Part 1 • Carbohydrates Energy Release energy is • When bonds are broken, __________ released for use by the cell ATP Glucose Fructose Sucrose: A disaccharide Monosaccharides Energy Storage • When bonds are made, energy (E) is stored Glucose: A Monosaccharide Fructose: A Monosaccharide Sucrose: A dissaccharide Carbohydrates 1. Carbohydrates are: – an important energy (E) source – Cellular structures 2. Carbon, Hydrogen and Oxygen in a ratio of 1:2:1 3. General Formula (CH CH2O)n O Carbon Water = hydrate Types of Carbohydrates Monosaccharides (simple sugars) – Contain 3-7 Carbons each • Examples: Glucose Glucose, Galactose, Fructose Types of Carbohydrates, cont… Disaccharides (two sugars) • Examples: Sucrose Sucrose, Maltose, Lactose – Maltose = Glucose + Glucose – Lactose = Glucose + Galactose Glucose Fructose Types of Carbohydrates, cont… Polysaccharides (many sugars) • Examples: Starch Starch, Glycogen, Cellulose Chloroplast Starch Liver Cell Cellulose Plant Cells Glycogen Plant Cells Check for Understanding… I’m a carbohydrate polymer made of 4 monomers. What are my monomers called? Monosaccharides, of course! Part 2 Bond Energy and Energy Storing Compounds ATP, NADPH, FADH2, NADH How is energy released? • When bonds are made by dehydration synthesis, energy is stored within the bonds of the compound. ATP OH HO Glucose Fructose Sucrose: A disaccharide Monosaccharides How is energy released? • When bonds are broken by hydrolysis, energy __________ is released from the bonds ATP Glucose Fructose Sucrose: A disaccharide Monosaccharides What are some examples of common energy storing compounds? 1. ATP (Most important usable energy for the cell.) 2. NADPH 3. FADH2 4. NADH How is ATP made? • ATP is made from the precursor AMP (Adenosine Mono-phosphate) • If a phophate and energy is added to AMP, ADP is created. • Furthermore, if another phosphate is added to ADP, ATP is created. How ATP is Made • AMP A P • ADP A P P • ATP A P P P Equation for ATP synthesis • ADP + P + energy ATP How are NADPH, FADH2, and NADH made? • • • • NADP+ + H+ + electrons NADPH FAD+ + 2H+ + electrons FADH2 NAD+ + H+ + electrons NADH Notice that high energy electrons and hydrogen ions (H+) are needed to create NADPH, FADH2 and NADH. Part 3 Introduction to Photosynthesis 1. Mesophyll 1. Mesophyll A layer of cells that contain chloroplasts & are responsible for most of the plant’s photosynthesis Page 2 Page 2 O2 2. Stomata Openings in plant leaves that allow for gas exchange to occur Carbon Dioxide (CO2) passes in and Oxygen (O2) passes out. CO2 Mesophyll Cell 3. Chloroplast The site of Photosynthesis Double-membrane bound organelle 4. Outer membrane 5. Inner membrane Page 2 8. Grana 7. Thylakoid Chlorophyll resides in these membranes 6. Stroma 8. Grana Page 2 Page 4 Pathway of Photosynthesis On your own, balance this equation: 6 CO2 + 6 H2O + (Light) 1 C6H12O6 + 6 O2 Reactants must equal Products Carbon 6 Carbon 6 Hydrogen 12 Hydrogen 12 Oxygen 18 Oxygen 18 Label the image in your notes, and fill in the notes provided Thylakoid Page 4 Page 4 Page 4 Photosynthesis: The Light Reaction • Chloroplasts are chemical factories powered by the sun. • Their thylakoids transform light energy into the energy of NADPH and ATP . Page 5 The Nature of Light Page 5 The Nature of Light • The particles of light are called photons . Page 5 Page 5 Why are leaves green? Substances that absorb light are called pigments Carotenoids Chlorophyll a Chlorophyll b Chlorophyll absorbs red and blue light, reflecting green Fluorescence of Chlorophyll High energy state e- Energy of electron Heat Photon Fluorescence Chlorophyll molecule Ground state Photosystems: Harvest Light Photon Electron Transfer (high energy state) Transfer of Energy Primary Electron Acceptor Reaction Center Chlorophyll Antenna pigment molecules Light Reaction Primary Acceptor Energy of Electrons Primary Acceptor 5) 2e- 2eETC NADP+ + H+ NADPH making enzyme 2) Spliiting of water releases O2 gas and refills 2e- to the chlorophill pigment H2O 2H 2e+ + O2 Electron Transport Chain (ETC) NADPH 3) Electrons “fall” in energy, moving through a protein complex called the ETC, and ATP is created from this energy 4) photons 1) photons 2 e- ATP Photosystem II Photosystem I Mechanical analogy for the light reactions 1. Photon excites an electron in Photosystem II 2. ATP is produced during this stage (ETC) 3. The electron moves on to Photosystem I 4. An electron is excited by another photon 5. NADPH is produced ATP Synthetase • An enzyme embedded in the thylakoid membrane • Creates ATP from the electron’s energy in the ETC Thylakoid membrane Protein Complex Enzyme Summary of the Light Reaction + + NADPH ++ + + 2e4e+ + + + + + + + + + + + + + + + + + + + + ATP Dark Reaction: An Overview • The General Formula for Photosynthesis is: 6CO2 + 6H2O + (Light ) C6H12O6 +6O2 • Which of these reactants has not been accounted for so far? ATP NADPH ATP & NADPH energy are used to convert CO2 into glucose CO2 Sugar! This is done in a three-phase cycle… RuBP PGA STEP 1: Carbon fixation 5-Carbon Sugar (RuBP) + CO2 two 3-Carbon Compounds (PGA) ATP and NADPH energy is used This energy rearranges atoms of PGA PGA changes into PGAL, a different 3-carbon sugar PGAL Phase 2: Makes PGAL, a 3Carbon sugar Phase 2: Makes PGAL, a 3Carbon sugar PGAL Let’s play the photosynthesis magnet board race!