University of Colorado at Colorado Springs _________________________________________________________________________ Environmental Health and Safety Department of Public Safety 1420 Austin Bluffs Parkway Colorado Springs, Colorado 80918 719-255-3201 Animal Biosafety Level 1 (ABSL-1) Training Quiz Directions: Complete this Quiz and e-mail it to cnorton@uccs.edu or mail it to DPS-207. In order to receive credit for this training, you must provide the following contact information. Name: e-mail: Lab PI: Date: Status (Undergrad, Grad, PostDoc, Faculty): Phone #: Department: QUIZ SCORE: Risk Assessment & Risk Management 1. A Risk Assessment identifies risk factors associated with the work to be performed. ____ True ____ False 2. What is Risk Management? ____ a) manages lab activities that can be hazardous ____ b) manages the animal procedures ____ c) manages the biological and chemical agents used in animal procedures ____ d) manages the risks identified from the risk assessment 3. What are the common risk factors associated with the animal? ____ a) potential for bites and scratches, allergens, and animal facility ____ b) allergens and needle sticks ____ c) species, potential for bites and scratches, allergens, and zoonoses ____ d) species and the animal facility 4. What are the common risk factors associated with the agent studied? ____ a) agent’s stability and pathogenicity, dose used, potential to infect humans, and the manner and degree the animal sheds the agent ____ b) agent’s pathogenicity, potential to infect humans, and the manner and degree the sheds the agent ____ c) agent’s stability and pathogenicity ____ d) how the agent is being introduced into the animal and the dose used 5. What are the common risk factors associated with the research? ____ a) agent’s pathogenicity and dose used ____ b) what Risk Group the agent is in ____ c) agent’s concentration, procedures and/or equipment used, and the potential for transmission to humans (i.e. spill, needle stick, aerosolize agent) ____ d) how the agent is being introduced into the animal and the dose used animal 6. The following factor(s) must also be taken into account when performing a risk assessment: a) b) c) d) e) 7. immunization is available for this agent treatment is available if exposed to agent health status of lab personnel (i.e. immunocompromised) skill and expertise level of the lab personnel and animal care personnel performing the work all of the above A risk management plan must be specific to the agent(s) being used in describing the facility and biosafety containment level, the safety equipment, and the personal protective equipment and clothing required. ____ True ____ False Working at Animal Biosafety Level 1 (ABSL-1) 8. Work at ABSL-1 is suitable for work with animals infected with biological agents that are characterized and are not likely to cause disease in healthy workers or other animals. ____ True ____ False 9. The agents that typically fall into the ABSL-1 category are referred to as: a) Risk Group 2 pathogens b) Risk Group 3 pathogens c) Risk Group 1 pathogens d) Risk Group 4 pathogens 10. Work surfaces only need to be cleaned and sanitized after work is done. ____ True ____ False 11. The minimum level of protective clothing when working at ABSL-1 includes: a) gloves, safety glasses, lab coat b) gloves, lab coat, shoe coverings c) lab coat, hair cover, surgical mask d) face shield, lab coat, hair cover 12. A respirator may provide worker protection from animal allergens? ____ True ____ False 13. It is very important to conduct a risk assessment for each animal protocol to determine the appropriate personal protective clothing and equipment. ____ True ____ False 14. Personal protective clothing and equipment is not only used to protect the worker, but also to protect the animal. This is especially true if the animal is immunocompromised. ____ True ____ False 15. If a risk assessment has been completed for the animal protocol, then no training is required prior to entering an ABSL-1 facility or working with animals at ABSL-1. ____ True ____ False 16. All lab personnel may have access to an ABSL-1 facility or lab area where animal research is being performed at ABSL-1. ____ True ____ False 17. To control the spread of aerosols and allergens, the following can be used: a) a surgical mask and face shield b) ventilated cage racks or micro-isolator cages c) cages without bedding d) negative room pressure 18. Which one is not a special containment device used to minimize the release of dusts, allergens when opening cages and handling animals or when changing cages? a) biological safety cabinet b) locally exhausted downdraft cage dumping station c) trash can with lid d) negative pressure animal transfer station 19. Anyone handling animals must be trained in the specific handling and restraint procedures required to conduct your work and this training is species specific. ____ True ____ False 20. If you are bitten or scratched by an animal you should: a) immediately contact the faculty member in charge of the protocol b) immediately return to your lab c) call 911 d) immediately secure the animal, remove gloves, and wash the site for 15 minutes with soap and water 21. The use of sharps is restricted in an ABSL-1 facility. True False 22. Which of the following procedures will not help prevent a skin puncture or cut? a) keep sharps container within reach b) keep the non-dominant hand out of cutting area when using sharps c) dispose of sharps in a sharps container immediately after use d) change out sharps container when it becomes too full to close the lid 23. Animal carcasses can be placed in the trash as long as the bag is sealed? True False 24. Transporting animals out of the animal room may be done as long as a lid is on the cage. True False 25. After removing all protective clothing, the last thing to do before exiting the animal facility or lab is: a) clean all work surfaces with a disinfectant b) wash hands thoroughly c) remove trash from animal facility or lab d) turn off lights in room