Journal 12/17 Procedure: 1. Turn in assignments to bin 2. Get out journal sheet 3. Get out notes on passive transport 4. Begin journal Quiet while you do this! Assignments Due: • Active transport HW DUE Today • Exam studyguide DUE tomorrow 12/18 • Exam: Homeostasis and transport 12/18 In 25 words, 1. Describe the 5 ways cells can transport materials across the plasma membrane. 2. Classify each as active or passive transport Journal 12/18 Procedure: 1. Turn in assignments to bin 2. Get out journal sheet 3. Get out notes on passive transport 4. Begin journal Quiet while you do this! Assignments Due: • Exam studyguide DUE today • Exam: Homeostasis and transport TODAY In 25 words, 1. Differentiate between osmosis, simple diffusion, and facilitated diffusion. 2. Differentiate between protein pumps and vesicle transport. Journal 12/14 Procedure: 1. Turn in assignments to bin 2. Get out journal sheet 3. Get out notes on passive transport 4. Begin journal Quiet while you do this! Assignments Due: • Active transport HW DUE Monday • Exam: Homeostasis and transport 12/18 In 25 words, 1. What are the two types of active transport? 2. Describe each type. Journal 12/13 Procedure: 1. Turn in assignments to bin 2. Get out journal sheet 3. Get out notes on passive transport 4. Begin journal Quiet while you do this! Assignments Due: • Passive transport review skill sheet DUE TODAY • Exam: Homeostasis and transport 12/18 In 25 words, 1. How is facilitated diffusion different from simple diffusion? 2. How is osmosis different from simple diffusion? Journal 12/12 Procedure: 1. Turn in assignments to bin 2. Get out journal sheet 3. Get out notes on passive transport 4. Begin journal Quiet while you do this! Assignments Due: • Stem cell research paper DUE TODAY! • Passive transport review skill sheet DUE Thurs. 12/13 (you did this with the sub yesterday) Quiz: Passive Transport 12/13 (Thurs) In 25 words, 1. What are the three types of passive transport? 2. Give a description of each. Journal 12/11 Week 16 Procedure: 1. Turn in assignments to bin 2. Get make-up work from bin 3. Get out journal sheet 4. Get out notes on passive transport 5. Begin journal Quiet while you do this! Assignments Due: • Stem cell research paper DUE 12/12 (Wed) • Passive transport review skill sheet DUE Thurs. 12/13 (you did this with the sub yesterday) Quiz: Passive Transport 12/13 (Thurs) In 25 words, 1. What is the difference between passive and active transport? 2. Define osmosis. 3. What is facilitated diffusion? Journal 12/7 Assignments Due: • Stem cell research paper DUE 12/12 (Wed) • Cell organelle remediation packet DUE TODAY Quiz: Passive Transport 12/13 (Thurs) In 25 words, 1. Give a brief description of what happened yesterday in Osmosis Jones. 2. What type of cell is Osmosis Jones? Agenda: We are watching Osmosis Jones! Journal 12/6 Assignments Due: • Stem cell research paper DUE 12/12 (Wed) • Cell organelle remediation packet DUE 12/7 (Fri) • Passive transport reading DUE 12/6 TODAY • Quiz: Passive Transport 12/13 (Thurs) In 25 words, 1. What is osmosis? 2. What is the difference between hypertonic, hypotonic, and isotonic? Agenda: We are watching Osmosis Jones! Journal 12/5 Assignments Due: • Stem cell research paper DUE 12/12 (Wed) • Cell organelle remediation packet DUE 12/7 (Fri) • Passive transport reading DUE 12/6 (Thurs) In 25 words, 1.What is the difference between passive and active transport? 2. What drives diffusion? Agenda 1. Finish notes 2. Short video review of passive transport Journal 12/4 Assignments Due: • Stem cell research paper DUE 12/12 (Wed) • Cell organelle remediation packet DUE 12/7 (Fri) • Passive transport reading DUE 12/6 (Thus) In 25 words, 1.What organelle controls what substances enter and leave the cell? 2.What materials make up the plasma membrane? Chapter 5 Homeostasis & Cell Transport VIDEO lab VIDEO Here a White Blood Cell eats some bacteria. How does the bacteria get into the cell? http://www.youtube.com/watch?v=fpOxgAU5fFQ 5-1 Passive Transport Objectives Explain how an equilibrium is established as a result of diffusion Distinguish between diffusion and osmosis Explain how substances cross the cell membrane through facilitated diffusion Explain how ion channels assist the diffusion of ions across the cell membrane Homeostasis The ability or tendency of an organism or cell to maintain internal equilibrium by adjusting its physiological processes Cell membranes help organisms maintain homeostasis by controlling what substances may enter or leave cells Why cells need to maintain homeostasis: Cells need to be able to be different than their environment Maintaining pH, water concentration, types of molecules, etc. Structure of cell membrane: Quiz Question!! What three macromolecules make up a cell membrane? • Phospholipids • Proteins • Carbohydrates The Cell Membrane is SEMIPERMEABLE ONLY ALLOWS CERTAIN MOLECULES TO PASS THROUGH Small molecules like WATER, OXYGEN, AND CARBON DIOXIDE can move in and out freely Large molecules like PROTEINS & CARBOHYDRATES CANNOT What is Equilibrium? A state that exists when the concentration of a substance is the same throughout a space A condition in which all acting influences are canceled by others, resulting in a stable, balanced, or unchanging system Reached when molecules are of the same concentration, though they keep moving 5 basic ways things get into or out of cells 1. 2. 3. 4. 5. Simple Diffusion Osmosis Facilitated Diffusion Cell Membrane Pumps Vesicles passive transport(1-3) active transport (4-5) Passive Transport Some substances can cross the cell membrane without any input of Energy by the cell. The movement of such substances across the membrane is known as PASSIVE TRANSPORT 1. Simple Diffusion 2. Osmosis 3. Facilitated Diffusion 1. Simple Diffusion • The movement of molecules from an area of high concentration to an area of low concentration CONCENTRATION GRADIENT -The difference in concentrations of molecules across a space Kinetic Energy of molecules Kinetic energy is energy of motion Molecules are in Constant Motion Molecules move in a straight line unless they hit something If no object blocks their path they keep moving-so- they move to where there are less molecules Diffusion occurs when molecules move randomly away from each other Diffusion is driven entirely by kinetic energy Simple Diffusion across a membrane Few substances can diffuse directly through the lipid bilayer of the membrane The only substances that can do this are lipidsoluble molecules (steroids, -or very small molecules, such as H2O, O2 and CO2) Since no energy is added -substances can only move down their concentration gradient 2. Osmosis -the movement of water molecules from an area of high concentration to an area of low concentration. Osmosis the diffusion of water across a membrane Same as regular diffusion, but water is so important it has its own name - osmosis. Water molecules can diffuse freely across a membrane, but always down a concentration gradient – so water diffuses from a dilute to a concentrated solution video rap Types of solutions There are three possible concentrations of solution to consider: Isotonic solution a solution of equal concentration of solutes to a cell Hypertonic solution a solution of higher concentration of solutes than a cell Hypotonic solution a solution of lower concentration of solutes than a cell Water moves freely across the cell membrane Note differences in “normal” state for plant/animal cells The more concentrated solution is Hypertonic compared to the solution with less solutes. The water will always try to rush in to make the more concentrated solution less hypertonic The less concentrated solution is referred to as Hypotonic, water will attempt to leave this compartment & decrease concentration of the solution with more solute When two compartments are equally concentrated they are Isotonic with respect to each other,& there’s no net diffusion of water http://www.youtube.com/watch?v= eQsAzXr0UCU osmosis demo Examples of problems that living cells face all the time Simple animal cells (paramecium) in fresh water habitats are surrounded by a hypotonic solution and constantly need to expel water using contractile vacuoles to prevent swelling & lysis Cells in marine environments are surrounded by a hypertonic solution, and must actively pump ions into their cells to reduce their water potential and so reduce water loss by osmosis Marine & Freshwater fish-homeostasis response: Freshwater fish- pump water out to keep from exploding. Marine fish-take in ions (salts) to keep all the water from leaving their body Plant Example Plant roots may be in a hypotonic environment. Since more water/ less solute is outside roots cells, water rushes into the plant cells (by osmosis). Cells swell against the cell wall. TURGOR PRESSURE is the pressure that water molecules exert against the cell wall Force gives the plant rigidity, and provides structural support You have probably experienced this with lettuce and celery that have been in the fridge too long. They are wilted and no longer crisp because they have lost their Turgor Pressure Red Onion Skin (a) Hypotonic - cells are pressed against cell walls (b) Hypertonic - cells contract and pull away from cell walls 3.Facilitated Diffusion -The transport of substances across a membrane by a trans-membrane protein molecule -No energy is involved -substances can only move down their concentration gradient 4 Steps Molecule binds to carrier protein Carrier protein changes shape Molecule released on other side of membrane Carrier protein returns to original shape 2 kinds of pump proteins Carrier Proteins have a binding site for a specific solute and constantly flip between two states so that the site is alternately open to opposite sides of the membrane The substance will bind on the side where it is at a high concentration and be released where it is at a low concentration ION Channel Proteins form a water-filled pore or channel in the membrane This allows charged substances (usually ions) to diffuse across membranes. (Most channels can be gated (opened or closed), allowing the cell to control the entry and exit of ions.) review video Exit Slip 1. How are diffusion and facilitated diffusion similar? How are they different? 5-2 Active Transport Objectives Distinguish between passive and active transport Explain how the sodium-potassium pump operates Compare endocytosis and exocytosis Active Transport -when cells must move materials up their concentration gradient, from an area of lower to an area of higher concentration 2 ways: 1. Cell Membrane pumps -Sodium-potassium pump 2. Vesicles - Macromolecule exchange - Endocytosis - Exocytosis 1. Active Transport (pumping) •Pumping of substances across a membrane by a trans-membrane protein pump molecule •The protein binds a molecule of the substance to be transported on one side of the membrane, changes shape, and releases it on the other side. • REQUIRES ENERGY: An active process • Use ATP -into ADP + P- use the energy released to change shape and pump the molecule The Na+K+ Pump -This transport protein is present in the cell membranes of all animal cells and is the most abundant and important of all membrane pumps 3 Sodiums (Na) out for every 2 Potassiums (K) in Exchange of 3 NA for 2 K -creates an electrical gradient across the cell membrane The outside of the membrane becomes positively charged (+) & the inside negatively (-) charged -Important for understanding electrical impulses like in nerve cells 2. Vesicles The processes described so far only apply to small molecules Large molecules (such as proteins, Endocytosis & Exocytosis- both polysaccharides and nucleotides) and even whole cells are moved in and out of cells by using membrane vesicles energy- so are active transports require Endocytosis- the process by which cells ingest external fluid, macromolecules & large particles Endocytosis Materials are enclosed by a fold of the cell membrane, which then pinches shut to form a closed vesicle When the materials and the vesicles are small (such as a protein molecule) the process is known as pinocytosis (cell drinking) If the materials are large (such as a white blood cell ingesting a bacterial cell) the process is known as phagocytosis (cell eating) Endocytosis Pseudopodia of a phagocytic cell Exocytosis Essentially endocytosis in reverse Vesicles in the cytoplasm fuse with the cell membrane & empty their contents outside the cell Summary of Membrane Transport uses energy uses proteins specific controllable Simple Diffusion N N N N Osmosis N N Y N Facilitated Diffusion N Y Y Y Active Transport Y Y Y Y Vesicles Y N Y Y method Membrane transport Small molecules passive transport simple diffusion active transport facilitated diffusion water channels nonpolar permeases ions pumps cootransporters Large molecules endocytosis exocytosis pinocytosis ENDOCYTOSIS EXOCYTOSIS