Microbial Polysaccharides 1. Cell Walls of Gram-Positive and GramNegative Bacteria 2. Peptidoglycan 3. Lysozyme 4. Penicillin 5. Lipopolysaccharides of Gram-Negative Bacteria Classification of Bacteria by GramStaining Christian Gram, Danish physician about 120 years ago. Gram-positive bacteria retain the stain (crystal violet) and appear blue, whereas gram-negative bacteria usually appear pink due to the counterstain safranin. Crystal Violet Streptococci & E. coli Peptidoglycan Structure (in Staphylococcus aureus only) (in Staphylococcus aureus only) Lysozyme - discovered by Fleming in 1922. - present in tears, saliva, nasal secretions, etc., and also present in egg white. - lyses the cell walls of many bacteria Alexander Fleming Penicillin - discovered by Fleming in 1928. - secreted by the mold Penicilium notatum. - lysed many different bacteria. In the naturally occuring penicillin G (benzyl penicillin), R is a benzyl group. In the semi-synthetic penicillin derivative ampicillin, R is an aminobenzyl group. Penicillin - discovered by Fleming in 1928. - secreted by the mold Penicilium notatum. - lysed many different bacteria. Penicillin functions by specifically interfering with the cross-linking of peptidoglycan chains, a process that is catalyzed by glycopeptide transpeptidase. In S. aureus, the transpeptidase reaction involves an attack of the amino group of the pentaglycine bridge on the peptide bond between two D-ala residues to form a cross-link. An acyl-enzyme intermediate is formed in the transpeptidation reaction. Penicillin mimics the D-ala-D-ala moiety of the normal substrate and also forms an acyl-enzyme complex. However, the penicilloyl-enzyme complex is very stable. The result is that one penicillin molecule permanently inactivates one enzyme molecule. Penicillin resistance: Many pathogenic bacteria that are resistant to penicillin produce a b-lactamase (penicillinase). Lipopolysaccharides (Endotoxins) Drugs that are injected and medical instruments must be “pyrogen-free.” “Sterile” is not good enough.