Chapter 14: DNA and RNA revision worksheet 1. Define the following: (i) Heredity: _____________________________________________________________________ _____________________________________________________________________ (ii) Gene: _____________________________________________________________________ _____________________________________________________________________ (iii) Gene expression: _____________________________________________________________________ _____________________________________________________________________ 2. What two substances make up a chromosome? ___________________________________________________________________________ 3. Distinguish between coding and non-coding regions of a chromosome: ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ 4. What does DNA stand for? ____________________________________ 5. Name the three components of a nucleotide. (i) ________________________ (ii) ________________________ (iii) ________________________ 6. Which part of the nucleotide varies? _____________________ 7. Name the four possibilities of this part of the nucleotide in DNA. (i) ______________________ (ii) ______________________ (iii) ______________________ (iv) ______________________ 8. In the space below, draw the structure of DNA showing 3 pairs of nucleotides in DNA with the following sequence on one strand: ATC 9. Explain what you understand by ‘complementary base-pairing’: ___________________________________________________________________________ ___________________________________________________________________________ 10. Briefly describe the process involved in DNA replication: ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ 11. What is DNA profiling? ___________________________________________________________________________ ___________________________________________________________________________ 12. Briefly describe the four distinct stages in DNA profiling: (i) _____________________________________________________________________ _____________________________________________________________________ (ii) _____________________________________________________________________ _____________________________________________________________________ (iii) _____________________________________________________________________ _____________________________________________________________________ (iv) _____________________________________________________________________ _____________________________________________________________________ 13. What is genetic screening? ___________________________________________________________________________ ___________________________________________________________________________ 14. Explain the ethical problems associated with genetic screening: ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ 15. Answer the following questions with regard to the experiment you carried out to isolate DNA from plant tissue: (i) Name the plant that you used: _______________________ (ii) Explain the importance of salt in this experiment: _____________________________________________________________________ (iii) Explain the importance of washing up liquid in the experiment: _____________________________________________________________________ _____________________________________________________________________ (iv) Explain the importance of the heating step: _____________________________________________________________________ _____________________________________________________________________ (v) How did you separate the DNA mixture from the other remnants of the plant cells (e.g. plant cell wall): _____________________________________________________________________ _____________________________________________________________________ (vi) How did you remove the protein from the DNA? _____________________________________________________________________ _____________________________________________________________________ (vii) Why was ice-cold ethanol used rather than ethanol at room temperature? _____________________________________________________________________ _____________________________________________________________________ 16. What does RNA stand for? ________________________________________ 17. Give two structural differences between DNA and RNA: ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________ ___________________________________________________________________________