Most of this structure is only visible under the electron microscope To look at the structure of the cell and to consider the function of the parts. To discuss how a cell is organised and relate this to the processes carried out By the end of today I expect you to be familiar with the parts of the cell By the end of the week I expect you to know the functions of the main parts of the cell. 1. Cytoskeleton 2. Nucleus and ribosomes; 3. The endomembrane system; 4. Peroxisomes and vacuoles; 5. Energy related organelles; Create energy reproduce+ Constitute tissues Die Protect us Move Produce/ secrete Consume/ absorb These are called prokaryotes; Bacteria are prokaryotes; They are much smaller; They are much simpler – have much less ultrastructure. Genetic material not enveloped in nucleus No internal membrane structure based on nuclear envelope Cell not compartmentalised No plastids Cell membrane may be ensheathed in cell wall. May have flagellae and thus moves Much smaller than eukaryote All multicellular and most unicellular organisms that you will learn about; Larger and more complex Compartmentalised with different parts of the cell having different functions e.g. the nucleus; Certain organelles such as mitochondria and chloroplasts (plastids)may have evolved from the inclusion of prokaryotic cells in ancestral eukaryotes. They have own DNA and function fairly independently. Nucleus communicates with ribosomes Organelles of the membrane system communicate with each other; Energy related organelles are self-sufficient; Each organelle has its own enzymes and makes its own products; Products transported through cytoplasm in transport vesicles (little membranous sacs that keep them separate from the cytoplasm) Chemicals imported and exported across cell membrane in different ways. 600g x 10 minutes = nuclei; 15000g x 5 minutes = mitochondria and lysosomes; 100000g x 60minutes = ribosomes and endoplasmic reticulum; What remains in the solution is the soluble part of the cytoplasm A net of protein fibres that give structure to the cell and help in the movement of organelles: Protein fibres act as tracks for the transport vesicles; Without it the cell could not function because the chemicals could not be transported to where they are needed. Includes the centrioles which have a role in cell division. Nucleus contains chromatin in a semiliquid called nucleoplasm; The chromatin is fibres of DNA that form the chromosomes; Also contains RNA Ribosomes are made here. Different types of RNA and ribosomes involved in the transfer of information from DNA and protein synthesis Surrounded by a double membrane called a nuclear envelope which is full of nuclear pores through which substances enter and leave the nucleus; Ribosomes are made of a unit of RNA and a unit of protein They are where proteins are made. They can move around the cell and group together as polyribosomes or attach to the rough endoplasmic reticulum; The proteins they synthesise are coded for in the DNA Enzyme producing cells such as those in the pancreas contain many ribosomes because……. Consists of: The nuclear envelope; Smooth and rough endoplasmic reticulum (SER) and (RER); The Golgi apparatus’ Several types of vesicles Job is to synthesis and transport materials around the cell; To secrete chemicals from the cell, e.g. hormones; To recycle and destroy cellular components; Cell death The difference is whether they have ribosomes attached. RER contains ribosomes SER does not As a result they make different things. RER is where proteins are synthesised by the ribosomes SER is where lipids and steroids are synthesised, e.g. in cells in the testes it is where testosterone is made. Both form vesicles that transport materials to other parts of the cell – especially the Golgi body It alters and synthesises new chemicals from those delivered to it in vesicles from the RER and SER. It receives chemicals in vesicles from the ER on the inner face It packages chemicals into vesicles and releases them from the outer face. These vesicles may either be lysosomes or may deliver chemicals to the cell membrane for secretion Membrane bound vesicles produced by the Golgi apparatus Have a low pH Contain powerful hydrolytic digestive enzymes Recycle cell material Destroy non-functioning organelles In white blood cells help destroy engulfed bacteria and viruses. Have a role in programmed cell death Membranous sacs in the cell that have nothing to do with the endomembrane system. Peroxisomes contain enzymes often involved in the break down of materials such as lipids; Contain enzymes whose action result in the production of hydrogen peroxide which must then be broken down; Vacuoles store substances especially in plant cells where they contain sap and pigments and maintain plant structure by hydrostatic pressure; Collect excretory products of plants. In some organisms actively remove excess water. Self-sustained organelles (Plastids) Have their own DNA Mitochondria make ATP which stores the energy from aerobic respiration Chloroplasts contain pigment chlorophyll They are where photosynthesis occurs. The folded cristae of the mitochondria and stacked thylakoids of the chloroplasts increase the surface area for reactions. Evolutionary genesis is believed to be through the inclusion of prokaryotic cells. Often called the power house of the cell. Have an inner membrane folded into cristae and an outer membrane. Inner membrane encloses the matrix which is full of mitochondrial DNA and ribosomes; Matrix is a highly concentrated mixture of enzymes that break down carbohydrates and other nutrients. Supplies energy that allows the synthesis of ATP on the inner membrane. ATP is a chemical store of energy Bound by a double membrane Contain their own DNA Reproduce themselves Double membrane encloses a space called the stroma which contains discs made from a third membrane called thylakoids; A Stack of thylakoids is called a granum; Chlorophyl in the thylakoid membrane traps the sun’s energy which is used by enzymes in the stroma to create carbohydrates. Chromoplasts contain the colours in fruit, flowers & vegetables such as carrots and beetroot; Leucoplasts synthesise starches and oils Under the microscope it is possible to see many leucoplasts in starch rich vegetables such as potatoes. Plasma membrane, Mitochondrion, Chloroplast; Cytoplasm; Smooth endoplasmic reticulum; Rough endoplasmic reticulum; Golgi body; Ribosomes; Nucleus; Nucleolus; Nuclear Pore; Nuclear envelope; Chromatin; Centrioles; Lysosomes; Vacuoles; cristae in the mitochondria; matrix in mitochondria; In the chloroplast: stroma, thylakoids, granum and lumen Each person needs to find out the function of one of these and help everyone add it to their list. I will check. Quiz next week on the functions of the parts. http://www.cellsalive.com/cells/3dcell.htm http://www.sheppardsoftware.com/healt h/anatomy/cell/index.htm http://www.williamsclass.com/SeventhSc ienceWork/CellBrochure.htm (homework) Next lesson we are going to make a model of the cell. You must bring what you need to make it including scissors, tape and glue. It needs labeling Pictures of these are widely available on the net. You must include all the organelles I named You may make separate models of some parts to show detail Marks Model Explanation Innovation/presentati on 2 Some effort made Unable to explain Can’t tell what it is 4 A cell completed Explanation does not use technical terms Some organelles can be identified 6 Most organelles shown Some effort made to correctly name organelles Mostly fairly clear 8 Up to 3 organelles missing Most organelles correctly named Model shows different organelles clearly 10 All organelles shown 2 or fewer organelles Model makes not correctly named innovative use of materials to differentiate organelles Plasma membrane, Mitochondrion, Chloroplast; Cytoplasm; Smooth endoplasmic reticulum; Rough endoplasmic reticulum; Golgi body; Ribosomes; Nucleus; Nucleolus; Nuclear Pore; Nuclear envelope; Chromatin; Centrioles; Lysosomes; Vacuoles; cristae in the mitochondria; matrix in mitochondria; In the chloroplast: stroma, thylakoids, granum and lumen 1 2 3 4 5 • Write article for Ezine on your organelle • Include description of function and processes • Include images • Find a link to describe some science about it. • Find a link to video or cartoon that describes its action Marks Description processes Article 1 Just identified Just identified Just a link 2 Some facts Some aspects correct 3 Description mostly correct Description mostly correct 4 Description complete Description complete You´ve added a caption 5 Description of organelles complete and illustrated Description Brief clear of processes explanation complete and of content illustrated Caption comprehensi vely describes in brief what it´s about Brief explanation confused Video Just a link A good choice