University of Colorado at Colorado Springs
_________________________________________________________________________
Environmental Health and Safety
Department of Public Safety
1420 Austin Bluffs Parkway
Colorado Springs, Colorado 80918
719-255-3201
Animal Biosafety Level 2 (ABSL-2) Training Quiz
Directions: Complete this Quiz and e-mail it to cnorton@uccs.edu or mail it to DPS-207. In
order to receive credit for this training, you must provide the following contact information.
Name:
e-mail:
Lab PI:
Date:
Status (Undergrad, Grad, PostDoc, Faculty):
Phone #:
Department:
QUIZ SCORE:
Risk Assessment & Risk Management
1.
A Risk Assessment identifies risk factors associated with the work to be performed.
____ True ____ False
2.
What is Risk Management?
____ a) manages lab activities that can be hazardous
____ b) manages the animal procedures
____ c) manages the biological and chemical agents used in animal procedures
____ d) manages the risks identified from the risk assessment
3.
What are the common risk factors associated with the animal?
____ a) potential for bites and scratches, allergens, and animal facility
____ b) allergens and needle sticks
____ c) species, potential for bites and scratches, allergens, and zoonoses
____ d) species and the animal facility
4.
What are the common risk factors associated with the agent studied?
____ a) agent’s stability and pathogenicity, dose used, potential to infect humans, and the
manner and degree the animal sheds the agent
____ b) agent’s pathogenicity, potential to infect humans, and the manner and degree the
sheds the agent
____ c) agent’s stability and pathogenicity
____ d) how the agent is being introduced into the animal and the dose used
5.
What are the common risk factors associated with the research?
____ a) agent’s pathogenicity and dose used
____ b) what Risk Group the agent is in
____ c) agent’s concentration, procedures and/or equipment used, and the potential for
transmission to humans (i.e. spill, needle stick, aerosolize agent)
____ d) how the agent is being introduced into the animal and the dose used
animal
6.
The following factor(s) must also be taken into account when performing a risk assessment:
a)
b)
c)
d)
e)
7.
immunization is available for this agent
treatment is available if exposed to agent
health status of lab personnel (i.e. immunocompromised)
skill and expertise level of the lab personnel and animal care personnel performing the work
all of the above
A risk management plan must be specific to the agent(s) being used in describing the facility and
biosafety containment level, the safety equipment, and the personal protective equipment and clothing
required.
____ True ____ False
Working at Animal Biosafety Level 2 (ABSL-2)
8.
Work at ABSL-2 is suitable for work with animals infected with biological agents that are associated with
human or animal disease.
____ True ____ False
9.
For pathogens at ABSL-2, the risk of infection from inhalation of aerosols is typically greater than the risk
of infection through cuts or breaks in the skin, ingestion and splashes to the eyes, nose or mouth.
____ True ____ False
10.
The agents that typically fall into the ABSL-2 category are referred to as:
a) Risk Group 2 pathogens
b) Risk Group 3 pathogens
c) Risk Group 1 pathogens
d) Risk Group 4 pathogens
11.
The air supply and exhaust system maintains the following pressure within the animal room relative to the
adjoining spaces:
____ a) positive pressure
____ b) negative pressure
____ c) neutral pressure
12.
A hand washing sink is not required to be in the immediate area of performing work with infected
animals?
____ True ____ False
13.
Work being performed in animals with Risk Group 2 agents can be done in the animal facility or lab
where there is unrestricted access.
____ True ____ False
14.
At a minimum, the following protective clothing must be worn when working at ABSL-2:
a) gloves, lab coat, and other clothing to protect eyes, nose and mouth from splashes
b) gloves, lab coat, shoe coverings
c) lab coat, hair cover, surgical mask
d) face shield, lab coat, hair cover
15.
It is very important to conduct a risk assessment for each animal protocol to determine the appropriate
personal protective clothing and equipment.
____ True ____ False
16.
Individuals who have access to an ABSL-2 facility must have specialized training which is specific to the
hazards associated with the work being performed.
____ True ____ False
17.
If a risk assessment has been completed for the animal protocol, then no training is required prior to
entering an ABSL-2 facility or working with animals at ABSL-2.
____ True ____ False
18.
In order to properly disinfect all interior surfaces of the biosafety cabinet, it is acceptable to stick your
head inside the cabinet as long as the cabinet blower is running.
____ True ____ False
19.
A Class II biosafety cabinet or another physical containment device should always be used for aerosol
generating procedures involving infectious agents.
____ True ____ False
20.
Which of the following is an example of an aerosol generating procedure?
a) harvesting tissues or fluids
b) cage changing
c) necropsy
d) all of the above
21.
Whenever injecting an animal with an infectious agent, it is important to wipe the injection site with a
disinfectant after the injection to absorb any excess agent that may have been expelled by the animal
or needle onto the animal’s fur.
____ True ____ False
22.
Which of the following statements is correct for proper disinfection?
a) use a 10% bleach solution
b) use a disinfectant and sufficient contact time that is specific to the infectious organism(s)
c) use a disinfectant that is specific to the infectious organism(s)
d) use 70% ethanol solution
23.
Items in the biosafety cabinet which have not come in contact with the animal or the biological agent may
be removed from the cabinet without being decontaminated with an effective disinfectant for the
appropriate contact time.
____ True ____ False
24.
When exiting the animal room, in what order should protective clothing be removed?
a) shoe covers, then outer gloves, then lab coat, then inner gloves, then hair cover and
face protection last
b) outer gloves, then lab coat, then shoe covers, then hair cover and face protection, then
inner gloves last
c) outer gloves, then lab coat, then shoe covers, then inner gloves, and then hair cover
and face protection last
d) shoe covers, then lab coat, then outer gloves, then hair cover and face protection, and
inner gloves last
25.
The best way to avoid contaminating areas of your skin while removing protective clothing is to turn the
clothing and gloves inside-out as they are removed.
____ True ____ False