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AcroPrep™ Advance 96
Multi-Well Filter Plates
• Provide improved consistency and minimal sample loss in a wide variety of sample preparation
and detection applications.
• Meet specifications of the Society for Biomolecular Screening (ANSI/SBS x-2004) for
multi-well plates.
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Ordering Information
96-Well, 350 µL
Part Number
8019
8029
8039
8027
8031
8033
8034
8035
8036
8040
8075
Description
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
AcroPrep Advance
Filter
Filter
Filter
Filter
Filter
Filter
Filter
Filter
Filter
Filter
Filter
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
Plate
with
with
with
with
with
with
with
with
with
with
with
0.2 µm Supor®
0.45 µm Supor
1.2 µm Supor
PP/PE, 30 – 40 µm
1 µm Glass Fiber
Omega™ 3K MWCO
Omega 10K MWCO
Omega 30K MWCO
Omega 100K MWCO
3 µm GF/1.2 µm Supor
3 µm GF/ 0.2 µm Supor
Packaging
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
10/pkg
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Accessories
Part Number
5017
5016
5015
5014
5225
5226
8001
Description
Multi-well Plate Vacuum Manifold
Replacement Accessory Kit
(includes O-ring, gasket, and Allen wrench)
350 µL Receiver Plate Spacer Block
1 mL Receiver Plate Spacer Block
Adapter Collar for Centrifugation
Adapter Collar for PCR Receiver Plate
Multi-well Plate Lids
1
Packaging
1/pkg
1/pkg
1/pkg
1/pkg
2/pkg
2/pkg
10/pkg
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Table of Contents
Section
Introduction
Page
12
Specifications
13
Instructions for Use
14
Chemical Compatibility
15
Microporous Media
16
Ultrafiltration Media
18
Complementary Products
10
Warning
10
Note: The procedures herein are intended only as a guide. Users should always verify product
performance with their specific applications under actual use conditions. If you have questions
about the information presented in this guide, please contact our Technical Service Department
at LabCustomerSupport@Pall.com.
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Introduction
The AcroPrep Advance line of multi-well filter plates is the next generation line of filter plates from
Pall Life Sciences. AcroPrep Advance filter plates are designed to provide superior performance in
a multitude of sample prep and screening applications. These multi-well filter plates have been
optimized to support the stringent requirements of high throughput applications. AcroPrep Advance
filter plates address specific application challenges, providing performance advantages in your
most critical applications.
AcroPrep Advance filter plates are designed in accordance with the standards set forth by the
Society of Biomolecular Screening (ANSI/SBS x-2004) for multi-well plates. The plate has a rigid
construction, which facilitates ease of use in automated systems. The rigidity of the plate prevents
it from flexing and jamming in robotic processing units.
The sealing mechanism used in the AcroPrep Advance filter plate individually seals each well to
help prevent cross-talk or cross contamination of samples. The advanced fluid director on the
bottom of the plate also minimizes cross contamination of the filtrate during processing.
The plate is constructed from chemically resistant, biologically inert polypropylene which means
that it is low binding to nucleic acids and proteins. The nature of the polypropylene housing also
provides durability when using harsh organic solvents and will not contribute to unwanted
extractables. Additionally, the materials of construction have been tested to ensure that they do
not contribute to ion suppression or enhancement.
Note: For filtrate applications that include volatile organic solvents in the plate, a pre-test to
confirm chemical compatibility with downstream application is recommended.
2
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Specifications
Materials of Construction
Media Type
Media Description
Glass Fiber†, ††
Borosilicate Glass without Binder
Non-woven Media Polypropylene/Polyethylene (PP/PE) Blend
Omega
Modified Polyethersulfone
Supor
Hydrophilic Polyethersulfone
†
Pore Size/MWCO
1.0 µm, 3.0 µm
30-40 µm
3K,10K,30K,100K
0.2 µm, 0.45 µm, 1.2 µm
When using glass fiber with scintillant, we recommend a pre-test before use.
Glass fiber media is supported with a polypropylene support layer.
††
Plate Housing
Polypropylene
Plate Lid
Polystyrene
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Dimensions
Plate design and dimensions meet the recommended specifications of the Society for
Biomolecular Screening (ANSI/SBS x-2004) for multi-well plates used in robotic systems.
96-Well, 350 µL
Length: 12.78 cm (5.03 in.)
Width: 8.55 cm (3.36 in.)
Height (with lid): 1.75 cm (0.69 in.)
Height (without lid): 1.44 cm (0.56 in.)
Specification
Well Bottom Area
Maximum Well Volume
Recommended Working Volume
Recommended Operating Vacuum
Recommended Centrifugal Force
96-Well, 350 µL
0.25 cm2
350 µL
300 µL
≥ 25.4 cm Hg (10 in. Hg)
1,500 x g
3
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Specifications (continued)
Typical Membrane Performance
Vacuum Filtration
Membrane
0.2 µm Supor
0.45 µm Supor
1.2 µm Supor
1.0 µm Glass Fiber
3.0 µm Glass Fiber / 0.2 µm Supor
3.0 µm Glass Fiber / 1.2 µm Supor
PP/PE
10K Omega
30K Omega
100K Omega
Processing Time (sec)
9
5
2
2
9
2
1
20 min
8 min
4 min
Hold-up Volume (µL)
8
6
5
19
13
17
4
5
6
7
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Centrifugal Filtration
Membrane
1.2 µm Supor
3K Omega
10K Omega
30K Omega
100K Omega
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Processing Time (min)
<2
45
8
8
5
Hold-up Volume (µL)
3
2
2
2
2
Note: Processing time and hold-up volume analysis was performed with 300 µL of water loaded
into each well. Note that centrifugation consistently results in lower hold-up volume. For applications
where sample dilution and/or recovery are critical, centrifugation is recommended.
Instructions for Use
The AcroPrep Advance line of filter plates are designed for use in both manual and automated
applications. The plate dimensions allow them to be processed in a centrifuge, on most standard
vacuum manifolds, and on robotic equipment from the following manufacturers: Tecan U.S.,
Qiagen Inc., Tomtec, PerkinElmer, Beckman Coulter Inc, Titertek, Agilent Technologies, Waters,
Hamilton Company, Protedyne, and Caliper Life Sciences.
Vacuum Filtration Procedure
1. Place a receiver plate in the collection area of the vacuum manifold.
2. Place the plate on top of the vacuum manifold.
3. Add the sample/solution to the filter plate, and incubate if required.
4. Apply vacuum. Recommended vacuum is 25.4 cm Hg (10 in. Hg) or greater.
4
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Instructions for Use (continued)
5. Release vacuum from the manifold with pressure release valve. DO NOT RELEASE VACUUM
BY PULLING THE CORNER OF THE PLATE AS IT WILL DEGRADE THE MANIFOLD GASKET.
6. If required add wash solutions and filter as needed.
Centrifugation Filtration Procedure
1. Add the solution to the filter plate and incubate, if required.†
2. For optimal alignment, place an adaptor collar (PN 5225) on top of a receiver plate.
3. Place the filter plate on top of the receiver plate with collar.
4. Place entire assembly into rotor designed for microplates. Make sure to balance plates in the rotor.
5. Centrifuge at 1,500 x g for 1 minute. (Longer times are required for viscous solutions and
ultrafiltration applications.)
6. Remove plates from rotor and proceed as required.
†
Refrain from allowing the outlet tips of the plate to be in contact with any surface while liquid is in
the wells to prevent liquid flow due to wicking.
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Chemical Compatibility
R = Resistance
No significant change was observed in flow rate or integrity of the membrane, nor visible
indication of chemical attack.
L = Limited Resistance
Moderate changes in physical properties or dimensions of the membrane were observed. The
filter may be suitable for short term, non-critical use. Filter plate may be suitable for short term
exposure at low pressures and ambient temperatures.
N = Not Resistant
The membrane in the AcroPrep Advance filter plate is unstable and is not recommended for use.
I = Insufficient Data
Information is not available. Trial testing is recommended.
Caution
Alcohol residues that are allowed to dry on a filter may cause stress cracks. Therefore, Pall Life
Sciences recommends that filters used in alcohol processing should remain alcohol wet, or should be
flushed with copious quantities of water to remove residuals prior to drying and subsequent reuse.
5
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Chemical Compatibility – Microporous Media
Test Methods
The data presented in this chart is a compilation of testing by Pall Life Sciences with certain
chemicals, manufacturer’s data, or compatibility recommendations from the Compass Corrosion
Guide by Kenneth M Pruett. This data is intended to provide expected results when filtration
devices are exposed to chemicals under static conditions for 48 hours at 25 ºC (77 ºF), unless
otherwise noted.
This chart is intended only as a guide. Accuracy cannot be guaranteed. Users should verify
chemical compatibility with specific filter under actual use conditions. Chemical compatibility with
a specific filter under actual use conditions is affected by many variables including temperature,
pressure, concentration, and purity. Various chemical conditions prevent complete accuracy.
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Glass Fiber Media
Supor (PES) Membrane
PP/PE Blend
Acids
N
N
R
R
R
R
L
L
R
R
R
R
R
R
R
R
R
N
N
-
R
R
R
R
R
R
R
L
I
R
R
6
Acetic acid, glacial
Acetic acid, (90%)
Acetic acid, (30%)
Acetic acid, (10%)
Hydrochloric acid, conc. (35%)
Hydrochloric acid, 6N (20%)
Hydrochloric acid, 1N (3.3%)
Nitric acid, conc. (67%)
Nitric acid, 6N (27%)
Sulfuric acid, conc. (96%)
Sulfuric acid, 6N (16%)
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Chemical Compatibility – Microporous Media (continued)
Glass Fiber Media
Supor (PES) Membrane
PP/PE Blend
Alcohols
Bases
R
L
R
R
R
R
R
R
R
N
N
N
N
R
R
R
R
R
R
R
R
R
R
N
R
R
R
R
R
R
N
I
I
Amyl alcohol
Benzyl alcohol
Butanol
Ethanol
Isopropanol
Methanol
Ammonium hydroxide, 3N (5.7%)
Ammonium hydroxide, 6N (11.4%)
Potassium hydroxide, 3N (15%)
Sodium hydroxide, 3N (11%)
Sodium hydroxide, 6N (22%)
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Esters
Ethers
Glycols
Aromatics
Halogenated Hydrocarbons
L
L
R
R
R
N
L
R
R
R
L
L
L
N
L
N
N
R
10:40 PM
R
R
R
N
R
N
R
N
R
R
R
R
R
R
R
N
N
N
R
R
R
R
R
R
R
R
L
I
R
R
R
R
R
R
R
R
R
R
R
Page 15
Amyl acetate
Butyl acetate
Cellosolve acetate
Ethyl acetate
Isopropyl acetate
Methyl acetate
Ethyl ether
Tetrahydrofuran
Tetrahydrofuran/water (50/50,v/v)
Ethyl glycol
Glycerol
Propylene glycol
Benzene
Toluene
Xylene
Carbon tetrachloride
Chloroform
Ethylene dichloride
Methylene chloride
Tetrachloroethylene
7
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Chemical Compatibility – Microporous Media (continued)
Glass Fiber Media
Supor (PES) Membrane
PP/PE Blend
Ketones
Oils
R
N
R
L
R
R
N
N
N
R
R
R
R
R
R
R
R
R
Acetone
Cyclohexanone
Methyl ethyl ketone (MEK)
Methyl isobutyl ketone
Cottonseed
Peanut
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Miscellaneous
R
R
R
R
N
L
L
-
10:40 PM
N
N
N
R
R
L
R
N
R
R
R
R
R
R
R
R
R
R
Page 17
Acetonitrile
Dimethyl formamide (DMF)
Dimethyl sulfoxide (DMSO)
Formaldehyde, 37%
Formaldehyde, 4%
Hexane, dry
Kerosene
Pyridine
18 Megohm water
Chemical Compatibility – Ultrafiltration Media
Test Methods
The data is a compilation by Pall Life Sciences with the listed chemicals. Chemical compatibility
was determined with discs. Flux rates and solute retention was determined before and after a
two-hour exposure to the indicated chemical. If membrane integrity was unchanged, the chemical
was deemed compatible.
This chart is intended only as a guide. Accuracy cannot be guaranteed. Users should verify
chemical compatibility with specific filter under actual use conditions. Chemical compatibility with
a specific filter under actual use conditions is affected by many variables including temperature,
pressure, concentration, and purity. Various chemical conditions prevent complete accuracy.
Pall Omega ultrafiltration membranes have been evaluated for compatibility with many common
laboratory buffers and solvents over a wide pH range.
8
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Chemical Compatibility – Ultrafiltration Media (continued)
Omega Membrane
Acids R
R
R
R
R
R
R
R
N
R
Alcohols R
R
R
R
R
R
Acetic acid, (25.5%)
Citric acid (1%)
Formic acid (5%)
Hydrochloric acid (0.1N at 50 °C)
Hydrochloric acid (1N at 25 °C)
Lactic acid (5%)
Nitric acid (≤1%)
Oxalic acid (1%)
Phosphoric acid (1N)
Sulfuric acid (1%)
Butanol (70%)
Butyl cellosolve (10%)
Ethanol (70%)
Isopropyl alcohol (25%)
Mercaptoethanol (0.1%)
Methyl alcohol (25%)
Omega Membrane
Bases N
N
R
R
Esters N
R
R
R
Ethers N
R
Hydrocarbons R
N
R
R
Amines
Ammonium hydroxide (5%)
Sodium hydroxide (1N at 25 °C)
Sodium hydroxide (1N at 50 °C)
Aliphatic & aromatic esters
Butyl acetate (40%)
Ethyl acetate (≤ 30%)
Isopropyl acetate (1%)
Ethers
Tetrahydrofuran (5%)
Toluene (1%)
Aromatic & chlorinated hydrocarbons
Chloroform (0.8%)
Methylene chloride (1%)
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Ketones
Miscellaneous
R
N
R
R
R
R
R
N
N
R
R
R
R
R
R
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Acetone (≤ 30%)
Ketone
Methyl ethyl ketone (MEK) (1%)
Phenol (0.5%)
Acetonitrile (≤ 40%)
Ammonium chloride (1%)
Alconox (1%)
Calcium chloride (5%)
Dimethyl acetamide (DMAC) (≤ 40%)
Dimethyl formamide (≤ 40%)
Dimethyl sulfoxide (≤ 40%)
Disodium salt of EDTA (10%)
Formaldehyde (1%)
Glutaraldehyde (0.5%)
Glycerine (50%)
Page 19
Miscellaneous
9
R
R
R
R
R
R
N
R
R
R
R
R
R
R
R
Guanidine HCl (6M)
Hydrogen peroxide (1%)
N-Methylpyrrolidone (1%)
Phosphate buffer (1M, pH 8.2)
Sodium azide (1%)
Sodium chloride (5% at 50 °C)
Sodium deoxycholate (5%)
Sodium dodecyl sulfate (0.1M)
Sodium hypochlorite (0.1%)
Sodium nitrate
Terg-a-zyme (1%)
Tris buffer (1M, pH 8.2)
Triton X-100 (0.002M)
Urea (25%)
Ultrasil 11 (1%)
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Complimentary Products
• AcroWell™ 96-well Filter Plates with BioTrace™ NT and BioTrace PVDF membranes exhibit
high binding capacities for proteins and nucleic acids.
• Centrifugal Devices – Pall Life Sciences offers centrifugal devices for processing the following
sample volumes:
Device
Nanosep® Device
Microsep™ Device
Macrosep® Device
Jumbosep™ Device
Sample Volume
up to 0.5 mL
0.5 mL to 3.5 mL
3.5 mL to 15 mL
15 mL to 60 mL
• BioTrace, Biodyne®, and FluoroTrans® Transfer Membranes offer precise performance and
compatibility with nearly every detection system available.
• Enchant™ Abundant Protein Removal Kits offer simple, easy to use affinity resin devices for
the removal of albumin and IgG.
• Chromatography Resin for the purification of biomolecules and compounds. Available
Chemistries include Affinity, Ion Exchange, Size Exclusion, Mixed-mode, and Hydroxyapatite.
• AcroSep™ Chromatography Columns are pre-packed with Pall Life Sciences chromatography
resin for the purification of proteins using syringe, pump, or automated chromatography systems.
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WARNING
Employment of the products in applications not specified, or failure to follow all
instructions contained in this product information insert, may result in improper functioning
of the product, personal injury, or damage to property or the product. See Statement of
Warranty in our most recent catalog.
ATTENTION
L’utilisation de nos produits dans des applications pour lesquelles ils ne sont pas spécifiés
ou le non-respect du mode d’emploi qui figure sur ce document, peut entrainer un
disfonctionnement du produit, endommager le produit ou d’autres biens matériels ou
représenter un risque pour l’utilisateur. Se référer à la clause de garantie de notre
catalogue le plus récent.
ACHTUNG
Der Einsatz dieses Produktes in Anwendungen für die es nicht spezifiziert ist, oder das
Nichtbeachten einiger, in dieser Bedienungsanleitung gegebenen Hinweise kann zu einem
schlechteren Ergebnis, oder Zerstörung des Produktes oder anderer Dinge oder gar zu
Verletzungen führen. Beachten Sie auch unsere Garantiebedingungen im aktuellen Katalog.
10
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ADVERTENCIA
El uso de este producto en aplicaciones no especificadas o el no considerar las
instrucciones indicadas en la hoja de información del producto puede ocasionar un mal
funcionamiento del producto, daños en las instalaciones o en el producto y riesgo para el
personal del laboratorio. Consulte el apartado de Garantía en nuestro último catálogo.
ATTENZIONE
L’impiego dei prodotti in applicazioni non specificate, o il mancato rispetto di tutte le
istruzioni contenute nel presente bollettino tecnico, potrebbero portare ad un utilizzo
improprio del prodotto, ferire gli operatori, o danneggiare le caratteristiche del prodotto
stesso. Consultare la dichiarazione di garanzia pubblicata nel nostro più recente catalogo.
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11
Page 23
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Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520
(+)800.PALL.LIFE
734.665.0651
734.913.6114
USA and Canada
Outside USA and Canada
phone
fax
Visit us on the Web at www.pall.com/lab
E-mail us at LabCustomerSupport@pall.com
Pall,
, AcroPrep, AcroSep, AcroWell, Biodyne,
BioTrace, Enchant, FluoroTrans, Jumbosep, Macrosep,
Microsep, Nanosep, Omega, and Supor are trademarks
of Pall Corporation. ® indicates a trademark registered
in the USA.
© 2009, Pall Corporation, 10/09
Page 24
Australia, Cheltenham, VIC, 03 9584 8100
Austria, Wien, 00 1 49 192 0
Canada, Ontario, 905-542-0330
Canada, Québec, 514-332-7255
China, P.R., Beijing, 86-10-6780 2288
France, St. Germain-en-Laye, 01 30 61 32 32
Germany, Dreieich, 06103-307 333
India, Mumbai, 91 (0) 22 67995555
Italy, Buccinasco, +3902488870.1
Japan, Tokyo, 03-6901-5800
Korea, Seoul, 82-2-560-8711
Malaysia, Selangor, +60 3 5569 4892
Poland, Warszawa, 22 510 2100
Russia, Moscow, 5 01 787 76 14
Singapore, 65 6 389-6500
South Africa, Johannesburg, +27-11-2662300
Spain, Madrid, 91-657-9876
Sweden, Lund, (0)46 158400
Switzerland, Basel, 061-638 39 00
Taiwan, Taipei, 886 2 2545 5991
Thailand, Bangkok, 66 2937 1055
United Kingdom, Farlington, 02392 302600
PN 89460A