Name: Date: Biology 11: Domain Eukarya Purpose: Apply the Kingdom classification system to study a diversity of organisms Compare the characteristics of a prokaryotic cell with those of a eukaryotic cell Understand the main characteristics of Kingdom Protista Purpose: 330 and 337 *This is required reading! What are Eukaryotes? Introduction The domain system of classification is based on ___________________ differences, revealing that there are three different types of organisms. Which of the domains above have prokaryotic cells? __________________________________________ Which domain above has eukaryotic cells? _________________________________________________ Name the eukaryotic kingdoms: __________________________________________________________ _____________________________________________________________________________________ Biology 11: Domain Eukarya, Kingdom Protista Page 1 Prokaryotes vs. Eukaryotes What are the major differences between prokaryotic and eukaryotic cells? Character Plasma Membrane Prokaryotic Eukaryotic Cell Wall Nuclear Membrane Mitochondria Endoplasmic Reticulum Ribosomes What is one error with the diagram above? _____________________________________________________________________________________ Biology 11: Domain Eukarya, Kingdom Protista Page 2 Eukaryotic Cell Evolution Where do we think the endoplasmic reticulum and nuclear envelope came from? _____________________________________________________________________________________ Mitochondria and chloroplasts most likely came from the endosymbiosis of aerobic prokaryotes. What does this mean? _____________________________________________________________________________________ Biology 11: Domain Eukarya, Kingdom Protista Page 3 _____________________________________________________________________________________ Common Eukaryotic Life Cycles Which of the three types of life cycles has a diploid adult stage and only the gametes are haploid? _____________________________________________________________________________________ Which life cycle has a haploid adult and only the zygote is diploid? _____________________________________________________________________________________ Which type of life cycle has a haploid adult called a gametophyte and a diploid adult called the sporophyte? _____________________________________________________________________________________ Which life cycle is found in: a) Humans: ____________________ b) Protists and Fungi: ____________________ c) Plants: ____________________ Kingdom Protista Purpose: Protists are the most primitive form of eukayotic life and provide biologists with an opportunity to investigate how eukaryotic cells were first formed. Text Pages: 330 to 339 *This is required reading! Biology 11: Domain Eukarya, Kingdom Protista Page 4 Kingdom Protista is a “catch-all” grouping where biologists have put eukaryote organisms that do not easily fit into the other Animal Kingdoms. For this reason, the life forms found here are very diverse: Biology 11: Domain Eukarya, Kingdom Protista Page 5 Generally, protists can be defined as: _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ The great diversity of organisms found in the Kingdom Protista is organized based on two characteristics: Biology 11: Domain Eukarya, Kingdom Protista Page 6 a) Mode of Nutrition Autotrophic: ________________________________________________________ ________________________________________________________ Heterotrophic: ________________________________________________________ ________________________________________________________ ________________________________________________________ ________________________________________________________ b) Mode of Locomotion ________________________________________________________ ________________________________________________________ ________________________________________________________ *Watch Protist Movement video. Protist Diversity Biology 11: Domain Eukarya, Kingdom Protista Page 7 The great variety of protists we have today is a product of Secondary Endosymbiosis, the process through which an autotrophic protest becomes endosymbiotic in a heterotrophic protist: Below is a phylogenetic tree demonstrating a recent organization of protists into different groups: Biology 11: Domain Eukarya, Kingdom Protista Page 8 Chromalveolata This supergroup is controversial because it contains such a great diversity of protists a) Diatoms are autotrophic unicellular algae with a silica rich cell wall. They are key primary producers in fresh and salt water ecoystems. b) Dinoflagellates have two flagellae (one is free and the other is wrapped in a groove around the cell). They can be autotrophic, heterotrophic or mixotrophic and are important food sources. Dinoflagellates cause “red tide”. What is this and why is it important to know about on the Pacific Coast? __________________________________________________ __________________________________________________ __________________________________________________ __________________________________________________ __________________________________________________ __________________________________________________ c) Brown Algae are multicellular and get their brown colour from photosynthetic pigment. We know them as brown seaweeds and they create important ecosystems for marine organisms (kelp forests). Brown algae have a “holdfast” (not roots!) that attaches to rocks. They can also have air bladders that function in reproduction and to keep the algae blades close to the surface. Brown algae are used in sushi, cosmetics and as a stabilizer in ice cream. Biology 11: Domain Eukarya, Kingdom Protista Page 9 d) Water Molds are unicellular decomposers, although they can also be parasitize fish skin or gills and plants (Irish Potato Famine). e) Ciliates are heterotrophs and mixotrophs that use their cilia to move and sweep food into their mouths. Paramecium are a good example of ciliates and you will observe them in the Protist Lab. f) Plasmodium is a parasitic protist that is transferred to humans through mosquito bites and causes the disease Malaria. The protist feeds on red blood cells, causing them to die. *Watch Malaria: Human Host from HHMI Biology 11: Domain Eukarya, Kingdom Protista Page 10 Rhizaria and Unikonta Rhizaria and Unikonta are both controversial clades and they included together here as they both contain Amoebas. a) Amoebas are heterotrophic or parasitic (amoebic dysentery) and reproduce by binary fission. Amoebas move using pseudopodia, temporary extensions of the cell into which they push their cytoplasm. They engulf food material through phagocytosis. b) Plasmodial Slime Mold and Cellular Slime Mold are important decomposers. They also have interesting puzzle solving abilities and infrastructure planning potential! Biology 11: Domain Eukarya, Kingdom Protista Page 11 Archaeplastids Commonly known as algae, most Archaeplastids get their nutrients through ________________ and are important in aquatic ecosystems because they: __________________________________________________________ __________________________________________________________ a) Red Algae are autotrophic, often multicellular and gain their colour from accessory pigment that masks the green chlorophyll. Some Red Algae look like coral as they have calcium carbonate embedded in their cell walls. Red Algaes are used as nori in sushi, stabalizers ice cream and other products and to make Agar. b) Green Algae can be unicellular, multicellular or colonial and gain their colour from green chlorophyll. What are some characteristics that demonstrate Green Algae’s close relationship to land plants? _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Green Algae examples include: 1. Chlamydomonas (Unicellular) Reproduce sexually in favourable conditions and asexually in unfavourable. Follow the haplontic life cycle. Biology 11: Domain Eukarya, Kingdom Protista Page 12 2. Volvox (Colonial) Each volvox colony is a hollow ball of many algae cells and will eventually burst to release smaller daughter colonies. All cells in the colony of identical, but functionally individuals. 3. Ulva (Multicellular) Ulva is multicellular an exhibits cell specialization. This is very evident in its alternation of generation life cycle. Evolution of Multicellularity Biology 11: Domain Eukarya, Kingdom Protista Page 13 How are multicellular organisms different from unicellular organisms? _____________________________________________________________________________________ _____________________________________________________________________________________ Below is an image depicting how a unicellular protist may have eventually given rise to multicellular organisms. 1. An ancestral colony may have formed when a cell dividing and its offspring remain attached to one another. 2. Next, the cells in the colony may have become specialized and interdependent 3. Later on, additional specialization among the cells may have led to distinctions between sex cells (gametes) and non-reproductive cells (somatic cells). Multicellular organisms have evolved in three different lineages (Brown Algae, Fungi and Animals, Red and Green Algae). One theory suggests that 1 billion years ago two Unikot lineages divided to produce Fungi and Animals. Evidence of this division comes from molecular clocks and morphological similarities. Biology 11: Domain Eukarya, Kingdom Protista Page 14