1 THE BUILDING BLOCKS OF LIFE Mrs. Geist, Biology Swansboro High School, 2010-2011 Warm-Up 2 What does the term “organic” mean to you? Can you name foods rich in: carbohydrates/sugars lipids/fats, proteins? and Organic vs. Inorganic 3 Organic compounds: typically contain carbon (C) and are associated with life Inorganic compounds: usually do not contain carbon and are associated with non-living things Carbon is the building block of all living things!! Carbon 4 Has 4 electrons in its outermost shell 4 electrons to share Forms covalent bonds (shares electrons) Single, double, or triple bonds Atoms of nitrogen (N), oxygen (O), and phosphorus (P) attach to carbon chains. Macromolecules—giant molecules 5 4 major classes: Carbohydrates Lipids Proteins Nucleic acids Polymers- complex molecules Monomers- small molecules, single units Carbohydrates 6 Made of carbon (C), hydrogen (H), and oxygen (O) Ex: sugars and starches 2 H: 1 O Major source of energy Carbohydrates: Simple Sugars 7 Simple sugars (C6H12O6) smallest carbs. A.k.a. monosaccharides Ex: glucose, fructose, galactose Cell’s main source of energy Carbohydrates: Disaccharides 8 Disaccharide (C12H22O11)- two monosaccharides together Ex: sucrose, maltose, lactose Carbohydrates (cont’d.) 9 Polysaccharides- many monosaccharides 4 major classes of polysaccharides: 1. 2. 3. Starch- plant storage of glucose Glycogen- animal storage of glucose Cellulose- plants use for structural support 4. Most abundant organic molecule on Earth Indigestible bulk (or “fiber”) Chitin- animals use for support, insect skeletons 2nd most abundant organic molecule on Earth Warm-Up 8/31 10 In your own words, what is an organic macromolecule? What do the terms saturated and unsaturated fat mean to you? Where might we find protein in the body? Lipids 11 Made up of carbon (C), hydrogen (H), and oxygen (O) Functions: Store energy Provide insulation Important parts of cell membranes (phospholipids) Waterproof coverings (waxes, ex: bird feathers) Not soluble in water Ex: fats, oils, waxes, steroids, phospholipids Lipids (cont’d.) 12 Structure: 3 fatty acids Long chains of C with H attached 1 glycerol Alcohol with a hydroxyl (-OH) group on each of its 3 C atoms Dehydration synthesis: attaches these parts Removal of water (H2O) Lipids: Saturated vs. Unsaturated 13 Saturated Unsaturated Solid at room temperature Liquid at room temperature Contains lots of H Contains less H Single bonds between C atoms 1+ double bonds between C atoms Proteins 14 Made up of nitrogen (N), carbon (C), hydrogen (H), and oxygen (O) Monomer: amino acids Structure: Amino group (-NH2) on 1 end Carboxyl group (-COOH) on the other end Side chain (or R group) that differs for each of the 20 amino acids Proteins: Amino Acids 15 Structure of an amino acid (monomer) Proteins: 4 levels of organization 16 1. 2. Primary: sequence of amino acids Secondary: the amino acids within a chain can be twisted or folded 3. 4. Ex: alpha helices, beta pleated sheets Tertiary: the chain itself is folded Quaternary: If the protein has >1 chain, each chain has a specific arrangement in space Protein Functions 17 Some proteins control the rate of reactions and regulate cell processes (called enzymes). Act as catalysts- speed up chemical reactions some form bones, muscles, skin, and ligaments. Others transport substances into/out of cells Help to fight disease. Enyzmes as catalysts Enzymes are proteins that act as biological catalysts. Catalyst- speeds up the rate of a chemical reaction. Speed up reactions that are too slow or have activation energies that are too high to make them practical for living tissue. Enzymes act by lowering the activation energy. 19 Functions of Enzymes Regulating chemical pathways. Making material that cells need. Releasing energy. Transferring information. Enyzmes as catalysts For a chemical reaction to take place, the reactants must collide with enough energy to break existing bonds and form new bonds If reactants do not have enough energy, no reaction will take place. Enzymes Enzymes are very specific, generally catalyzing only one chemical reaction. For this reason, part of an enzyme’s name is usually derived from the reaction it catalyzes. Add –ase ending. I.e. lipase I.e. proteinase The Enzyme-Substrate Complex Enzymes provide a site where reactants can be brought together to react, reducing the energy needed for reaction. The reactants are known as substrates. The enzyme and substrates remain bound together until the reaction is done and the substrates are converted to products. The products of the reaction are released and the enzyme is free to start the process again. The cell theory states that new cells are produced from nonliving material. existing cells. cytoplasm. animals. Copyright Pearson Prentice Hall The person who first used the term cell was a. Matthias Schleiden. b. Lynn Margulis. c. Anton van Leeuwenhoek. d. Robert Hooke. Copyright Pearson Prentice Hall An Enzyme-Catalyzed Reaction Substrates: glucose and ATP Enzyme: hexokinase Substrates bind to enzyme, forming an enzymesubstrate complex. The fit is so precise that the active site and substrates are often compared to a lock and key. Regulation of Enzyme Activity Enzymes work best at certain pH and temperature ranges and can be affected by such changes. Many enzymes are affected by changes in temperature. Cells can regulate the activities of enzymes. Most cells contain proteins that help to turn key enzymes “on” and “off” at critical stages in the life of the cell. Nucleic Acids Function: store and transmit hereditary, or genetic, information. 2 kinds of nucleic acids: 1. ribonucleic acid (RNA) 2. deoxyribonucleic acid (DNA). Nucleic Acids 29 Monomer = nucleotides, which are composed of 3 parts: 5-carbon sugar- deoxyribose (DNA) or ribose (RNA) Nitrogen base- adenine (A), thymine (T), cytosine (C), guanine (G), uracil (U) Phosphate group- contains phosphorus (P) and oxygen (O) 2 Types: Deoxyribonucleic Acid (DNA) Ribonucleic Acid (RNA): Deoxyribonucleic Acid (DNA) 30 Double helix (spiral) Stores genetic information Ribonucleic Acid (RNA) 31 single helix Plays a role in manufacture of proteins Enzyme (speed up reactions) Nucleotides 32 DNA and RNA Adenine (A) Guanine (G) Cytosine (C) DNA only Thymine (T) RNA only Uracil (U) 3 parts of a nucleotide 33 5-carbon sugar (deoxyribose in DNA or ribose in RNA) Nucleotide base Phosphate group The DNA and RNA Strand 5' Base Nucleotide + Nucleotide(n) 5' 1' 3' = DNA (or RNA) strand Base Base 3' 2005 VisiScience Corporation. All Rights Reserved. Does temperature affect an enzyme reaction? (pp. 164-165) Problem: Does the enzyme peroxidase work in cold temperatures? Does peroxidase work better at higher temperatures? After being frozen or boiled? Hypothesis: “If…, then …” statement. Materials: clock, beakers, kitchen knife, tongs, potato, ice, hot plates, thermometers, 3% hydrogen peroxide (H2O2), hot gloves 35 Peroxidase 2H2O2 2H2O + O2 Hydrogen Peroxide water + oxygen gas Hydrogen peroxide is damaging to cells Peroxidase speeds up the breakdown of hydrogen peroxide 36 Planning Experiment Hypothesis Boiling, ice bath, warm water bath, room temperature Steps to be taken Add 1 drop H2O2 to the potato slice and observe what happens What data will you collect? How will you record them? What factors should be controlled? How will you achieve those temperatures? Carry out the expt. 37