Topic 2.2 Prokaryotic Cells IB Biology 2010 Objectives 2.2.1-Draw and Label a diagram of the ultrastructure of Escherichia coli as an example of a prokaryote. 2.2.2 -Annotate the diagram with the functions of each named structure. 2.2.3-Identify structures from 2.2.1 in electron micrographs of E. coli 2.2.4-State that prokaryotic cells by binary fissions What is a Prokaryotic Cell? Cells are often divided into certain groups based on major characteristics ◦ One such groupings divide cells into two groups: prokaryotic and eukaryotic Prokaryotic cells are much smaller and simpler than eukaryotic cells ◦ Most prokaryotic cells are less than 1 micrometer in diameter Feature of prokaryotic cells Study Figure and be sure you can identify: ◦ ◦ ◦ ◦ ◦ Cell wall Plasma membrane Flagella Ribosomes Nucleoid Electron micrographs of E. coli Cell Wall Prokaryotic cell wall protects and the maintains the shape of the cell ◦ Composed of carbohydrate-protein complex called Peptioglycan ◦ Some bacteria have an additional layer of a type of polysaccharide outside the cell wall This layer makes it possible for some bacteria to adhere to structures such as teeth, skin, and food Plasma membrane Located just inside the cell wall and has a composition similar to the membrane of eukaryotic cells ◦ Functions-controls the movement of materials in and out of the cell. It also plays a role in binary fission Cytoplasm—occupies the complete interior of the cell Pili and Flagella Pili-hair-like growths on the outside of the cell wall ◦ Used for attachment, and in joining bacterial cells in preparation for the transfer of DNA from one cell to another Flagella-they are longer than the pili and allow cell motility Ribosome Occur in all prokaryotic cells and they function as sites of protein synthesis ◦ They occur in very large numbers in cells with high protein production ◦ Cause a granular appearance to an electron micrograph The nucleoid region It is non-compartmentalized Contains a single, long, continuous, circular thread of DNA ◦ Region is involved with cell control and reproduction Plasmids-small, circular DNA molecules that are not connected to the main bacterial chromosome ◦ Replicate independently of the chromosomal DNA ◦ Not needed for everyday functions but does help adapt to unusual circumstances Binary Fission Prokaryotic cells divide by a very simple process called binary fission ◦ During this process, the DNA is copied, the chromosmes become attached to different regions on the plasma membrane, and the cell divides into two genetically identical daughter cells. ◦ This divisional process includes an elongation of the cell and a partitoning of the newly produced DNA by microtubule-like fibers made of protein called FtsZ. Binary Fission Summary Here is a list of major distinguishing characteristics of prokaryotic cells ◦ Their DNA is not enclosed within a membrane and is one circular chromosome ◦ Their DNA is free; it is not attached to proteins ◦ They lack membrane-bound organelles. Ribosomes are complex structures within the plasma membrane, but they have not exterior membrane ◦ Their cell wall is made up of Peptidoglycan ◦ They usually divide by binary fission ◦ They are characteristically small in size, usually 1-10 µm