Used plasmid DNA, [pDNA] = 0.2ug/uL
Made two 1% Agarose Gel: 1 with EtBr & 1
without EtBr
 Prepared 6 samples of pDNA to be loaded in
each gel with different amounts of pDNA :
0.01ug, 0.05ug, 0.1ug, 0.5ug, 1.0ug & 5.0ug
 In gel without EtBr, added 1uL of EZ-Vision to
the 6 samples and the 1kb DNA ruler before
loading
 Ran gels at 120V for 1.5-2 hours
 Imaged them


5.0ug
1.0ug
0.5ug
0.1ug
0.05ug
0.01ug
1 kb DNA ruler
5.0ug
1.0ug
0.5ug
0.1ug
0.05ug
0.01ug
1 kb DNA ruler
EtBr Gel
EZ-Vision Gel



EZ-Vision fluorescent dye can be used as a
safer alternative to visualize DNA bands in an
agarose gel
EtBr is more sensitive at detecting DNA at
lower concentrations (<0.1ug) when
compared to EZ-Vision
EZ-Vision gel could be used on the UV
transilluminator to cut bands – tested and
found that you can detect the bands just as
clearly as if they were stained with EtBr.





Prepare samples and DNA ruler to be loaded
on gel in usual method. Add 1uL of EZ-Vision
dye to each before loading.
Mix sample to get homogenous solution.
Load and run gel.
Image gel. Remember to switch emission
filter to SybrGreen before using imaging
program.
Capture photo, save and/or print. DONE!