BSC 2010- Exam3 (50 points total); 10/03/03 Name________KEY_________________ Social Security#_____________________ Honor Code Pledge- In taking this exam I have adhered to the Academic Honor Code of Florida State University. ____________________________(your signature) On the back of the last page of the exam list any questions on this exam which you feel were ambiguous, unclear or unfair and why. Instructions. Insert the following information in the box in the upper left had corner of the test form (see figure at right): Instructor- Ellington, Course- BSC 2010, Section01, form- exam # 3B, Date – 10/17. In the space marked “print last name first” write your name in block letters (last name then “space” first name) and bubble the appropriate letter in using a # 2 pencil. In the “special coding section”, B PRINT A LARGE “ ”. Put your social security number in the “identification no.” space and bubble numbers below. Failure to follow the above directions may lead to you getting no credit for the exam. 1 The exam consists of 25 multiple choice questions (answer sheet numbers 1-25) worth 2 pts each. You are to choose the most correct answer and bubble the corresponding letter in the answer sheet. The question will be marked wrong if you have more that one choice bubbled. If you erase an answer, make sure that you remove all marks. BEST OF LUCK ON THIS EXAM! 1. In catabolic pathways of cellular energy metabolism (like glycolysis and the Krebs cycle) which of the following is NOT TRUE- (A) molecules are broken down into smaller components, (B) entropy is increased, (C) the overall DG for the pathway is positive, (D) oxidation-reduction [redox] reactions are involved or (E) none of the above. 2. The ultimate source of electrons for non-cyclic photophosphorylation in photosynthesis is _________ . (A) CO2 breakdown, (B) oxygen, (C) FADH2, (D) water breakdown or (E) ATP 3. Acetyl CoA has _____ carbon(s) while pyruvate has _____ carbon(s). (A) 1, 2 2,2 (B) 3, 2 (C) (D) 3,6 or (E) 2, 3. 4. ATP synthase molecules in mitochondria and chloroplasts couple the downhill movement of _____ through a membrane system to the endergonic process of synthesis of __________. (A) sodium, NADH, (B) glucose, ATP, (C) protons, ATP, (D) water, ATP or (E) protons, NADH 5. Substrate level phosphorylations occur- (A) in glycolysis but not glycogenolysis, (B) in glycolysis but not the Krebs cycle, (C) in both glycolysis and the Krebs cycle, (D) in the Krebs cycle but not in glycolysis or (E) none of the above. For questions 6 and 7 consider the lactate dehydrogenase reaction: pyruvate + NADH + H à lactate + NAD+ 6. We can say that during the conversion of pyruvate to lactate, pyruvate ___________. – (A) has been oxidized, (B) has been reduced, (C) is the reducing agent, (D) is neither oxidized nor reduced or (E) none of the above. 7. In animals, the above reaction _______ (A) occurs under anaerobic conditions, (B) involves oxidation-reduction, (C) is vital to the oxidative phase of glycolysis [in the absence of oxygen], (D) all of the above or (E) none of the above. 8. Which of the following is (are) product(s) of the Krebs cycle that transfer electrons to the electron transport chain- (A) NADP+, (B) NADH and FADH2, (C) FAD+ and ATP, (D) GTP and NADH or (E) UTP and ATP. 9. The movement of an electron from a more electronegative molecule to a less electronegative molecule – (A) is endergonic, (B) has a + DG, (C) is non-spontaneous, (D) all of the above or (E) none of the above. 10. For every turn of the Krebs cycle _____ CO2 molecule(s) is (are) released and ______molecule(s) of FADH2 is(are) produced.- (A) 0, 2, (B) 1,1, (C) 2,0, (D) 2,1, (E) 2,2 2 11. In the electron transport chain the oxidation of NADH produces one more ATP than FADH2 oxidation because- (A) the DG for NADH oxidation to water is more negative than the DG for FADH2 oxidation to water, (B) NADH transfers its electrons at the beginning of the electron transport chain, (C) during NADH oxidation three H+’s are transported into the intermembrane space while only two are transported when FADH2 is oxidized, (D) all of the above or (E) none of the above. 12. Which is(are) required for the dark reactions of photosynthesis to take place?- (A) CO2, (B) ATP, (C) NADPH, (D) all of the above or (E) none of the above. 13. The net ATP production (per unit hexose [6 carbon sugar like glucose] degraded) of glyogenolysis is higher than that of glycolysis because- (A) there is one more oxidative step in glycogenolysis, (B) there is one more ATP yielding reaction in glycogenolysis, (C) pyruvate is not produced in glycogenolysis, (D) there is one less activation or “investment” reaction step in glycogenolysis or (E) there are no activation or “investment” reaction steps in glycogenolysis. 14. One of the major products of the triose phosphate dehydrogenase (also known as the 3-glyceraldehyde phosphate dehydrogenase) reaction in the oxidative step [phase] of glycolysis is_____.(A) ATP, (B) NAD+, (C) glucose-6-P, (D) ADP or (E) NADH. 15. In the complete oxidative catabolism of glucose yielding water and carbon dioxide, where do the electrons ultimately end up ? (that is, the last step in electron transport chain produces what?) (A) NADH, (B) FADH2, (C) pyruvate, (D) lactate or (E) water 16. Which of the following does NOT occur during the light reactions of photosynthesis(A) NADP+ reduction to NADPH, (B) production of oxygen, (C) phosphorylation of ADP, (D) absorption of light or (E) CO2 fixation. 17. The pyruvate dehydrogenase reaction _______. (A) takes place in mitochondria, (B) produces carbon dioxide, (C) produces acetyl-CoA, (D) produces NADH or (E) all of the above. 18. The role of Rubisco (RuBP carboxylase) is to (A) phosphorylate a five carbon sugar, (B) split water into oxygen, (C) use NADPH, (D) attach CO2 onto a five carbon sugar or (E) none of the above. 19. Photosystems I and II _________. (A) are the light harvesting units of the thylakoids, (B) are located ONLY in the plant mitochondria, (C) absorb all wavelengths of light, (D) are the sites of CO2 fixation or (E) are present in the stroma ONLY. 20. The stage of photosynthesis that actually produces sugar is called ______. (A) “Z” scheme, (B) cyclic photophosphorylation, (C) glycogen synthesis, (D) Calvin cycle or (E) non-cyclic photophosphorylation. 21. Which of the following is(are) true about primary electron acceptors in chloroplasts? (A) are proteins, (B) receive electrons from chlorophyll, (C) are part of both non-cyclic and cyclic electron flow, (D) participate in the light reactions of photosynthesis or (E) all of the above. 3 22. Where are protons are pumped during electron transport in chloroplasts? (A) cytosol, (B) matrix, (C) intermembrane space, (D) thylakoid space or (E) nucleus. 23. The process in which light causes an electron to jump to a higher shell is called- (A) water hydrolysis, (B) photophosphorylation, (C) fluorescence, (D) photobleaching or (E) photoexcitation. 24. The pyruvate kinase reaction (PEP + ADP à pyruvate + ATP) ________ . (A) is a good example of an endergonic reaction, (B) has a positive DG, (C) usually occurs in the cytosol, (D) usually occurs in the mitochondrion or (E) none of the above. (Hint: pyruvate kinase occurs in the energy yielding phase of glycolysis/glycogenolysis). 25. Which of the following is(are) true about the Calvin Cycle? (A) produces oxygen as a byproduct, (B) requires more NADPH than ATP for glucose synthesis, (C) requires an equal amount of NADPH and ATP for glucose synthesis, (D) requires three complete turns of the cycle to make a glucose molecule or (E) none of the above. Please check to see if you have filled out the bubble sheet completely (and correctly) 4