Industrial Oil Seed Opportunities Linnaeus Plant Sciences Inc. Jack Grushcow www.linnaeus.net LINNAEUS Plant Sciences Current Key Factors • • • • • • • Cost and availability of Petroleum Environment Entrenchment Bio-tech Advances Patents Regulatory Canadian Infrastructure LINNAEUS Plant Sciences We need to look forward… to new industrial chemistry LINNAEUS Plant Sciences Overview • About Castor Oil • Core Technology • Lubricant markets and economics • Marketing Strategy • Regulatory Strategy LINNAEUS Plant Sciences Castor Oil LINNAEUS Plant Sciences LINNAEUS Plant Sciences Industrial Applications of Castor Oil • Over 1,000 patented industrial applications • Industrial chemistry established since 1900’s • Long recognized for outstanding properties including oxidative stability and lubricity • US Strategic stockpile up to ’70’s • Only commercial source of HFA LINNAEUS Plant Sciences Ricinoleic Acid Ricinoleic acid: a reactive fatty acid capable of numerous oleochemical conversions LINNAEUS Plant Sciences Problems with Castor • Contains Ricin potent bio-terror weapon • Main producers are India, China and Brazil • India and others seek down stream capability • Supply limited and variable • Significant price fluctuations • Largest user seeks alternative source LINNAEUS Plant Sciences Linnaeus Plant Sciences Hydroxy Fatty Acid (HFA) Technology Hydroxylase Gene Expression in Model Expression in Conventional Oilseeds Ricinus communis (Castor) Arabidopsis thaliana LINNAEUS Plant Sciences Advantages of (GM) Approach Conventional Approach Conventional Oils Industrial Oils Canola, Soybean, Corn Lesquerella (Castor) Vernonia Goals •High oleic •Low saturates •Low polyunsaturates •Limited profiles Goals •Agronomics •Infrastructure •Limited regions GM Approach Combine Conventional and Industrial Oils High oleic oils, Castor Goals •Higher functionality •High oleic •Low polyunsaturates •Multiple crops LINNAEUS Plant Sciences Four novel hydroxy fatty acids 0 2.5 5 7.5 10 12.5 15 17.5 min Ricinoleic acid (12OH-18:1 9) Densipolic acid (12OH-18:2 9, 15) Lesquerolic acid (14OH-20:1 11) Auricolic acid (14OH-20:2 11, 17) LINNAEUS Plant Sciences Comparison of Fatty Acid Profiles The HFA clearly replaces oxidatively unstable polyunsaturates Hyroxy Fatty Acid (HFA) Fatty Acid Name Hydroxy Ricinoleic Fatty Acid & Others Linnaeus Canola Castor Oil* Oil Oil % Fatty Acid 15.6 0.0 89.2 C16:1 Palmitoleic 0.3 C18:1 Oleic 59.1 60.9 3.5 C18:2 Linoleic 11.3 21.0 4.2 C18:3 Linolenic 3.4 8.8 C20:1 Gadoleic 1.0 C22:1 Erucic 0.7 C12:0 Lauric 0.0 C16:0 Palmitic 5.1 4.1 C18:0 Stearic 1.7 1.0 1.8 LINNAEUS *Expressed in Brassica napus (hydroxy fatty acids include ricinoleic and densipolic acid) Plant Sciences Soy Lines with HFA 50.0 '16:0 Percentage 40.0 '18:0 30.0 '18:1 20.0 '18:2 '18:3 10.0 ricinoleic 0.0 1 2 3 4 5 6 7 Line Number LINNAEUS Plant Sciences Industrial Applications For Linnaeus HFA Technology HFA Vegetable Oils Base Oil Feedstock Reactive Component Lubricants* Fuels Paints, Coatings and Inks Functional Fluids* Oleochemicals Polymers and Foams Process Oils * Discussed in current presentation LINNAEUS Plant Sciences Linnaeus HFA Oil As a Lubricant - Target Markets and Drivers Engine oils • Fuel economy, low phosphorus (ILSAC GF-4) & sulfur, reduced emissions, long drain Hydraulic oils • Improved lubricity and environmentally sensitive applications Two stroke oils • Aquatic toxicity, reduced emissions & smoke Chain bar lubricants • Environmentally sensitive applications LINNAEUS Plant Sciences Total Fina Elf Environmentally Friendly Products •Total Fina Elf products overlap application areas for the HFA technology •The Linnaeus oil is likely to offer specific benefits over current basestocks used by Total Fina Elf e.g. better lubricity from HFA, lower cost vs. synthetic esters Application Base Oil Motor Oil 4 Stroke Engine Oil Hydraulic Fluid 2 Stroke Oil Fuel Chain Bar Oil Feedstock Biodiesel Reactive Diluent TotalFinaElf EF Products Comments HTX766 HTX822 Hydrelf Bio 46, Total Biohydran (SE, TMP), Fina Biohydran RS Total Neptuna 2 Aquazole Total ChainBio Synthetic ester based oil Synthetic ester based oil Colza Rapeseed methyl ester Polymer Raw Material Rislan B Synthetic ester based oils, Vegetable oil based Synthetic oil based Emulsified diesel fuel Natural biodegradable Reaction of hydroxy group LINNAEUS Plant Sciences Why Combine Conventional & Specialty Oils? - Best of Both Worlds Castor Oil* •RBOT** Oxidation = 29 mins •Pour Point ~ -33C •Viscosity @ 40C = 255 cSt •Timken OK Load = 30 kg •4-Ball: Scar = 0.6, Coeff. Friction = 0.04 •High reactivity Linnaeus Oil*** •RBOT Oxidation = 18-25 mins •Pour Point ~ -15C to -21C •Range of viscosities ~50-120 cSt •Timken OK Load =20-30 kg •4-Ball: Scar ~ 0.6, Coeff. Friction ~ 0.06 •Moderate reactivity High Oleic Canola Oil* •RBOT Oxidation = 15 mins •Pour Point ~ -15C •Viscosity @ 40C ~ 37 cSt •Timken OK Load = 20 kg •4-Ball: Scar = 0.7, Coeff. Friction = 0.08 •Very low reactivity **RBOT = Rotary Bomb Oxidation Test (ASTM D2272) *** Based on blended oil data (10-60% HFA content) * Source: Vegetable Oils – Structure and Performance, S. Lawate, et al, 1997, Tribology Data Handbook, CRC Press, Ed. Rich Booser LINNAEUS Plant Sciences Linnaeus Oil Can Substitute Synthetic Esters - Comparison Property Test Method Kinematic Viscosity ASTM D2270 @ 40 ºC @ 100 ºC VI Pour Point (ºC) ASTM D97 Flash Point (ºC) ASTM D92 Toxicity Cost ($/lb) Need for Viscosity Modifier* * Based on blended oil data ** To obtain ISO 46 viscosity grade TMP Trioleate High Oleic Canola Oil 10% HFA Linnaeus Oil* 50 cSt 10 cSt 201 -33 >300 Possible neurotoxicity ~ $1-$1.30 37 cSt 8 cSt 212 -15 >300 50 cSt 10 cSt 180 -18 >300 None ~$0.65 None ~ $0.75 No Yes No LINNAEUS Plant Sciences Linnaeus HFA Oil As a Lubricant - Benefits • Will enable satisfaction of market drivers (previous slide) – Better lubricity, lower emissions, improved fuel economy, lower ecotoxicity, • Lower cost vs. synthetic esters • Oils with varying viscosity can be made by altering HFA content - currently this is a big shortcoming for vegetable oils - beneficial for formulation Blue Angel hydraulic fluids (can allow elimination of viscosity modifier) Based on blended oil data ISO Grade ISO 46 ISO 68 ISO 100 ISO 220 Required % Hydroxy Content ~10% ~20% ~60% 90% LINNAEUS Plant Sciences Motor Oil Opportunity LINNAEUS Plant Sciences What is the potential U.S. motor oil market? Replacing 10% of 1.1 billion gallons Automotive Other Bio potential 110 million gallons @ 50 gallons/acre would require 2.2 million acres! Value Proposition • Canola oil $.40 CDN per pound • Motor oil $1.25 USD per pound based on $2.50 quart • Add pour point and anti-oxidant package • What about emission credits? LINNAEUS Plant Sciences Toxic Waste Metals from Used Engine Oil TCLP Analysis: EPA 7470A and 6010B Methods Element Reporting Limit (ppm) Canola M.O. Mercury 0.0002 ND1 Arsenic 0.40 ND Barium 0.40 ND Cadmium 0.04 ND Chromium 0.20 ND Lead 0.20 ND Selenium 0.80 ND Silver 0.10 ND Sample oil derived from 2000 Ford Ranger Truck @ 3,300 miles (5,310 km) August 10, 2001. The oils remain nontoxic after normal engine use and can be disposed of or recycled safely. LINNAEUS 1ND = None detected (Aspen Analytical Lab) Plant Sciences USPS Ford Explorer Observations • Significant reductions in CO,CO2, HC and NOx • 1.3% Improvement in fuel economy • Approximately 300lb reduction in total tail pipe emissions per vehicle per year. • It doesn't work without HFA LINNAEUS Plant Sciences USPS Ford Explorer Emissions EPA Analysis Dr. Duane Johnson Supt., Northwest Ag Research Center Director Institute for Bio-Based Products MSU **** 8 7 6 ***1 5W30 * Canola M.O. 5 PPM 4 ** 3 2 **** 1 0 NonMethane HC CO CO2 / 10 HC X100 NOx X10 % Gas Reduction NMHC: 25% CO: 48% CO2: 1% HC: 32% NOx: 80% Emissions data from paired oil evaluations show significant decreases in all tailpipe emissions. 1 Probability: *= 0.05; **=0.01; ***=0.001; ****=0.0001 LINNAEUS Plant Sciences Fuel consumption with Titan GT 1 acc. to EG3 Test Data of test candidate PORSCHE Model 911 Carrera Coupé Speed 280 km/h Fuel Gasoline Cubic capacity 3,387 Liter Power 221 kW (300 PS) Max. revs 6800 1/min In comparison with SAE 15W-40: Fuel/Diesel-Consumption -6,4 % Emissions NOx -24 % MERCEDES-BENZ E 220 CDI 198 km/h Diesel 2,151 Liter 92 kW (125 PS) 4200 1/min -3,2 % -16 %. LINNAEUS Plant Sciences Why the Lowered Emissions? • Petroleum oil molecules range from 20-40 carbons in length and remain somewhat volatile when in contact with hot engine parts. In fact, thirty percent of HC and twenty percent of CO exhausted from an engine are from the motor oils. Vegetable motor oils are 55 to 65 carbons long and are virtually nonvolatile (no VOCs). • Without oil as a contributor of VOCs, engines can combust fuel at lower operating temperatures. This increases engine oxygen uptake providing a cleaner burn and engines run leaner (less fuel). LINNAEUS Plant Sciences One Example –US Navy • Total Oil Spilled (U.S. Navy statistics): 181,453 gallons 1,402 spills reported • Monthly use 600,000 gallons • Replace 10% or 720,000 gallons/yr • Requires 14,000 acres • Generate $7MMUS annually LINNAEUS Plant Sciences Additional Lubricant Markets • • • • • Hydraulic oils Metalworking Lubricants *Diesel additive Two-stroke oils Chain bar lubricants 210 68 66 19 3 2001 US Market in Million gallons National Petroleum Refiners Association *Canadian market estimates based on 2005 mandate LINNAEUS Plant Sciences Production Strategy • Develop transgenic lines - several labs • Use Bunge/Canamera HEAR Model – Bulk up seed – Contract grow/IP protect – Contract custom crush • Cargill, DOW and others follow similar model • Provinces interested in supporting IP LINNAEUS Plant Sciences Marketing Strategy • Use Kyoto/Monitization issues • Leverage Government policy: Some Provinces have ongoing initiatives. Fed’s evolving • Target Military as well as fleets • Possible partnership with TOTAL or others • Control sales to maximize value capture LINNAEUS Plant Sciences Marketing Strategy continued… • Bio-lubes have historically suffered from poor oxidative stability, high pour points. • Bio-technology is the solution – this is NEW • We are NOT traditional providers of veg. oil • Marketing/positioning requires environmental and life cycle consideration – this is how margin will be created. • Get high on the chain - OEM or better LINNAEUS Plant Sciences Regulatory Strategy Current CFIA Environment • Regulations/Guidelines in major transition • Introduction of Mol. Farming 12-18 months • New “guidelines” promise to be challenging • Opportunity exists to influence direction NOW LINNAEUS Plant Sciences Regulatory Strategy continued… • • • • • • Highlight environmental benefits Participate in all policy forums Ensure that we go in under PNT guidelines Use HEAR as model Stress edible aspect (GRAS) of HFA’s **Go for food and feed status LINNAEUS Plant Sciences Forecast According to The Chemical Market Reporter: “Annual growth rate for bio-based lubricants is projected at 7-10% compared to 2% for overall lubricants” LINNAEUS Plant Sciences Future of Oil Seeds • Next “canola” requires biotechnology • Canadians are world class producers • Infrastructure is in place for “big” products • Huge win - solve the expression problem • One possible platform technology: Look to Castor’s mechanism for bioreactors… LINNAEUS Plant Sciences Genomics/Proteomics of Castor How does Castor do it? • • • • • • • Long standing collaboration Prof. A. Slabas World leader in lipid metabolism 2.5MM committed over next 3 years List of known enzymes targeted Photoaffinity etc. for new discoveries Leverage 5 years of experience with Castor Support of TOTAL LINNAEUS Plant Sciences Summary • There have been dramatic improvements in biotech methods and tools • Systems exist to efficiently clone genes of interest • Patents allow control of novel materials • Regulatory approvals increasing • It is possible to own a feed stock • Plants hold the answer to CO2 issues LINNAEUS Plant Sciences