Bioconversion of Wood Extract
Hydrolyzate: Ethanol, Butanol
and PHA
Shijie Liu , Zhijie Sun, Tingjun Liu, Alan Shupe, Yuanzhen
Wang, Lawrence B. Fitzgerald, and Thomas E. Amidon
Biorefinery Research Institute
Department of Paper and Bioprocess Engineering
SUNY College of Environmental Science and Forestry
1
Imagine a renewable world
2
Outline
Introduction
Ethanol
Butanol
PHA
Conclusion
3
Hemicellulose
„
Common among angiosperm woody biomass:
4
Integrated Biorefinery:
Woody
Biomass
Residual
Woody biomass
Hot-Water
Extraction
Co-Gen
or CHP
Gasification
Feedstock
Extraction
Liquor
Alkaline
Puling
Electricity
and Steam
Reconstituted
Wood Products
Separation
or Co-Gen
Plastics
Adhesives
Solvent
Surface agents
Carbohydrates
Aromatics
…
…
…
Sugars
Food additives:
sugar oligomers
Black
Liquor
Paper products or
Cellulose products
Hydrolysis /
Separation
Acetic
Acid
Fuel
Pellets
Unbleached
Pulp
Bleached Pulp
Methanol
Pulping
Chemicals
Xylitol
Ethanol
Butanol
Acetone
Hydrogen
Lactic Acid
PHA
5
Inhibitors
„
„
Phenolics – orignated from lignin and
extractives of woody biomass
Acetates, formate, furfural, HMF
„
„
„
Detoxification X
More steps in product recovery ?
Microorganism adaptation
f1 (ppm)
Hot-water Wood Extract Hydrolyzate
Fermentation
„
Ethanol
„
„
„
„
E. Coli
Pichia Stipitis
Butanol
„
Clostridium acetobutylicum
„
Clostridium beijerinckii
PHA
„
Burkholderia cepacia
8
Ethanol from Wood Extract Hydrolysates with Pichia Stipitis
20
Ethanol Concentration, g/L
NRRL Y-11543
25% Hydrolysate
50% Hydrolysate
75% Hydrolysate
100% Hydrolysate
15
10
5
0
0
1
2
3
4
5
6
7
8
Time, days
9
Adaptation of fermenting microorganism
hydrolysate
challenge
overnight culture
FBR5
overnight culture
fermentation
medium
liquid medium
compare
with parent
dilutions
plating
selective medium
10
Two new fermenting strains
„
„
E. Coli fbhw
P. Stipitis hw
Ethanol by E. Coli FBHW
40
120
30
100
20
80
60
Reducing sugar
Ethanol
Cell
40
10
0
20
0
1
2
Time, day
3
4
Ethanol, g/L or cell, 0.1g/L
Reducing sugar concentration, g/L
140
Solvent concentration(g/L)
Butanol Production ATCC 824
12
10
Total solvent
8
butanol
6
4
2
0
0
40
80
120
Time (hour)
acetone
ethanol
160
13
Conclusions
Wood extract hydrolyzates are reactive
Ethanol production reached 35 g/L for both E.
Coli and P. Stipitis
Butanol production reached close to 10 g/L
PHA production started.
Challenges
Feedstock variation
Butanol concentration still low
PHA – not extracelluar product
14
Acknowledgements
￭ Christopher D. Wood
„
Dave Kiemle
„
Raymond Appleby ￭ Ruofei Hu
„
Jennifer Putnam ￭ Kathryn Gratien
„
Others
15
Acknowledgements
„
„
NYSERDA
US DOE
16
Thanks!
17