BEWARE: This game alone will not prepare you for all topics on the exam. Also, use the midterm sem1 2015 jeopardy parts 2 and 3. Note that the jeopardy games do not cover topics of enzymes or DNA expression. These are covered in a second jeopardy game. Midterm Exam Review, 2015: Jeopardy, Double & Final Jeopardy Kingdoms & Life Cells Cell Energy Transport DNA structure $100 $100 $100 $100 $100 $200 $200 $200 $200 $200 $300 $300 $300 $300 $300 $400 $400 $400 $400 $400 $500 $500 $500 $500 $500 FINAL ROUND SWK/Life: $100 Question The statement “If the students are given candy then they will do well on the final exam” is an example of this. ANSWER BACK TO GAME SWK/Life: $100 Answer If/then prediction (hypothesis would state: Students knowing candy will be given as a reward for a passing test score will earn passing scores more often than students NOT being told about a reward.) BACK TO GAME SWK/Life: $200 Question These are the 7 characteristics of life. ANSWER BACK TO GAME SWK/Life: $200 Answer Reproduction Heredity/presence of DNA Evolution/adaptation Growth and Development Metabolism/chemical reactions & energy conversions Homeostasis Cellular Organization BACK TO GAME SWK/Life: $300 Question This characteristic describes when a population of a species of organisms changes over time due to highest rates of reproduction of best adapted individuals. ANSWER BACK TO GAME Passive : $300 Answer Evolution BACK TO GAME SWK/Life: $400 Question Which two Kingdoms contain prokaryotic cells? ANSWER BACK TO GAME SWK/Life: $400 Answer Eubacteria and Archaebacteria BACK TO GAME SWK/Life: $500 Question List the four Kingdoms that have eukaryotic cells. ANSWER BACK TO GAME SWk/Life: $500 Answer Fungi, Plant, Animal, Protist BACK TO GAME Cells: $100 Question This is the part of a eukaryotic cell where DNA is stored. ANSWER BACK TO GAME Cells: $100 Answer The Nucleus BACK TO GAME Cells: $200 Question Which organelle is responsible for protein synthesis in both prokaryotes and eukaryotes? ANSWER BACK TO GAME Cells: $200 Answer Ribosomes ( on the rough ER and loose in the cytoplasm) BACK TO GAME Cells: $300 Question Which organelle contains digestive enzymes and cleans up the cell by “eating” old dead cell parts so that they can be recycled? ANSWER BACK TO GAME Cells: $300 Answer Lysosomes BACK TO GAME Cells: $400 Question Which eukaryotic cell organelle modifies proteins or lipids, then packages them into transport vesicles that are often transported to the cell membrane for export? ANSWER BACK TO GAME Cells: $400 Answer Golgi Apparatus BACK TO GAME Cells: $500 Question What theory attempts to explain how eukaryotic cells developed? Describe the fundamentals of the theory. ANSWER BACK TO GAME DNA: $500 Answer Endosymbiosis - prokaryotic cells combined (through endocytosis or through symbiosis) and ended up forming the membrane bound organelles (especially mitochondria and chloroplasts) in eukaryotic cells. BACK TO GAME Cell Energy: $100 Question This is the source of all energy in our food chain. ANSWER BACK TO GAME Cell Energy: $100 Answer Sunlight BACK TO GAME Cell Energy: $200 Question This is the most important reason that all Organisms must carry out cell respiration, Even autotrophs. ANSWER BACK TO GAME Cell Energy: $200 Answer Making or charging ATP BACK TO GAME Cell Energy: $300 Question These are the balanced chemical equations for photosynthesis and cellular respiration. ANSWER BACK TO GAME Cell Energy: $300 Answer Cellular Respiration: C6H12O6 + 6O2 -> 6CO2 + 6H2O + ATP Photosynthesis: 6CO2 + 6H2O + Sunlight -> C6H12O6 + 6O2 BACK TO GAME Cell Energy: $400 Question These are the reactants and products of the light-independent reactions (also called dark reactions or the Calvin Cycle) of photosynthesis. ANSWER BACK TO GAME Cell Energy: $400 Answer Reactants: NADPH, CO2 and ATP NADPH carries electrons & protons that combine with carbon dioxide to produce sugars; producing sugars is endothermic, so ATP provides the energy that must be absorbed. Products: carbohydrates, NADP+, ADP, Phosphate NADP+ will be recharged during the next light reaction cycle when it accepts electrons & protons released when water is split; energy of sunlight is used in the endotermic regeneration of ATP from BACK TO GAME ADP & phosphate Cell Energy 500 This process allows the human muscle cells to produce ATP when there is not enough oxygen for aerobic respiration. ANSWER BACK TO GAME Cell Energy 500 Lactic Acid Fermentation this process regenerates NAD+ necessary to allow repeated cycles of anaerobic glycolysis— fermentation DOES NOT allow the cell to extract any more energy than glycolysis alone BACK TO GAME Transport: $100 Question These molecules are the main building blocks of the cell membrane. They are both polar and non-polar. ANSWER BACK TO GAME Transport: $100 Answer Phosphate Head – Polar, Hydrophilic Phospholipids Lipid Tail – Nonpolar, Hydrophobic BACK TO GAME Transport: $200 Question This is the passive transport of water from an area of higher to lower water concentration across a semi-permeable membrane. Another way to think of this is movement of water from a region where solute concentration is higher to where it is lower. ANSWER BACK TO GAME Transport: $200 Answer Osmosis BACK TO GAME Transport: $300 Question This is the hydrophobic part of the cell membrane. Explain… ANSWER BACK TO GAME Transport: $300 Answer The lipid tails of the phospholipids in the center of the phospholipid bilayer are non-polar, making them hydrophobic. BACK TO GAME Transport: $400 Question Refer to the dual nature of the phospholipid bilayer to explain why water doesn’t freely flow across the membrane, but rather requires passage through aquaporin channels. ANSWER BACK TO GAME Transport: $400 Answer Water is a polar molecule (unequal distribution of electrons). This means water molecules are unable to pass through the non-polar fatty acid lipid tails in the cell membrane. Aquaporins (protein channels) are integrated into the cell membrane and allow water to pass without requiring contact with the hydrophobic membrane core.. BACK TO GAME Transport: $500 Question A cell has 10% solute in its cytoplasm. If it is bathed in an environment solution having 5% solute, is the environment: a hypotonic environment b isotonic environment c hypertonic environment ANSWER BACK TO GAME Transport: $500 Answer A) Hypotonic – there is more water (95%) outside than inside the cell (90%). Therefore water will flow into the cell. remember hypo- means “too little”& –tonic means “solute to be at Solute equilibrium” 5% 95% Water 10% Solute 90% Water BACK TO GAME DNA: $100 Question What is the name of the monomer that makes up DNA, as well as the type of polymers than include DNA? ANSWER BACK TO GAME DNA: $100 Answer Monomer: deoxyribonucleotide Polymer: Nucleic Acid BACK TO GAME DNA / Protein Synth: $200 Question What are the three parts of every deoxyribonucleotide in deoxyribonucleic acid? ANSWER BACK TO GAME DNA: $200 Answer Deoxyribose sugar, phoshate group, and nitrogenous base BACK TO GAME DNA: $300 Question What are the names of the 4 possible nitrogenous bases in deoxyribonucleotides? ANSWER BACK TO GAME DNA: $300 Answer Adenine (A), Thymine (T), Guanine (G), cytosine (C) BACK TO GAME DNA: $400 Question What parts of DNA make up the covalently bonded backbone of each side of the double helix? What parts center rungs where the two halves of the helix are joined by weaker hydrogen bonds? ANSWER BACK TO GAME DNA: $400 Answer alternating deoxyribose sugars and phosphate groups make up the backbone, while complementary base paired nitrogen bases form the rungs BACK TO GAME DNA: $500 Question The double stranded DNA molecule is “anti-parallel” in orientation and held together by “complementary base pairing”. What does this mean? ANSWER BACK TO GAME DNA: $500 Answer Anti-parallel means that One strand is oriented with the phosphate on the same end where the other strand ends in a deoxyribose sugar (they face opposite directions; complementary base pairing means that A pairs with T, while C pairs with G. BACK TO GAME FINAL ROUND Question Draw and label a diagram that shows how photosynthesis and cell respiration are interdependent within the biosphere. ANSWER BACK TO GAME FINAL ROUND, Jeopardy, Question BACK TO GAME Double Jeopardy Midterm December, 2015, Review Kingdoms & Life Cells Cell Energy Transport DNA structure $200 $200 $200 $200 $200 $400 $400 $400 $400 $400 $600 $600 $600 $600 $600 $800 $800 $800 $800 $800 $1000 $1000 $1000 $1000 $1000 FINAL ROUND SWK/Life: $200 Question What is the difference between everyday use of the word theory versus the scientific use of the word theory.? ANSWER BACK TO GAME SWK/Life: $200 Answer In everyday language, “theory” refers to an idea that has not been tested and is not supported by evidence; in science, “theory” refers to an idea that has been tested many times by many scientists and is supported by abundant, replicable evidence. BACK TO GAME SWK/Life: $400 Question Which characteristic of life is the requirement of every organism for chemical reactions and energy conversions? ANSWER BACK TO GAME SWK/Life: $400 Answer Metabolism—the term metabolism also means all of the chemical reactions of a cell, some of which are energy releasing (exothermic) and some of which are energy absorbing (endothermic) BACK TO GAME SWK/Life: $600 Question Which characteristic of life refers to the genetically-controlled predictable changes that occur between birth and death? ANSWER BACK TO GAME Passive : $600 Answer Growth and development BACK TO GAME SWK/Life: $800 Question Which two kingdoms contain cells having neither a nucleus or membrane bound organelles? ANSWER BACK TO GAME SWK/Life: $800 Answer Eubacteria and Archaebacteria BACK TO GAME SWK/Life: $1000 Question Which kingdom contains eukaryotic heterotrophs whose cells contain neither chloroplasts, large central vacuoles, nor cell walls? ANSWER BACK TO GAME SWk/Life: $1000 Answer Animalia (animal) BACK TO GAME Cells: $200 Question This is the part of autotrophic eukaryotic cells where photosynthesis occurs. ANSWER BACK TO GAME Cells: $200 Answer chlorophlasts BACK TO GAME Cells: $400 Question What part of a cell provides a framework for it’s shape, allows it to move, and provides a system of highways along which transport vesicles and organelles are moved through the cytoplasm.? ANSWER BACK TO GAME Cells: $400 Answer cytoskeletal proteins in the cytoplasm (and for cell movement, also those in the flagella and cilia of some cells) BACK TO GAME Cells: $600 Question Which organelle is located inside the nucleus and is the site of assembly of ribosome subunits that are later shipped to the cytoplasm? ANSWER BACK TO GAME Cells: $600 Answer nucleolus BACK TO GAME Cells: $800 Question Name three organelles that are directly involved of synthesis, then modification and transport, of proteins—like hormones—that are exported out of the cell. ANSWER BACK TO GAME Cells: $800 Answer 1st the ribosome translates the messenger RNA, 2nd the rough endoplasmic reticulum packages some proteins into transport vesicles that will either be shipped to other organelles or to the golgi, 3rd some of the proteins in the RER produced transport vesicles are further processed in the golgi so that they will exit the cell via exocytosis BACK TO GAME Cells: $1000 Question Which kingdom includes prokaryotic extremophiles, such as halophiles that live in very salty water, thermophiles that live in hot water, and acidophiles that live in acid bogs?. ANSWER BACK TO GAME DNA: $1000 Answer Archaebacteria. BACK TO GAME Cell Energy: $200 Question Prokaryotes do not contain any membrane bound organelles, not ever chloroplasts and mitochondria. Do heterotrophic bacteria carry out cell respiration? Do autotrophic bacteria carry out cell respiration, photosynthesis, neither process or both processes? ANSWER BACK TO GAME Cell Energy: $200 Answer All cells require ATP, so all cells carry out cell respiration; in prokaryotes, the process is a little different than in eukaryotes. All photosynthetic autotrophs require the chemical reactions of photosynthesis, but the process is a little different in prokaryotes; all autotrophic bacteria also require cell respiration, but again, the process is a little different versus eukaryotes. BACK TO GAME Cell Energy: $400 Question Describe the ATP cycle, showing how cells use it both to store energy in ATP and to release energy from ATP to do cell work. ANSWER BACK TO GAME Cell Energy: $400 Answer ATP energy for cell work & some heat + ADP + phosphate ADP + phosphate + energy released from organic compounds during cell respiration ATP BACK TO GAME Cell Energy: $600 Question Combustion is described by the same chemical reaction as aerobic cellular respiration. Why would cellular combustion kill cells, whereas aerobic cell respiration is required for cell survival? ANSWER BACK TO GAME Cell Energy: $600 Answer Aerobic Cellular Respiration—many different chemical reactions serves as steps gradually releasing the chemical potential energy of organic compounds C6H12O6 + 6O2 -> 6CO2 + 6H2O + 34ATP + some heat released gradually over several different steps NOT enough heat is released to denature enzymes Combustion—a single chemical reactions C6H12O6 + 6O2 -> 6CO2 + 6H2O + heat only, all of which is released simultaneously The temperature rises to above the point at which enzymes would denature, killing cells. BACK TO GAME Cell Energy: $800 Question Which phase of the process of photosynthesis splits water to release oxygen? _____ Which phase absorbs Carbon dioxide, then uses it to build carbohydrates? ______ ANSWER BACK TO GAME Cell Energy: $800 Answer Light dependent phase in thylakoids slits water, releases oxygen gas, loads energy rich electrons onto electron carrier NADP+, and traps energy of sunlight in ATP Light independent/dark reactions/calvin cycle in stroma use energy of ATP, electrons and protons of NADPH, and Carbon dioxide to build sugars BACK TO GAME Cell Energy $1000 What are the two types of fermentation, and what type of organisms carry out each type? ANSWER BACK TO GAME Cell Energy $1000 Lactic Acid Fermentation—bacteria and mammal muscle; alcoholic fermentation—yeasts which are unicelluar fungi BACK TO GAME Transport: $200 Question Name the three types of passive transport across cell membranes, and explain how these are similar and different. ANSWER BACK TO GAME Transport: $200 Answer Simple diffusion Facilitated diffusion osmosis Requires no transport protein Requires solute-specific Requires aquaporin channel or pore protein channel proteins Moves small, uncharged solutes directly between phospholipid molecules in the bilayer Moves large or charged solutes through openings inside transport proteins— each channel only fits a particular solute Moves water only through the opening in aquaporin channel Moves solute down the concentration gradient, from higher to lower concentration cell approaches equilibrium (= solute concentration BACK TO GAME Transport: $400 Question Compare similarities and differences of active transport and facilitated diffusion. ANSWER BACK TO GAME Transport: $400 Answer Active transport of solute Facilitated diffusion of solute Requires a solute-specific membrane transport protein called a pump OR motor proteins for exocytosis and endocytosis Requires a solute-specific membrane transport protein called a channel or pore Cell expends its own energy to power the process, usually ATP Cell does not expend energy; conc gradient collapse fuels process Moves cell away from conc equilibr Moves cell towards conc equilibrium Solutes move from lower to higher concentrn, up/against conc gradient Solutes move from higher to lower conc’n, Down/With conc’n gradient Includes exocytosis that uses motor proteins/ATP to move vesicles filled w/ large amounts material (or large particles) for release out of cell OR endocytosis that pinches in vesicles Works only with solutes small enough to pass through a transport protein; is not a form of bulk transport BACK TO GAME Transport: $600 Question These are proteins that extend outwards from the cell membrane and into the surrounding environment; on their external surfaces, many of these proteins have attached carbohydrate chains. ANSWER BACK TO GAME Transport: $600 Answer Cell identification molecules BACK TO GAME Transport: $800 Question What is different about a peripheral membrane protein versus an integral membrane protein? ANSWER BACK TO GAME Transport: $800 Answer Peripheral membrane proteins are attached to either the cytoplasmic or the extracellular face of the plasma membrane, whereas integral membrane proteins extend all the way from one face to the other. BACK TO GAME Transport: $1000 Question Why is it necessary to define both the tonicity (relative solute concentrations) of the cell’s cytoplasm AND the solution in the cell’s environment unless the cell is at osmotic equilibrium? ANSWER BACK TO GAME Transport: $1000 Answer Unless cell is at osmotic equilibrium (isotonic, with equal internal & external solute concentrations), either the cell will be hypertonic & the environment hypotonic or visa versa (because osmosis is fueled by conversion of potential energy as solute moves down its concentration gradient) 5% Solute 95% Water hypotonic environment 10% Solute 90% Water Hypertonic cytoplasm BACK TO GAME DNA: $200 Question Long before the discovery of DNA’s structure, scientists had discovered that the nucleus contains the genetic material of a cell, as well as that nuclei contain both DNA and protein. What scientists discovered that the genetic material is DNA, not protein? ANSWER BACK TO GAME DNA: $200 Answer Avery and his collaborators were able to transform (change the traits) of a species of bacteria, changing it from a harmless strain to one that kills mice due to pneumonia, simply by adding the killer cell DNA to live harmless cells. Adding the proteins of the killer cells did not transform the cells. BACK TO GAME DNA / Protein Synth: $400 Question What scientist contributed the knowledge that showed %C =%G, while %A=%T? ANSWER BACK TO GAME DNA: $400 Answer Chargaff isolated DNA, then analyzed it biochemically. He found that the DNA contained nitrogenous bases A, T, C, and G, the four of which comprised 100% of the Nitrogenous bases in DNA. He also found that if C is present at 30%, then G is present at 30%, and T will be present at 20% and A at 20%. (other scientists who made major contributions to the discovery of DNA structure were: Franklin—Xray of DNA showed that it was a double helix; Watson and Crick—built model matching today’s current description of DNA structure by synthesizing data generated by Chargaff, Franklin, and others) BACK TO GAME DNA: $600 Question Compare and contrast the structures of DNA and RNA. ANSWER BACK TO GAME DNA: $600 Answer DNA-deoxyribonucleic acid RNA-ribonucleic acid Monomers—deoxyribonucleotides having the sugar deoxyribose, phosphate, and nitrogenous base Monomers—ribonucleotides having the sugar deoxyribose, phosphate, and nitrogenous base Nitrogenous bases: A T C G Nitrogenous bases: A U C G Double stranded Single stranded One type Several types, including mRNA, tRNA, rRNA Contains introns in eukaryotes Does not contain introns Made by replication Made by transcription BACK TO GAME DNA: $800 Question Name the complementary base pairs of: DNA bonded to DNA DNA bonded to RNA RNA bonded to RNA ANSWER BACK TO GAME DNA: $800 Answer DNA bonded to DNA A to T, C to G, T to A, G to C DNA bonded to RNA A to U, C to G, T to A, G to C RNA bonded to RNA U to A, C to G, A to U, G to C BACK TO GAME DNA: $1000 Question Describe 2 major classes of point mutations, and explain how each impacts the structure of DNA. ANSWER BACK TO GAME DNA: $1000 Answer Point mutations are single nucleotide alterations in DNA’s deoxyribonucleotide sequence. These may arise due to errors during DNA replication OR due to DNA damage/faulty repair after exposure to mutagens. Substitution point mutations result in replacement of a nucleotide with another (like changing an A to a C), whereas frameshift mutations result from insertion of extra nucleotides OR deletion of nucleotides. These insertions and deletions alter the combination of nucleotides into codons. BACK TO GAME FINAL ROUND Question Explain the difference between an “expressed mutation” and a silent mutation. ANSWER BACK TO GAME FINAL ROUND Answer Silent mutations Expressed mutations Either result from changes in sequence in DNA lying between genes OR lying within noncoding introns of genes OR Result from changes in DNA sequence that alter the amino acid coding of the gene. So, the protein coded has the wrong amino acid sequence and therefore also the wrong shape and altered function. Due to substitution mutations that switch one codon for another codon that codes for the same amino acid. Some expressed mutations affect noncoding parts of genes involved in regulating which cells express the gene, when cells make the protein, or how much protein they make. In either case, then change in DNA does not result in the amino acid sequence of a protein; so the protein coded by the gene experiences no change in shape or function. BACK TO GAME $2000 question Compare and contrast prokaryotic and eukaryotic cells. List at least 4 similarities and 4 differences. ANSWER $2000 Answer Prokaryotes Evolutionarily older—the ancestors of eukaryotes Eukaryotes Evolutionarily newer— descendents of prokaryotes •No nucleus •Nucleus •Smaller and simpler cells •No membrane bound organelles Example: Eubacteria and Archaebacteria •Larger and more complex cells •Have membrane bound organelles Examples: •Plants •Animals •Fungi •Protists Both Grow & develop •Living things Made of cells •DNA is genetic or hereditary •Ribosomes •Cytoplasm •Cell membrane carry out metabolism Reproduce by division Respond to stimuli Maintain homeostasis Adapt, evolve BACK TO GAME