An inter-species signaling system mediated by fusaric acid has parallel effects on antifungal metabolite production, gene expression, and antibiosis of Fusarium spp. by Pseudomonas protegens Pf-5
Maria Carolina Quecine, Teresa A. Kidarsa, Neal C. Goebel, Brenda T. Shaffer,
Marcella D. Henkels, T. Mark Zabriskie, and Joyce E. Loper
Table S1. Inhibition of Fusarium spp. by P. protegens Pf-5 and derivative strains on PDA containing 10
M FeCl
3
.
-4
Strain
Pf-5
ΔgacA
ΔphlD
ΔprnC
ΔrzxB
ΔhcnB
ΔpltA
ΔphlD ΔprnC
ΔphlD ΔrzxB
ΔprnC ΔrzxB
ΔphlD ΔrzxB ΔprnC
ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA
Percent inhibition
T4-0
41 ab
7 fgh
25 cde
R2-238 R5
35 bc
4
24 e d
37 a
0 e
24 abc
34 abc
26 cde
44
4 a
34 abc
24 cde h
16 efg
8 fgh
41 a
34 bc
36 ab
36 ab
36 ab 37
26 cd
27 cd
5 e a
32 ab
10 cd
32 ab
40 a 37
4 e a
4 de
5 gh
3 h
0
1 e e
0
0 e e ΔphlD ΔrzxB ΔprnC ΔhcnB ΔpltA ΔofaA ΔtoxB
Cultures were grown for 5 days at 27 o
C before radial growth of Fusarium spp. was measured. Means represent four replicate plates. Within each column, means followed by a common letter do not differ significantly as determined by Tukey’s range test.

50
40
30
20
10
0
Pf-5 Δ gacA Δ phlD Δ phlA Δ phlD Δ phlA Δ phlD Δ phlA Δ phlD
Δ rzxB Δ rzxB Δ rzxB Δ rzxB Δ rzxB
Δ prnC Δ prnC Δ prnC
Δ pltB
Δ hcnA
FIG. S1. Inhibition of Fusarium spp. by P. protegens Pf-5 and derivative strains on PDA amended with 10 -4 M FeCl
3
. Pf-5 and derivatives having deletions in specified genes are shown on the X axis. Percent inhibition of F. verticillioides isolate T4-0 (white bars), F. oxysporum f. sp. pisi race 2 isolate
R2-238 (grey bars), and F. oxysporum f. sp. pisi race 5 isolate R5 (black bars) was determined as shown in Fig. 1. Cultures were grown for 5 days at 27 o C before radial growth of Fusarium spp. was measured. Means represent four replicate plates and error bars represent the standard error of the mean.
Quecine et al. 2016.
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