Saving Hermit! Muslim Interscholastic Tournament Science Fair 2014 INTRODUCTION The world’s amphibian population has been in rapid decline for the past 50 years. Their population is declining as fast as the dinosaurs’ did 65 million years ago. This is an issue because amphibians are indicator species: based on their health and their numbers, the healthiness of an area can be determined. There are many factors contributing to the decline, like competition and the destruction of habitat, but 3 possible factors were tested: competing European earthworm, Lumbricus terrestris, population, vary pH levels, and varying nitrogen levels. A study was done at Watkins Park to test these possibilities. This experiment only focuses on earthworms since data from varying pH and nitrogen levels were inconclusive. PROBLEM What is limiting the amphibian population at Watkins Park? HYPOTHESIS If both earthworms and amphibians are competing for the same resources, such as space and food, then, earthworms would limit the amphibian population at Watkins Park. VARIABLES • Independent variables: 1. Population of Earthworms • Dependent variables: 1. Population of Amphibians MATERIALS 1. 2. 3. 4. 20 1ft. x 1ft. x 1in. wooden boards 20 flags numbered from “1” to “20” Amphibians and earthworms in Watkins Park Binder with Data Sheets and Pen PROCEDURE 1. Prior to the start of the experiment, 20 one foot by one foot by one inch wooden board habitats were set up at Watkins Park 2. The wooden boards were placed randomly in parallel of the stream and the Upland Trail, the range was about 100 feet. With flags, the first wooden board, located on the southern tip of the creek, was labeled “1” and each boards was continued to labeled accordingly as they were randomly placed more north. 3. At the start if the experiment, the artificial habitats were visited on nine separate over the course of ten weeks. 4. While these boards were looked under, the number of amphibians and earthworms present were counted and were noted in the binder with the data sheets in them. 5. pH levels and nitrogen levels were also noted. RESULTS/ANALYSIS • In graph and table A, a bar graph was made and an unpaired t-test was conducted with a p-value that was potentially zero. So there was a significant difference in the number of amphibians and earthworms present at Watkins Park. • In graph and table B, a scatter plot was made and the correlation, r, was calculated and r was calculated to be -.274, so there is a slightly negative correlation in the population of amphibians versus the population of earthworms. CONCLUSION The hypothesis is supported by the data collected. Earthworms may serve as a possible factor in the limitation of amphibians at Watkins Park. This is known because there is a significant difference in the population of amphibians and earthworms at Watkins Park. In addition, according to the slightly negative correlation, there is a somewhat correlation in the population of amphibians versus the population of earthworms at Watkins Park. Also Lumbricus terrestris, is known as an invasive species in most of the world, not just at Watkins Park. So it is safe to assume that they are a contributing factor to the decline in amphibians. Varying pH and nitrogen levels tests were conducted but the the results were proven to be inconclusive. ACKNOWLEDGEMENTS I would like to give a special thank you to…. • Allah (subhanahu wa’ ta’ala), this project would not have been possible without him. • Our MIST coaches for assisting us while conducting this experiment. • Our parents for driving us around and buying us materials for this project. • Watkins Park for allowing us to use their facility for our experiment. • I.S. and S.B. for allowing us to use some of their research. THEME INTEGRATION Because amphibians are indicator species, they help us humans determine if an ecosystem is safe for fishing, hunting, agriculture and much more that is needed for the sustainability of humans. Without amphibians, there would be significantly more mosquitoes, which carry harmful diseases to humans. Amphibians exemplify generosity when occupying their niche in the ecosystem. BIBLIOGRAPHY/REFERENCES 1. 2. 3. 4. 5. 6. 7. 8. Blaustein, A., R., Bancroft, B., A. (2007) Amphibian Population Declines: Evolutionary Considerations. BioScience (2007) Vol. 57 No. 5 Bickelhaupt, D. (2014). Soil pH: What it Means. Soil pH: What it Means. Retrieved from http://www.esf.edu/pubprog/brochure/soilph/soilph.htm Brady, N. C., Weil, R. R. (1996). The nature and properties of soils (11th ed.). Pgs 332-337. Upper Saddle River, NJ: Prentice-Hall, Inc. Boyle, P., Grow, S. (2008) The Global Amphibian Crisis. Endangered Species Bulletin (2008) Choi, C., Q. (2008). Saving Kermit. Scientific American (2008) Vol. 299 Issue 1, p27-28 Maerz, J., C., Nuzzo, V., A., Blossey, B. (2009). Declines in Woodland Salamander Abundance Associated with Non-Native Earthworm and Plant Invasions. Conservative Biology, Volume 23, No. 4. pp. 975-981 Prince George’s County Department of Parks and Recreation (2013). Watkins Nature Center. PG Parks (2013). Retrieved from http://www.pgparks.com/Things_To_Do/Nature/Watkins_Nature_Center.htm Ronsheim, Dr. Margaret L. (2012). Invasive species and their effects on native species. In AccessScience.McGraw Hill Education. Retrieved from http://www.accessscience.com/content/invasive-species-and-their-effects-on-nativespecies/YB120273 FUTURE RESEARCH As stated in the introduction, there are many factors contributing to the rapid decline of amphibians at Watkins Park, not just a competing earthworm population. In future experiments, we hope to better the experimentation done for pH and nitrogen levels, so the data and results from them will be usable. We also want to test other factors that may be limiting the population at Watkins Park, like direct human human contact and other competitors. In the further future, we hope to examine other ecosystems that amphibians are supposed to be present and determine possible reasons why they are limiting there, then determine if there are common causes in the limitation of these amphibians. Total Quantity of Amphibia and Earthworms Quantity of Amphibia vs. Quantity of Earthworms on Average