Gregor Mendel's Research and Principles Video Transcript Presented by Discovery Education “Bio Bit” is displayed on screen. Male Speaker: In the early nineteenth century, biologists believed that blending was responsible for pink offspring when you crossed red and white snapdragons. We now understand that this is not the case for the traits that Mendel studied. But how did he figure that out? Female Speaker: The reproductive structures of the pea plant are contained in each flower on the plant. They are the stamens and the pistil. A flower self-pollinates when pollen from the stamen is transferred, by wind or insects, to the sticky surface on the pistil. A pollen tube develops and grows down the pistil to the egg cell in the ovary. The sperm nucleus in the pollen tube combines with, and fertilizes the egg cell. Mendel had to ensure that he could control the parentage of seeds. He wrapped plants that he wanted to self-fertilize, so that they wouldn’t be pollinated and fertilized by other plants. He clipped off the amphids of flowers on other plants so that he could pollinate the plants himself with the pollen from other, specific plants. This is cross-pollination. It results in the fertilization of the egg cell of one plant by the sperm nucleus of another “Bio Discovery” is displayed on screen. Male Speaker: I must make this clear for the society of natural history. These plants all had terminal flowers: flowers that grow from the ends of the stem. After they have been self-fertilized, they produce plants that had terminal flowers. I called these plants my P-one generation, parent plants that I knew were true breeding. These plants produce flowers that grow from the side of the stem, axial flowers. After these plants had been self-fertilized, they all produced plants that had axial flowers. These plants are true breeding. I decided to make my first cross with true breeding plants, cross-fertilized true-breeding plants for terminal flowers with true-breeding plants for axial flowers. All the flowers produced in the F-one, or first felial generation, had axial flowers. I wondered where the terminal factor went. i decided to cross the F-one plants. the F-one plants were not truebreeding, that is they did not produce offspring like themselves. Of the plants of the F-two, or second felial generation, three quarters produced axial flowers and one quarter produced terminal flowers. The factor for terminal flowers had come back. Curious to know more, I let each plant of the F-two generation self-fertilize. Of the plants produced, one quarter were true-breeding for axial flowers, one half were not true-breeding, or hybrid, but produced axial flowers, and one quarter were true-breeding for terminal flowers. Clearly, the hybrid plants contained factors for both characteristics but produced only axial flowers. The factor for terminal flowers had not disappeared, it was only masked. When it combined with a plant that had a similar factor, it produced offspring that had terminal flowers. To explain these results, I believed that the hereditary factors must occur in pairs, these factors must separate in the parent plant, and then each parent must give one factor, randomly, to the next generation. I will call these factors either dominant or recessive. The dominant factor masks the recessive factor. In this case, the true-breeding axial plants have two dominant factors: capital A, capital A. The true-breeding terminal plants have two recessive factors, small a, small A, so the trait can show. The hybrid plant has one dominant factor, capital A, and one recessive factor, small A, but only the dominant factor, axial flower, shows. Produced by: Discovery Education www.discoveryeducation.com Transcribed August 2011 ©2011 Connections Education LLC. All rights reserved.