SBI4U
Name:
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Glycolysis Modelling Activity
Glycolysis is the first stage of cellular respiration. It has ten reactions that start with the movement of
glucose from the blood stream and into the cell. Glycolysis occurs in the cytoplasm, and is common to
eukaryotes and prokaryotes since this is an anaerobic process and does not require oxygen.
For this activity, you will be looking at a 3D model of all the chemicals involved with glycolysis. Use page
98 in your textbook to see what the molecules look like. At each change, highlight or circle the changes.
Add arrows and any coenzymes and answer the questions. Use green balls for phosphate groups. Identify
when there are two of each chemical made as well. Submit for evaluation.
1. Make a 3D model of glucose. Draw the 3D glucose in the box:
a. Where does the glucose come from?
2. Now build glucose-6-phosphate. Circle or highlight on the drawing
the changes.
a. Where did the phosphate group come from?
Glucose
G6P
b. What does the “6” represent?
3. Build fructose-6-phosphate. Again, identify the changes.
a. Explain the changes that make this now fructose.
F6P
b. What was responsible for this change?
4. Build fructose-1,6,-bisphosphate
a. What does “bis” represent?
Fructose-1,6,bisphosphate
b. Where did the phosphate group come from?
5. Now F-1,6-BP splits into two different
molecules.
a. What major change has occurred?
b. Which molecule is needed for the
next step?
DHAP
G3P
SBI4U
Name:
6. Build 1,3-bisphosphoglycerate (BPG)
a. Why are the numbers 1 and 3 used in the name?
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BPG
b. Why are there two of these chemicals in the diagram?
7. Build 3-phosphoglycerate (3PG)
a. Where did the phosphate group go?
3PG
8. Build 2-phosphoglycerate (2PG)
a. What has changed from 3-phosphoglycerate?
2PG
9. Build phosphoenolpyruvate (PEP)
a. What chemical has been removed in order to create this
molecule?
b. According to the textbook (pg. 97), what is the difference
between an “acid” and “-ate”? e.g., pyruvate vs. pyruvic acid
PEP
10. Build pyruvate
a. How many carbons are found in each pyruvate?
Pyruvate
b. What has changed between PEP and pyruvate?
Calculations:
1.
2.
3.
4.
# of NADH produced: _____
5. # of ADP produced: _____
# of water molecules produced: _____
6. # ATP produced: _____
# of carbons found in the glucose: _____
7. Net total of ATP produced: _____
# of carbons found in each pyruvate
molecule: _____
8. Energy conversion efficiency of glycolysis calculations
Energy conversion efficiency = energy from the net total ATP/glucose energy (Assume 50 kJ/mol
of energy per ATP and that each molecule of glucose carries 2870 kJ of energy)
9. Compare glycerol with glyceraldehyde and dihydroxyacetone to each other. Circle the
changes.