Photosynthesis lab
Outline of the day
1. Turn in your lab reports at the front
–
More than 10 minutes late = bad
Any questions on last week’s lab?
Quiz
Introduction to the lab
Lab!
Check out
2.
3.
4.
5.
6.
•
•
Get a stamp
Make sure I mark you down for attendance
Quiz
• Ends 10 minutes after it’s started
– Ends at: ____
Lab this week!
• Exploring photosynthesis!
– What pigments are used
– How light interacts with chlorophyll
– Effect of photosynthesis on carbon-dioxide
concentration
Plants do photosynthesis in leaves
and/or stems
CC licensed images from: http://flickr.com/photos/zachdurland/369427527/; http://flickr.com/photos/janinehealy/133056572/; http://flickr.com/photos/jayw/116902430/
Plants and algae have chloroplasts
• Contain highly folded membranes
– Called thylakoids
– Increases surface area exposed to light
• Chlorophyll is in these membranes
– Chlorophyll “harvests” light
Liverwort leaf cells
A chloroplast
Chloroplast PD by It'sJustMe at http://en.wikipedia.org/wiki/Image:Chloroplast-new.jpg
Chloroplast in liverwort cells from Wellcome images http://images.wellcome.ac.uk/ B0005648 Credit M I Walker, Wellcome Images
Summary of “typical”
photosynthesis
Light
“Light reactions”
Chlorophyll-mediated
O2
H2O
ATP
CO2
NADPH
“Dark reactions”
Calvin cycle
Sugars
Note: In plants, all these reactions occur in the chloroplast
Lab hints:
• Put on lots of the photosynthetic concentrate for
the pigment part
• Make sure your extract is crystal-clear after it’s
been filtered
• Stop blowing into the phenol red as soon as it
turns yellow
– If you go too long, it will take hours to turn back!
Before you leave
• Clean up your work area
– Wash glassware and store upside down
• Show me your lab report so I can stamp it
– Need to have all data fields filled in
– Complete at home and then turn in at the beginning of
next lab
• Remember that we’ll have a quiz at the
beginning of the next class
– 6-7 questions on today’s lab
– 3-4 questions on the lab we’ll do next week
Notes for the instructor:
• Add any relevant cleanup instructions to the final
slide (that slide is a generic one I’m adding to
each presentation).
• I haven’t been able to find a good, single-image
creative-commons licensed summary of
photosynthesis that shows the inputs and
outputs clearly (which I’ve indicated is missing
on the photosynthesis summary slide in the
presentation). I’ve sketched out the basics of
what I’m looking for, but if you find a good one
please let me know.
• I’d also love a better diagram/image of a single
chloroplast.
License information
• This work is licensed under the Creative Commons AttributionNonCommercial-ShareAlike 3.0 License. To view a copy of this
license, visit http://creativecommons.org/licenses/by-ncsa/3.0/us/ or send a letter to Creative Commons, 171 Second
Street, Suite 300, San Francisco, California, 94105, USA.
• The slides in this presentation were originally created by Marc
C. Perkins (http://faculty.orangecoastcollege.edu/mperkins).
• You are free to use, modify, and distribute these slides
according to the terms of the Creative Commons license (e.g.,
you must attribute the slides, no commercial uses are allowed,
and future distributions must be licensed under a similar
license).
• Attribution should be given to Marc C. Perkins (and any later
editors), including a link back to Marc’s current website. This
applies both while distributing the slides and during use of the
slides; attribution during use can be satisfied by, for instance,
placing small text on at least one of the slides that has been
shown (see below for an example).
History
• August 2007: Marc Perkins released first
version.
http://faculty.orangecoastcollege.edu/mperkins
(If you modify these slides and redistribute them, add your information to the list)
Extra slides
Summary of “typical”
photosynthesis
Missing image:
Summary diagram of
photosynthesis that shows
the inputs and outputs
clearly.
Summary of “typical”
photosynthesis
Light
“Light reactions”
Chlorophyll-mediated
O2
H2O
ATP
CO2
NADPH
“Dark reactions”
Calvin cycle
Sugars