-324-2
07-326-1
07-334-1
07-330-3
05GEM06
05GEM06
07GEM02
04GEM00
07GEM02
04GEM00
07GEM02
06/07-16
06-533-3
06-536-2
06-539-2
06-548-3
06-543-1
06-71 /253,254
05/06-43
05/06-46
05/06-49
05/06-58
05/06-53
CHRIS775:S1911b-120-1-B
05:33/315
02GEM00442
05:36/315
02GEM04362
05:39/315
03GEM002g1
05:48/315
03GEM00481
05:43/315
03GEM00118
CUBA164:S2012-444-001-B-B-Sib////(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H9
CHIS775:S1911b-120-001-B-B-B-B////(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H
CHIS775:S1911b-120-001-B-B-B-B/////CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//O
CHIS775:S1911b-120-001-B-B-B-B////(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H
CHIS775:S1911b-120-001-B-B-B-B/////CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//O
DKB844:S1601-073-001-B-B-B-B-B/////CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//O
DKB844:S1601-073-001-B-B-B-B-B/////CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//
2088-01_DK212T_S11_F2S4_9157-Blk29/00-sib-B-B-B/////DKB844:S1601-73-1-B-////AR16035:S02-615-1B///GUA
GUAT209:S1308a-047-001-B-B/////CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH4
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
12.5
08-106-1/328
08-109-2/325
08-110-2/425
08-111-2/327
08-112-1/424
08-113-5/425
08-114-5/429
08-117-4/444
08-120-1/425
04GEM00
05GEM06
05GEM06
05GEM06
05GEM06
05GEM06
05GEM06
06GEM01
07GEM02
Table 2. Stiff stalk (Blue) Amylomaize lines and generations leading to their development.
02-33-1
03-375-1/494
03-368-2/490
03-358-2/488
03-379-1/492
01/02-34-BULK
01-327-4/242 G
FS8A(S):S09-362-1-B
CUBA164:S1511b-325-1
2011-01_SE32_S17_F2S4
UR10001:S1813-257-1
Amylose analysis of inbreds
Amylose data was collected from numerous families within lines as is routinely done to confirm through starch
analysis the presence of the modifier. As mentioned in last years report, the summer of 2008 was extremely wet and
combined with our poorly drained Missouri clay loam inbred plants were stunted and suffered although they did
produce grain samples. These conditions also strongly influenced amylase values of starches. In many cases starch
values of lines expected to have amylose at 70 to 75%, instead gave values in the 80’s, 90’s and even over 100%
(relative to commercial standards). Although a considerable amount of analytical data was collected it was decided
that no culling of material was to occur and that all material was planted ear-to-row (2 ears per row in 2008 grown
as 2 rows in 2009) making for a much larger than anticipated nurser (Table 3)
Our nursery again suffered from excessive rain (current rain total this year is 59.5) where average rain for NE
Missouri is 29.5 in. Material will be analyzed and together with marker data perhaps lines with fixed modifier genes
will be identified. As a side comment, the lines surviing the past few years at Truman might possibly have root
systems with unusually good adaptation to poorly drained clay pan soils.
Table 3. 2009 nursery enteries showing lines grown from 2008 all grown again for another
generation of inbreeding without having been discarded because of erounous amylose data
due possible to excessive moisture during the summer of 2008. Material will be subjected
for re-analysis in 2009/2010 although 2009 was also and excessively wet year. The bottom
line is there is a great deal of genetic material that needs to be grown in a good
environment to produce quality ears to make an accurate assessment of amylose data!!
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
5463
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
08- 314
08- 314
08- 315
08- 315
08- 316
08- 316
08- 317
08- 317
08- 318
08- 318
08- 319
08- 319
08- 320
08- 320
08- 321
08- 321
08- 331
08- 331
08- 332
08- 332
08- 333
08- 333
08- 334
08- 334
08- 335
08- 335
08- 336
08- 336
08- 337
08- 337
08- 338
08- 338
08- 339
08- 339
08- 364
08- 364
08- 365
08- 365
08- 366
08- 366
08- 367
08- 367
08- 368
08- 368
08- 369
08- 369
08- 370
08- 370
08- 371
08- 371
08- 372
08- 372
08- 373
08- 373
08- 374
08- 374
08- 375
08- 375
08- 376
08- 376
08- 377
08- 377
08- 378
08- 378
08- 379
08- 379
08- 380
08- 380
08- 381
08- 381
08- 382
08- 382
08- 383
08- 383
08- 384
08- 384
08- 385
08- 385
08- 387
08- 387
08- 388
08- 388
08- 389
08- 389
08- 390
08- 390
08- 391
08- 391
08- 392
08- 392
08- 393
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-24-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
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(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
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(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
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(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
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(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
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AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
08080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808-
394
395
395
396
396
397
397
398
398
399
399
400
400
402
402
403
403
404
404
405
405
406
406
407
407
408
408
409
409
410
411
411
412
412
413
413
414
414
415
415
416
416
417
417
418
418
419
419
420
420
421
421
422
422
423
423
488
488
489
489
490
490
491
491
492
492
493
494
495
495
496
496
497
497
499
499
500
500
501
501
502
502
503
504
504
505
505
506
506
507
507
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
2
1
1
1
2
1
2
1
2
1
2
1
2
1
2
1
2
2
1
2
1
2
1
2
1
2
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 671
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 672
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 673
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 674
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 675
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 676
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 677
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 678
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 679
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 680
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 681
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 682
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 683
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 684
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 685
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 686
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 687
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 688
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 689
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 690
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 691
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 692
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 693
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 694
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 695
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 696
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 697
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 698
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99a 699
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
700
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
701
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
702
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
703
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
704
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
705
FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
706
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
707
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
708
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
709
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
710
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
711
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
712
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
713
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
714
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
715
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
716
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
717
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
718
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
719
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
720
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
721
CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae
722
DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae 723
DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae 724
DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae 725
DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GUAT209:S13//OH43ae/H99ae 726
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727
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728
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729
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730
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731
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732
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733
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734
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735
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736
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737
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739
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740
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741
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742
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743
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744
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761
08- 322
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08- 323
08- 323
08- 324
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08- 327
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CHIS740:S1411a-783-2-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
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CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
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786
787
788
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790
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795
796
797
798
799
800
801
802
803
804
805
806
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808
809
810
811
812
813
814
815
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819
820
821
822
823
824
825
826
827
080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808080808-
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439
439
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440
441
441
442
443
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445
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508
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534
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541
541
542
542
543
543
544
544
545
545
546
546
547
547
548
548
549
549
550
550
2
1
2
1
2
1
2
1
1
2
1
2
1
2
1
2
1
2
1
2
1
2
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1
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CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
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(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
(CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(CUBA164:S2012-444-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
Hybrid Evaluation
Forty experimental entries including a normal check and two commercial amylomaize checks with 37 GEM x GEM
hybrids were evaluated in Iowa and Kirsville, MO (Table 4 and Table 5). Yield data in Iowa again showed that
many GEM x GEM materials out yielded the commercial check varietieis. When compared with the IA yield trial
conducted in 2008, there appears to be three experimental hybrids that performed consistently well these include:
FS8B T:N11a-87-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GEMS-67 X
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GEMS-67
DXKL370:N11a20-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GEMS-67 X
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GEMS-67
DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GEMS-67 X
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GEMS-67
Yield data from Missouri was collected this year but suffered from excessive moisture. The second block in
particular was in a location that was not well drained. The ANOVA did not indicate a significant effect because of
this block. An ANOVA was performed using a one Rep the first block of MO data and as a second rep the mean of
the IA data which revealed a significant effect. Although this might be an unconvential approach it did indicate that
there was some consistency in performance in IA and MO.
Average performance of inbreds are shown in Table 6. It appears that DKXL370:N11a20-199-2-B-BB////DKXL370:N11a20-31-1-B-B-SIB///GEMS-67 and CUBA164:S2012-966-1-B-B////AR16035:S02-6151-B-B///GEMS-67 might serve as good testers for future hybrid evaluations. Special attention should be
given to these lines through starch analysis and SSR marker genotyping to ensure presence of the high
amylose modifier gene in these lines.
Table 4. Hybrid Evaluation:IA
Table 5. Hybrid Evaluation: MO
Table 6. General combining ability of lines from IA yield trial
N
amylomaize VII ss parent line
UR10001:S1813‐257‐1///GUAT209:S13//GEMS‐67 CUBA164:S1511b‐325‐1‐B‐B////AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67
FS8A(S):S09‐362‐1‐B///GUAT209:S13//GEMS‐67 2‐1‐1 2011‐01_SE32_S17_F2S4_9148‐Blk22/00‐sib///GUAT209:S13//GEMS‐67 AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67 UR11003:S0302‐1011‐1‐B‐B////(CUBA164:S2012‐444‐1‐B///GUAT209:S13//GEMS‐67 CHIS775:S1911b‐120‐1‐B‐B‐B////AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67
CHIS740:S1411a‐783‐2‐B‐B////AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67
2011‐01_SE32_S17_F2S4_9148‐Blk22/00‐sib///GUAT209:S13//GEMS‐67 CUBA164:S2012‐966‐1‐B‐B////AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67
CUBA164:S2012‐966‐1‐B///GUAT209:S13//GEMS‐67 CUBA164:S2012‐444‐1‐B‐B‐sib////AR16035:S02‐615‐1‐B‐B///GUAT209:S13//GEMS‐67
CUBA164:S2012‐444‐1‐B///GUAT209:S13//GEMS‐67 2
3
1
8
1
1
6
1
5
8
2
1
1
N
amylomaize VII non‐stiff stalk line
DKXL370:N11a20‐322‐B///GUAT209:S13//GEMS‐67 AR03056:N09‐182‐1‐B‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
DK212T:N11a12‐191‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
FS8B T:N11a‐322‐1‐B‐B /// GUAT209:S13//GEMS‐67 AR03056:N09‐191‐1‐B‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
AR03056:N09‐24‐1‐B‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
SCR01:N1310‐358‐1‐B‐B///GUAT209:S13//GEMS‐67 DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67 CH05015:N1502‐86‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
FS8B(T):N11a‐87‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
SCR01:N1310‐358‐1‐B‐B///GUAT209:S13//GEMS‐67 CH05015:N1204‐57‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
DKXL370:N11a20‐199‐2‐B‐B‐B /// GUAT209:S13//GEMS‐67 UR13085:N0215‐14‐1‐B///GUAT209:S13//GEMS‐67 DREP150:N2011d‐624‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
CH05015:N15‐3‐1‐B‐B///GUAT209:S13//GEMS‐67 DKXL370:N11a20‐36‐2‐B///GUAT209:S13//GEMS‐67 DXKL370:N11a20‐322‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
BARBGP2:N08a18‐332‐1‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
DKXL370:N11a20‐199‐2‐B‐B‐B////DKXL370:N11a20‐31‐1‐B‐B‐SIB///GUAT209:S13//GEMS‐67
2
2
1
2
2
1
6
2
1
2
2
2
1
3
1
1
1
2
1
2
Yield
bu/ac 79.0
102.4
105.0
108.4
111.2
118.3
120.3
122.6
123.1
124.1
135.6
138.2
149.0
MOIST
%
29.1
28.4
27.8
28.6
28.6
23.3
28.9
26.7
29.1
28.1
29.1
26.5
26.7
TWT
Kg
47.3
46.8
47.3
48.2
47.3
47.2
48.0
47.7
48.0
48.1
48.3
49.6
48.4
STAND
SKLDG
RTLDG
50.3
50.3
49.0
49.7
45.5
49.0
47.4
57.5
56.2
53.5
51.5
41.5
55.5
2.0
0.7
2.0
1.4
1.0
0.0
1.5
1.5
1.8
1.8
1.0
2.0
1.0
0.3
0.3
0.0
0.6
0.0
0.0
0.1
13.0
0.8
0.4
0.0
0.0
2.5
Yield
bu/ac 79.0
92.6
94.3
95.9
105.4
108.8
109.8
116.6
120.0
122.4
122.4
123.8
125.3
127.8
128.3
129.0
129.5
131.9
138.2
148.9
MOIST
%
29.1
28.2
30.0
28.1
29.9
30.6
27.1
28.0
29.9
28.7
28.7
28.5
26.1
28.5
29.2
29.2
29.4
28.3
26.5
29.6
TWT
Kg
47.3
47.5
46.7
47.0
46.4
46.1
47.2
49.4
48.9
48.6
48.6
48.0
48.3
49.0
47.9
47.9
48.9
48.1
49.6
49.4
STAND
SKLDG
RTLDG
50.3
51.3
28.0
50.3
52.0
41.5
49.1
56.8
53.5
49.8
49.8
51.5
51.0
51.0
50.5
53.5
60.5
56.3
41.5
56.5
2.0
2.8
2.5
1.3
0.0
1.5
1.8
0.8
2.0
1.3
1.3
2.5
1.5
0.3
1.0
2.0
0.0
2.0
2.0
1.8
0.3
1.0
0.0
0.0
0.0
0.0
2.5
0.5
0.5
0.0
0.0
1.5
1.0
0.3
1.0
0.0
0.0
0.8
0.0
0.0
SSR genotyping of GEM lines used in the developomaize VII lines
This past sping and summer, a great deal
of time and effort, was put toward setting
up a marker lab at Truman for evaluating
the feasibility of using marker assisted
selection from data collected by Yusheng
Wu as part of his theisis dissertations at
SDSU under the guidance of Don Auger.
We have begun by screening
approximately 37 GEM releases used in
our program as recipient lines for
amylomaize VII line development.
Several of these markers were used in the
mapping of the modifier by Yusheng and
are currently being used to genotype
GEM germplasm. Avinash Karn at
Truman identified several other markers
from the Maize Genetic Data Base that
appear to be even more closely linked to
sbe1 that are being used as well. It is our
goal to identify polymorphism among the
markers shown in Figure 2 for these 37
GEM releases. Upon completion a data
base will allow us to examine previoulsly
selected material and determine if cosegregation of these markers with
amylose has been occurring within our
breeding material. If so, marker assisted
selection may very well help to
excellerate the selection process.
Figure 2. SSR markers near sbe1 residing
in 5.03 that is believed to serve as the
major high amylose modifying gene from
GEMS-0067.
0.5 cM
3 cM
GEMN-0117
GEMS-0118
GEMN-0135
GEMN-0137
GEMN-0140
GEMS-0085
GEMS-0086
GEMN-0087
GEMS-0091
GEMN-0097
GEMN-0110
GEMN-0111
GEMN-0117
GEMS-0118
GEMN-0140
GEMN-0140
GEMN-0144
GEMS-0146
GEMS-0149
GEMN-0174
GEMN-0151
GEMN-0154
GEMN-0156
GEMS-0175
GEMS-0180
GEMN-0198
GEMN-0187
GEMN-0191
GEMN-0190
GEMN-0193
GEMN-0197
1
2
1
1
1
1
1
1
1
1
2
3
?
2
1
1
1
1
2
2
2
2
2
2
2
2
3
P2
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
2
0
0
2
2
17
2
-B
-B
B
ib
b
-B
-B
-B
B
B-S
B
Si
-B
-B
-B
-B
BB-B
SI
B-B
-B
B
-B
-B
B1-B
BB-B
-B
B1-B
-B
00
B02
B0
1
1
01
01
B
21-0
1-0
-0
-0
100
00
3
0
3
0
1
9
7
0
3
0
8
8
1
5
-0
-0
02
82
87-7
-0
-5
-2
24
-8
-3
62
a1
08
1a
25
25
13
-0
-3
16
02
a08
41
19
05
18
09
09
03
18
11
:N
:N
S1
:S
:S
:N
:S
:S
:N
0:
P2
):N
09
89
01
56
4
S)
S)
T)
2
T
G
0
0
(
(
(
0
(
7
T
01
10
IS
03
8A
8B
RB
8B
8B
UA
FS
FS
FS
G
UR
UR
AR
CH
FS
BA
2
GEMN-0144
GEMS-0146
GEMS-0149
GEMN-0174
GEMN-0151
GEMN-0154
GEMN-0156
GEMS-0175
GEMS-0180
GEMN-0191
GEMN-0190
P2
21
22
23
24
25
26
27
28
29
2
1
2
1
1
0
0
0
1
2
2
1
2
1
0
0
2
1
2
3
4
3
3
1
1
1
3
1
3
2
1
2
1
18
2
1
19
1
1
20
2
0
2
-B
-B
-B
-7
41
5
B
7
B
B
BB
B
B
B3_
-B
B-B
BB-B
BBB-B
2S
B-B
3B1-B
BB-B
11F
-B
12100
-B
00
01
00
01
N1
300
400
00
01
-0
10
0
0
2
0
7
1
3
8
0
3
6
3
-0
5
0
03
dL3
18
14
84
-0
-1
00
28
050-2
11
8-0
04
KX
02
62
41
21
20
20
81
14
12
03
_D
:N
21
S0
N0
S0
:N
:N
:S
S2
97
7:
5:
0:
:N
50
4:
p3
15
03
2/
1
7
6
2
1
0
0
0
0
0
6
0
G
0
02
05
EP
11
05
R
51
52
I9
31
DI
CH
DR
UR
95
SC
BR
BR
M
NE
UR
2
0
2
2
P2
2
3
4
5
6
7
8
9
10
11
12
13
14
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
1
1
2
1
1
3
3
3
1
1
3
3
3
0
2
2
3
0
2
3
2
2
1
1
2
1
2
1
2
2
1
1
GEMN-0193
GEMN-0197
30
31
32
GEMN-0197
30
GEMN-0193
29
GEMN-0190
26
GEMN-0191
25
GEMN-0187
24
GEMN-0198
23
GEMS-0180
22
GEMS-0175
21
GEMN-0156
20
GEMN-0154
19
GEMN-0151
18
GEMN-0174
17
GEMS-0067
P1
P2
GEMS-0142
20
P1
GEMS-0067
16
H99 ae
19
H99 ae
18
GEMS-0149
15
GEMS-0146
14
GEMN-0140
13
GEMN-0137
GEMN-0135
GEMS-0118
GEMN-0117
12
GEMN-0144
1
P1
GEMN-0111
11
GEMS-0142
GEMN-0097
10
GEMN-0140
GEMS-0091
9
GEMN-0137
GEMN-0087
8
GEMN-0135
GEMS-0086
7
GEMS-0118
GEMS-0085
6
GEMN-0117
GEMN-0083
5
GEMN-0110
GEMN-0081
4
GEMN-0111
GEMS-0079
3
GEMN-0110
GEMN-0097
GEMS-0091
GEMN-0087
GEMS-0086
GEMS-0085
GEMN-0083
GEMN-0081
GEMS-0078
2
GEMS-0149
GEMN-0083
1
GEMS-0079
GEMS-0067
1
GEMS-0146
GEMN-0081
1
GEMS-0078
H99 ae
polymorph
H99 vs
GEMS-67?
P2
GEMN-0144
GEMN-0111
1
GEM 197
17
GEMN-0110
16
GEM 144
15
GEMN-0097
14
GEM 91
13
GEMS-0091
12
GEM 78
11
GEMN-0087
10
GEM 149
9
GEMS-0086
8
GEM 85
7
GEMS-0085
6
GEM 86
5
GEMN-0083
4
GEM 54
score
3
GEMN-0081
Y
2
GEM 87
umc2161
1
GEMS-0079
score
P2
GEMS-0142
umc1870
P1
GEM 137
Y
GEMS-0079
score
GEMS-0078
score
H99
N
GEMS-0078
umc1373
GEMS-0067
score
GEMS-0067
Marker
Y
H99 ae
polymorph
H99 vs
GEMS-67?
Approx.
distance
from sbe1
umc1591
GEMS-0067
polymorph
H99 vs
GEMS-67?
Marker
Approx.
distance
from sbe1
6 cM
GEMS-0067
umc1429
polymorph H99 vs GEMS67?
Marker
Approx.
distance
from sbe1
P1
H99 ae
polymorph
H99 vs
GEMS67?
0.6 cM
Marker
Approx. distance from sbe1
0.6 cM
Marker
Approx.
distance
from sbe1
Table 5. SSR fragments and scoring of markers linked to sbe1 in Bin 5.03
31
1
?
2
32
1
1
?
1
33
33
1
34
35
2
GEMS-0115
GEMS-0092
PI 642763
PI 642763
GEMS-0113
GEMS-0092
score
1
1
1
1
1
1
1
1
1
1
1
1
PI 642763
Y
GEMS-0115
umc1591
1
GEMS-0092
Score
GEMS-0113
N
GEMS-0115
GEMN-0059
umc1371
GEMN-0059
2
GEMS-0113
GEMN-0099
2
GEMN-0099
2
GEMN-0059
GEMS-0093
1
GEMS-0093
2
GEMN-0099
GEMN-0090
1
GEMN-0090
2
GEMS-0093
GEMN-0089
2
GEMN-0089
2
GEMN-0090
GEMN-0088
1
GEMN-0088
1
GEMN-0089
GEMN-0071
2
GEMN-0071
Y
GEMN-0088
GEMS-0063
umc1397
GEMS-0063
Score
GEMN-0071
GEMN-0088
GEMN-0089
GEMN-0090
GEMS-0093
GEMN-0099
GEMN-0059
GEMS-0113
GEMS-0115
GEMS-0092
PI 642763
1
GEMS-0063
2
GEMN-0090
Y
GEMN-0071
GEMN-0090
polymorph
H99 vs
GEMS-67?
umc1429
1
GEMN-0090
Marker
Approx.
distance
from sbe1
score
GEMS-0063
polymorph
H99 vs
GEMS-67?
1
polymorph
H99 vs
GEMS67?
Marker
Approx.
distance
from sbe1
umc1397
GEMN-0090
polymorph
H99 vs
GEMS-67?
6 cM
score
Marker
Approx.
distance
from sbe1
0.6 cM
Marker
Approx.
distance
from sbe1
16
Y
150
bp
100
bp
1
2
2
1
2
2
1
2
2
2
2
2
1
1
2
1
1
1
1
2
2
2
2
2
1
1
1
2
1
1
1
2
1
1
1
3
1
1
3
2
2
2
GEMN-0197
GEMN-0140
15
GEMN-0193
GEMN-0137
14
GEMN-0190
GEMN-0135
13
GEMN-0191
GEMS-0118
12
GEMN-0187
GEMN-0117
11
GEMN-0198
GEMN-0111
10
GEMS-0180
GEMN-0110
9
GEMS-0175
GEMN-0097
8
GEMN-0156
GEMS-0091
7
GEMN-0154
GEMN-0087
6
GEMN-0151
GEMS-0086
5
GEMN-0174
GEMS-0085
4
GEMS-0149
GEMN-0083
3
GEMS-0146
GEMN-0081
2
GEMN-0144
GEMS-0079
1
GEMS-0142
GEMS-0078
P2
GEMS-0067
GEMS-0067
P1
H99 ae
H99 ae
polymorph
H99 vs
GEMS-67?
Marker
Approx.
distance
from sbe1
Table 5. (Cont’d) Fragment and scoring of markers linked to sbe1 in Bin 5.03
1
2
2
2
2
2
2
2
2
2
2
2
1
2
2
2
2
2
Scanning Electron Microscopy (SEM) of amylomaize VII starch granules in
situ
Truman State University has an electron microscope capable of producing detailed images of
endopersm components. There is previous data suggesting that the distribution of granules in the
endosperm can influence properties such as size and amylose content. We therefore, collected
some images of eight amylomaize VII inbreds from 2008 and are listed in Figure 1 along with
whole kernel images of each. Kernels where cut and prepared on stubs for SEM. Certain
locations such as the crown, mid-section and specific distances from the aluerone layer were
used to collect images.
An example of images from one
Figure 3. Amylomaize VII lines selected
of the genoytpes (2011for SEM examination of granual
01_SE32_S17_F2S4_9148shape and size distribution
Blk22/00-sib///GEMS-67).
throughout the endoperm
Similar images were collected
from duplicate kernels to be used
as replications. Our next goal is
to now systematically quantify
characteristics such as granule
density, shape, size, and any other
charactersitcs that might influence
the expression of high amylase
genes. This data will likely
indicate the influence of
endosperm proteins and texture on
the starch. This information will
likely be important to consider
especially regarding 1)starch yield
2) starch charactersitcs and 3)
kernel integrity that could
influence kernel rot and handling
damage.
Figure 4. An example of one genotype (of a total of eight amylomaize
VII GEM lines) used to collect images systematically taken from various
regions of the endosperm.
Development of GEM lines possessing starch-synthesis double-mutants
which are also segregating the high amylose modifier (sbe1:gm67)
There have been a number of studies regarding the interaction of various genes
influencing starch synthesis. Many patents exist on specialty applications of these such
as with the genotype ae wx (waxy). An example includes Patent # 5009911 which
describes the use of ae wx atarch as a thickener in foodstuffs having a pH of 5 or lower
which could be used to replace cross linked modified waxy starches. One of the main
limitations of large scale production of these genotypes especially ae wx is the collapsing
of the kernel resulting in low starch yield and high susceptibility to kernel rots. Our
ojective is to investigate the behavior of these gene in genetic variable GEM material.
Additionally, the influence of sbe1:gm67 will be investigated however genotyping via
SSR markers might be required since it is not yet known what phenotypic effect this
modifier will have. Below is a list of the material we are currently examining (Table 6).
Examples of ears believed to mostly be homozygous for ae and wx are shonw in Fig 5 A
and B. Ears are highly variable in kernel appearance and susceptibily to ear rots. The
material will continue to be inbred and assessed for starch yield and properties.
Table 6. Pedigrees of ae wx germplasm developed from GEM germplasm in an
effort to use the exotic germplasm to improve grain quality traits including starch
yield and kernel fungal pathogen resistance. Waxy GEMS lines converted by
National Starch and Chemical Co. indicated by green font.
08-130-2
08-132-1
08-134-1
08-138-1
08-141-10
08-146-1
08-148-1
08-151-1
08-154-1
DK844:S1601-3-2-B GF0304/GEMS-67
FS8A(S):S09-362-1-B GF0305/////
BARBGP2:NO8a18-332-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR16035:S02-615-1-B GF0301/////
DKB844:S1601-3-2-B-B-B////(FS8A(S):S09-362-1B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
FS8B(T):N1802-525-2-B GM0306/////
UR11003:S0302-1011-1-B-B////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
Cuba164:S2012-966-1-B GF0303/////
BARBGP2:NO8a18-332-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
AR16035:S02-615-1-B GF0301/////
CUBA164:S1511b-325-1-B-B////(FS8A(S):S09-362-1B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
Cuba164:S2012-966-1-B GF0303/////
CHRIS775:S1911b-120-1-B-B-B////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
DK844:S1601-3-2-B GF0304/////
CUBA164:S2012-966-1-B-B////(FS8A(S):S09-362-1B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
FS8A(S):S09-362-1-B GF0305/////
BARBGP2:NO8a18-332-1-B-B////(FS8A(S):S09-362-1B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
08-157-1
08-158-1
08-160-1
08-160-5
08-164-1
08-166-2
08-168-1
08-174-1
08-176-5
08-180-4
08-182-1
08-184-5
08-190-6
08-192-10
08-194-2
08-196-7
08-202-10
08-204-7
08-208-2
SCR01:N1310-509-1-B GM0309/GEMS-67
FS8A(S):S09-362-1-B GF0305/////
CHRIS775:S1911b-120-1-B-B-B////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
FS8A(S):S09-362-1-B GF0305/////
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
FS8A(S):S09-362-1-B GF0305/////
AR03056:N09-182-1-B-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
FS8A(S):S09-362-1-B GF0305/GEMS-67
DK844:S1601-3-2-B GF0304/////
DK212T:N11a12-191-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
Cuba164:S2012-966-1-B GF0303/////
CH05015:N1204-57-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
DKXL370:N11a20-36-2-BGM0308/////
CUBA164:S2012-444-1-B-B-sib////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
Cuba164:S2012-966-1-B GF0303/////
DKXL370:N11a20-199-2-B-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
Cuba164:S2012-966-1-B GF0303/GEMS-67
Cuba164:S2012-966-1-B GF0303/////
AR03056:N09-250-1-B-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
Cuba164:S2012-966-1-B GF0303/////
FS8B(T):N11a-110-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
DK844:S1601-3-2-BGF0304/////
DKB844:S1601-73-1-B-B-B////(FS8A(S):S09-362-1B///GUAT209:S13//OH43ae/H99ae 2-1-1 ) - 1
DKXL370:N11a20-36-2-B GM0308/////
CHIS740:S1411a-783-2-B-B////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
DKXL370:N11a20-36-2-B GM0308/GEMS-67
DKXL370:N11a20-36-2-B GM0308/////
FS8B(T):N11a-87-1-B-B////(CH05015:N15-3-1-BB///GUAT209:S13//OH43ae/H99ae 6-2-2-1) - 1
UR13085:N0215-14-1-B GM0310/////
UR11003:S0302-1011-1-B-B////(CUBA164:S2012-444-1B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
SCR01:N1310-509-1-B GM0309/////
UR11003:S0302-1011-1-B-B////(FS8A(S
UR13085:N0215-14-1-B GM0310/GEMS-67
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
ae / wx
Figure 5. Examples of double mutant (ae wx) ears developed from waxy
GEM line from National Starch crossed with ae lines from Truman that are
likely segregating a high amylose modifier gene (sbe1:gm67). Ears showing
susceptibility rot kernel rot (A) and many showing improved kernel quality
and disease resistance (B).