CSI – Web Adventure: Go to http://forensics.rice.edu/
Step 1: Register to play. This way if you do not finish, you can log back on and pick up where you left
off. You must follow these guidelines for registering
User ID: The initial of your first name, plus the first five letters of your last name. For example
my user id would be: bbarth
Password: wildcat (plus the last TWO numbers of your student ID number)
Click on: Case One - Rookie Training
Under Checklist, click on Forensic Biology
The goal is to earn your swabs at the end of this activity!!
Click on Follow Greg
Click on What is DNA? Then answer the questions below:
1.
What does DNA stand for?
2. Why is DNA called a blueprint?
3. Name the two places where DNA is located in a cell? (you may have to use the back button)
4. List the three correct answers you got from the DNA results from Sample A & B
a.
b.
c.
5. True or False – Identical twins can have the same type of DNA.
Click on What is a DNA Profile?
1.
What is a DNA profile?
2. What is the name of the largest database of DNA profiles? Who manages it?
3. Why do markers in a profile have different peaks?
4. How can markers be useful in comparing DNA samples?
5. A complete DNA profile will match only one person in __________________________.
Click on How do you process DNA?
1.
What are the four basic steps in DNA Processing?
a. ________________ - (explain)
b. ________________ - (explain)
c. ________________ - (explain)
d. ________________ - (explain)
2. How did you collect DNA sample from Greg?
3. What is a buccal swab?
4. Why are buccal swabs used?
5. Why must the cells and nuclei be lysed?
6. What technique is used to amplify the DNA?
7. What does PCR stand for? How many copies of DNA can be made from this process?
8. Why is it important to make many copies of DNA markers?
9. What is the first step in the PCR process?
10. How long does it take the PCR machine to replicate the markers?
11. After the markers are amplified, they need to be sorted by ______________.
12. The samples are moved from the PCR machine to the _________________
______________.
13. What does the Genetic Analyzer do?
14. How does this separate the markers based on size?
15. Describe the function of the laser and the sensor in the Genetic Analyzer:
16. The computer is able to show information gathered by the Genetic Analyzer. What do the
peaks show on the computer print-out?
17. How many separate markers are there?
After matching Greg’s DNA sample, click on the Exit Exam.
At the end of the exam, which suspect’s DNA matched? Mentally number the suspects 1, 2, 3, and 4
starting from the left.
Suspect No. ___________
Now go to the following website:
http://media.pearsoncmg.com/ph/chet/chet_saferstein_criminalistics_9/swf_files/9-1/DNACD_mod021-07_aud.swf
Draw a picture of the nucleotide shown. Be sure to identify the base, the sugar and phosphate group.
The backbone of DNA is made up of __________________ & _______________________________.
What are the four different bases found in DNA and what are their symbols?
List all the possible base pairs:
Describe the structure of a strand of DNA: