11-7022_ChromPoster_SS_PALL_Layout 1 7/25/11 4:37 PM Page 1
Pall Chromatography Media
Versatile offering
for purification
of biomolecules
• Size Exclusion
(Gel Filtration)
• Ion Exchange
• Affinity Purification
• Mixed-Mode
• Solvent Detergent
Removal
• Hydroxyapatite
Chromatography continues to be an essential technology for the purification
of biomolecules. Pall offers an extensive portfolio of media for affinity, ion exchange,
mixed-mode, size exclusion, solvent detergent removal, and hydroxyaptite
chromatography. Our products cover research needs, scale-up, and polishing.
Depending on your specific application needs, Pall has chromatography
solutions in sorbent and/or membrane form. We offer flat sheet membranes,
bulk sorbents, pre-packed columns, and media incorporated into specific
product housings.
Our chromatography media can be used for purification of biomolecules
(e.g., nucleic acids, proteins) and compounds on the research and process
scale. Our sorbents are useful in proteomic sample preparation applications,
as well as in laboratory bioprocessing development and scale-up work.
For the full listing of chromatography technologies offered by Pall, please
refer to the Pall Laboratory Filtration, Separation and Detection Products
Catalog or visit www.pall.com/lab.
Chromatography Product Chart
Chromatography
Type
Size Exclusion
(Gel Filtration)
Separation by
Molecule Size
Bulk Sorbent
Average
Particle Size
Capacity
Working pH
100 µm
NA
3-10
Product
Part Number
Size
Description
Applications
Exclusion Limit
750 KD
Ultrogel® AcA 34
23015-025
23015-019
23022-024
23022-015
23019-023
23019-011
24892-022
24892-010
100 mL Bottle
1000 mL Bottle
100 mL Bottle
1000 mL Bottle
100 mL Bottle
1000 mL Bottle
100 mL Bottle
1000 mL Bottle
Polymeric sorbents for size exclusion composed
of polyacrylamide and agarose, characterized by
narrow particle size and pore size distribution.
AcA 202 can be used for desalting.
Fractionation Range
20-350 KD
200 KD
10-130 KD
90 KD
5-70 KD
22 KD
1-15 KD
Trisacryl® GF05 M
25914-060
25914-037
100 mL Bottle
1000 mL Bottle
Highly hydrophilic copolymers designed
for medium pressure gel filtration.
Can be used for desalting.
60 µm
Desalting capacity
33% gel volume
1-11
Exclusion Limit
3 KD
Fractionation Range
200-2500 Da
Q HyperCel™
20196-012
20196-024
20196-036
20196-048
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Strong anion exchanger with different salt
sensitivity and selectivity for proteins than
other conventional and new generation ion
exchangers. Industry-scalable sorbent
designed for high productivity protein
capture and intermediate separation steps.
75 µm
160-198 mg/mL1
1-13
Purification of recombinant proteins,
monoclonal and polyclonal antibodies,
plasma derivatives, vaccines or other
biopharmaceuticals
S HyperCel
20195-013
20195-025
20195-037
20195-049
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Strong cation exchanger with different salt
sensitivity and selectivity for proteins than
other conventional and new generation ion
exchangers. Industry-scalable sorbent
designed for high productivity protein
capture and intermediate separation steps.
75 µm
134-190 mg/mL2
1-13
Purification of recombinant proteins,
monoclonal and polyclonal antibodies,
plasma derivatives, vaccines or other
biopharmaceuticals
Q Ceramic HyperD® F
20066-098
20066-031
20066-023
20066-015
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Strong anion exchanger that employs a high
capacity hydrogel polymerized within the
large pores of a rigid ceramic bead.
50 µm
> 85 mg/mL3
2-12
Protein concentration, protein separation,
contaminant removal
S Ceramic HyperD F
20062-089
20062-030
20062-022
20062-014
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Strong cation exchanger that employs a high
capacity hydrogel polymerized within the
large pores of a rigid ceramic bead.
50 µm
> 75 mg/mL4
2-12
Protein concentration, protein separation,
contaminant removal
DEAE Ceramic HyperD F
20067-070
20067-039
20067-021
20067-013
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Weak anion exchanger that employs a high
capacity hydrogel polymerized within the
large pores of a rigid ceramic bead.
50 µm
> 85 mg/mL3
2-12
Protein concentration, protein separation,
contaminant removal
CM Ceramic HyperD F
20050-084
20050-035
20050-027
20050-019
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Weak cation exchanger that employs a high
capacity hydrogel polymerized within the
large pores of a rigid ceramic bead.
50 µm
> 60 mg/mL5
2-12
Protein concentration, protein separation,
contaminant removal
AcroSep™
Columns
CM Ceramic HyperD F
S Ceramic HyperD F
Q Ceramic HyperD F
DEAE Ceramic HyperD F
IEX Variety Pack
20050-C001
20062-C001
20066-C001
20067-C001
IEXVP-C001
1 mL Column, 5/pkg
Pre-packed with patented ceramic HyperD
"gel-in-a-shell" sorbents for chromatographic
separations. Offers extremely high dynamic
binding capacities at fast flow rates.
50 µm
> 60 mg/mL5
> 75 mg/mL4
> 85 mg/mL3
> 85 mg/mL3
See above
2-12
Protein concentration, protein separation,
contaminant removal
Devices
AcroPrep™ Advance Filter
Plate with Mustang® Q,
350 µL
AcroPrep Advance Filter
Plate with Mustang Q,
1 mL
AcroPrep Advance Filter
Plate with Mustang S,
350 µL
AcroPrep Advance Filter
Plate with Mustang S,
1 mL
8071
10/pkg
Strong anion exchange membrane in a 96-well
filter plate configuration.
N/A
N/A
N/A
Protein concentration, protein separation,
contaminant removal
8171
5/pkg
8072
10/pkg
8172
5/pkg
MSTG25Q6
10/pkg
N/A
N/A
Protein concentration, protein separation,
contaminant removal
MSTG25S6
10/pkg
Acrodisc Mustang E
Chromatography Unit
MSTG25E3
10/pkg
Highly cross-linked quaternized amine membrane
in a syringe filter configuration.
Constructed to be chemically stable over a broad
range of conditions.
DNA: 3.6 mg/Acrodisc unit
BSA: 10 mg/Acrodisc unit
Lysozyme: 8 mg/
Acrodisc unit
Human lgG: 11 mg/
Acrodisc unit
500,000 EU/Acrodisc unit
Affinity
Purification
Separation Using
Specific Ligands
Blue Trisacryl M
25896-051
25896-045
25896-010
25896-028
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
An affinity sorbent with the basic matrix
of Trisacryl GF2000, a macroporous
non-ionic sorbent on which Cibacronu
blue is covalently immobilized.
60 µm
HSA: 10-15 mg/mL6;
BSA: 5-7 mg/mL6
1-10
Albumin depletion, purification of enzymes
and proteins, such as kinases, interferons,
and some coagulation factors
Bulk Sorbent
Protein A Ceramic
HyperD F
20078-036
20078-028
20078-010
20078-044
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Recombinant Protein A is immobilized to a
hydrogel within a porous ceramic bead.
50 µm
> 30 mg/mL7
2-11
IgG purification/depletion
Heparin HyperD M
20029-062
20029-039
20029-021
20029-013
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Composed of a porous rigid mineral
bead containing heparin (porcine)
bound hydrogel filled pores.
80 µm
> 25 mg/mL8
3-13
Purification of coagulation factors,
lipoproteins, growth hormones, growth
factors, nucleic acid binding enzymes
IMAC HyperCel
20093-069
20093-010
20093-028
20093-036
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Uses tridentate IDA as a chelating agent.
This ligand is immobilized on the HyperCel
base sorbent.
90 µm
Metal ion capacity:
40-70 µmol Cu2+/mL
Ionic capacity:
90-140 µeq/mL
3-12
Purification of HIS-tagged proteins,
antibodies, and prefractionation of
complex protein mixtures
Lysine HyperD
20059-058
20059-036
20059-028
20059-010
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Comprised of a porous rigid mineral
bead containing lysine (L-lysine)
bound hydrogel filled pores.
70 µm
N/A
3-13
Purification of glycoproteins and other
biological molecules that bind to Lysine
Protein A Ceramic
HyperD F
20078-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
50 µm
> 30 mg/mL7
2-11
IgG purification/depletion
IMAC HyperCel
20093-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
90 µm
40-70 µmol Cu2+/mL
3-12
Purification of His-tagged proteins; isolation
of phospho-proteins and peptides.
Blue Trisacryl M
25896-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
60 µm
10-15 mg/mL6
1-10
Albumin depletion; purifcation of coagulation
factors and lipoproteins
MEP HyperCel
12035-069
12035-010
12035-028
12035-036
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
High capacity, high selectivity sorbent.
In contrast to Protein A sorbents, IgG
binding on MEP HyperCel is essentially
independent of subclass or species.
"Weakly binding" variants (e.g.,
murine IgG, rat IgG) are well retained.
90 µm
≥ 20 mg/mL9
2-12
Charge
Purification/depletion of polyclonal and
monoclonal antibodies from most species
HEA HyperCel
20250-012
20250-026
20250-033
20250-041
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Based on a combination of electrostatic
and hydrophobic properties of the
protein and ligands. Has an aliphatic
(n-hexylamine) ligand which has lower
hydrophobicity than PPA.
90 µm
40-60 mg/mL11
2-12
Unique industry-scalable sorbents designed
for protein capture and impurity removal in
a biopharmaceutical environment. Provides
different selectivites not accessible with
traditional ion exchange or HIC.
PPA HyperCel
20260-015
20260-025
20260-030
20260-040
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
Based on a combination of electrostatic
and hydrophobic properties of the
protein and ligands. Has an aromatic
(phenylpropylamine) ligand which is
highly hydrophobic.
90 µm
40-60 mg/mL11
2-12
Unique industry-scalable sorbents designed
for protein capture and impurity removal in
a biopharmaceutical environment. Provides
different selectivites not accessible with
traditional ion exchange or HIC.
MEP HyperCel
12035-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
90 µm
> 20 mg/mL9
2-12
Purification of polyclonal and monoclonal
antibodies from most species
HEA HyperCel
20250-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
90 µm
40-60 mg/mL11
2-12
Protein capture and impurity removal;
alternative to traditional ion exchange and
HIC sorbents
PPA HyperCel
20260-C001
1 mL Column, 5/pkg
Convenient pre-packed column for discovery
phase and small scale purifications.
90 µm
40-60 mg/mL11
2-12
Protein capture and impurity removal;
alternative to traditional ion exchange and
HIC sorbents
Solvent Detergent SDR HyperD
Removal
Bulk Sorbent
20033-065
20033-031
20033-023
20033-015
5 mL Bottle
25 mL Bottle
100 mL Bottle
1000 mL Bottle
"Mixed-mode" of size exclusion, normal
phase, and reversed phase. Composite
sorbent that combines a silica bead moiety
filled with long chain aliphatic polymers
that are cross-linked to provide a 3D mesh.
70 µm
60-80 mg/mL10
2-12
Solvent detergent removal with
excellent recovery of protein
AcroSep
Columns
SDR HyperD
20033-C001
1 mL Column, 5/pkg
Convenient pre-packed column for
detergent removal applications. Easily
attaches to syringe for quick processing.
70 µm
60-80 mg/mL10
2-12
Solvent detregent removal with
excellent recovery of protein
Hydroxyapatite
Bulk Sorbent
HA Ultrogel
24775-075
24775-082
24775-025
24775-017
24775-041
5 mL Bottle
25 mL Bottle
100 mL Bottle
500 mL Bottle
1000 mL Bottle
Composed of cross-linked agarose beads with
micro-crystals of hydroxyapatite entrapped in the
agarose mesh.
120 µm
Cytochrome C:
≥ 7 mg/mL12;
BSA: ≤ 7 mg/mL13
5-13
Fractionation, purification of biomolecules
Ultrogel AcA 44
Ultrogel AcA 54
Ultrogel AcA 202
Ion Exchange
Separation
by Charge
Bulk Sorbent
Acrodisc® Mustang Q
Chromatography Unit
Acrodisc Mustang S
Chromatography Unit
AcroSep
Columns
Mixed-Mode
Bulk Sorbent
AcroSep
Columns
Desalting capacity
45% gel volume
1 mL Column, 4/pkg
Strong cation exchange membrane in a 96-well
filter plate configuration.
Strong anion exchange membrane in a syringe
filter configuration.
Strong cation exchange membrane in a syringe
filter configuration.
Reduction of endotoxins in water, buffer, and certain biological solutions
1. Capacity determined using a 5 mg/mL BSA in 50 mM Tris-HCl, pH 8.5 at 2-minute residence time.
2. Capacity determined using 5 mg/mL hlgG in 50 mM sodium acetate, pH 4.5 at 2-minute residence time.
3. Dynamic binding capacity, 10% breakthrough, 200 cm/hr; sample: 5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6.
4. Dynamic binding capacity, 10% breakthrough, 200 cm/hr; sample: 5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5.
5. Dynamic binding capacity, 10% breakthrough, 200 cm/hr; sample: 5 mg/mL human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7.
6. Capacity determined using 5 mg/mL albumin in PBS buffer.
7. Dynamic binding capacity, 10% breakthrough, 100 cm/hr, determined using 10 mg/mL human IgG in PBS, pH 7.4; elution in 0.1 M sodium citrate, pH 2.5; column 4.6 ID x 100 mm.
8. Dynamic binding capacity at 600 cm/hr, using human ATIII at 72.5 UI/mL in 20 mM Tris-HCl, 0.3 M NaCl, pH 7.4, elution with 20 mM Tris-HCl, 2 M NaCl, pH 4.7, 10 cm bed height.
9. Dynamic binding capacity, 10% breakthrough; determined using 5 mg/mL human IgG in PBS, flow rate: 60 cm/hr.
10. Dynamic binding capacity, 10% breakthrough at 300 cm/hr, determined using 5 mg/mL Tritonu X-100 in PBS pH 7.4.
11. Dynamic binding capacity, 10% breakthrough; 100 cm/hr, 5 mg/mL BSA in PBS.
12. Determined using 5 mg/mL Cytochrome C diluted 50/50 in 10 mM sodium phosphate buffer, pH 6.8, at 30 cm/hr.
13. Determined using 1 mg/mL BSA diluted 50/50 in 10 mM sodium phosphate buffer, pH 6.8, at 12.5 cm/hr.
Visit us on the Web at www.pall.com/lab
E-mail us at LabSupport@pall.com
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
1.800.521.1520 USA and Canada
(+)800.PALL.LIFE Outside USA and Canada
1.734.665.0651 phone
1.734.913.6114 fax
International Offices
Pall Corporation has offices and plants throughout the world in: Argentina, Australia, Austria, Belgium, Brazil, Canada, China, France,
Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, New Zealand, Norway, Philippines, Poland, Russia, Singapore, South
Africa, Spain, Sweden, Switzerland, Taiwan, Thailand, United Kingdom, and Vietnam. Distributors in all major industrial areas of the world.
To locate the Pall office or distributor nearest you, visit www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be subject to change
without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2011, Pall Corporation. Pall,
, Acrodisc, AcroPrep, AcroSep, HyperCel, HyperD, Mustang, Trisacryl, and Ultrogel are trademarks
of Pall Corporation. ® indicates a trademark registered in the USA. Filtration.Separation.Solution. is a service mark of Pall Corporation.
uCibacron is a trademark of CIBA-GEIGY LTD. Triton is a trademark of Union Carbide.
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