Becca Goslow- WMHS
Frosilda Pushani- SHHS
Did You Know…?
http://thescienceofacne.com/amoxicillin/
https://harveymillican.wordpress.com/tag/amoxicillin/
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Watch This!
https://www.youtube.com/watch?v=RpKZvnJwicA
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Terms to know
Antibiotic Resistance
 Escherichia coli
 Ampicillin
 Inhibition Zone

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What is Antibiotic Resistance?
Antibiotics are no longer an option.
 Bacteria takes over and becomes immune.

http://www.medicinenet.com/antibiotic_resistance/page4.htm
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Escherichia coli
http://www.britannica.com/blogs/2011/06/evil-coli/
http://www.health32.com/e-coli-symptoms-and-treatment/
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Ampicillin
www.newlystar-medtech.com/Ampicillin-Capsules-12.html http://www.drugs.com/cdi/ampicillin.html
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Inhibition Zones
http://web.med.unsw.edu.au/cdstest/gtf_cds_site/webpages/plates/plate1a.htm
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Overview
Problem/Hypothesis.
 Materials/Procedure.
 Results/Statistics.
 Accept/Reject Hypothesis.
 Possible Improvements.

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Problem
 The
effect of antibiotics on the growth of
bacteria throughout generations.
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Hypothesis

If Ampicillin is used, then the Escherichia coli
will develop a resistance to the antibiotic.
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Materials
Blank
Disks
Ampicillin
Sterile
Swabs
Petri
Dishes
Analytic
Balance
Starter Plate
(E. coli)
Incubators
Bunsen
Burner
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Values
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Procedure
https://www.youtube.com/watch?v=cneascR3OEc
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Procedure
https://www.youtube.com/watch?v=bRadiLXkqoU
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Procedure
http://en.wikipedia.org/wiki/Capsule_(pharmacy)
http://www.carolina.com/microbiology-supplies/antibiotic-sensitivity-disks-blankGoslow-Pushani
sterile-vial-50/805091.pr
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Procedure
http://www.ufz.de/iwas-sachsen/index.php?en=21853
http://out-grow.com/cultivation-equipment-c-1/
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Procedure
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Procedure
(A+B+C) ÷ 3 = Size
of an inhibition zone.
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Resistance
E. coli.
 Ampicillin.
 Zone of Inhibition/ Resistance.

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Results
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Observations
Date
Observation
3/11/14
One Petri dish had a smaller inhibition zone from the rest. The
E. coli was smelly and white.
3/13/14
The Petri dishes had a lot of E. coli, the bacteria was white and extremely
smelly.
3/14/14
The E. coli on some of the Petri dishes were blotchy.
3/18/14
The E. coli was smellier than usual and filled up every Petri dish completely,
besides the zone of inhibition.
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Effect of Ampicillin
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Effect of Incubation Temperature
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Interaction Effect
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Outliers?
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Standards Plot
12
Size of the Zone of Inhibition
10
8
6
4
2
0
0
1
2
3
4
5
6
7
8
9
10
11
12
Standard Trials
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Recap
What we planned.
 What happened.
 Added Ampicillin to E. coli and incubated
the petri dishes.
 The bacteria and inhibition zones grew.
 Overtime, the inhibitions decreased in size.
 The hypothesis was accepted!

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Improvements for the
Future
Run more trials.
 Have a wider range of the predictor
variables.
 Use of an actual human.
 Stop experimental flaws.

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Flaw and Human Errors
Off temperatures.
 Off measurements of Ampicillin.
 Time issues.

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Who does this effect?
Chemists/Scientists
 Doctors
 YOU!

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Save Your Life
Do not take the antibiotics for viral infections.
 Take an antibiotic exactly as a healthcare
provider tell you.
 Keep antibiotics to a minimum.

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Acknowledgements
Mr. Acre
 Mr. Estapa
 Mrs. Gravel
 Veronica & Abigail

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Works Cited
"Ampicillin Capsules." Ampicillin Capsules. Web. 28 May 2014.
"Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com." Ampicillin Capsules: Indications, Side
Effects, Warnings - Drugs.com. Web. 27 May 2014.
"Antibiotic Resistance." Antibiotic Resistance. University of Utah Health Sciences, n.d. Web. 14 May 2014.
"Antibiotic Resistance a Serious Threat." The Australian Government, n.d. Web. 10 May 2014.
"Antibiotic Resistance Questions & Answers." Centers for Disease Control and Prevention. Centers for Disease
Control and Prevention, 18 Dec. 2013. Web. 10 May 2014.
"Antibiotic Sensitivity Disks, Blank, Sterile, Vial 50." Science Supplies & Curriculum. Web. 28 May 2014.
"Aseptic Technique." YouTube. YouTube. Web. 28 May 2014.
"Capsule (pharmacy)." Wikipedia. Wikimedia Foundation, 24 May 2014. Web. 28 May 2014.
"Cultivation Equipment." : Out-Grow, Mushroom Growing Supplies & Quality Service. Web. 28 May 2014.
Dzidic, Senka. "Antibiotic Resistance Mechanisms in Bacteria: Biochemical and Genetic Aspects." Food Technology
and Biotechnology. N.p., n.d. Web. 15 Mar. 2014.
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Works Cited
"E.coli Symptoms and Treatment." Health32Com Ecoli Symptoms and Treatment Comments.Web. 28 May 2014.
"Evil E. Coli." Encyclopedia Britannica Online. Encyclopedia Britannica. Web. 28 May 2014.
"HowStuffWorks.” "How Do Antibiotics Work?"" HowStuffWorks. N.p., n.d. Web. 26 May 2014.
"International Water Research Alliance Saxony." Running Waters, Microbiology -. Web. 28 May 2014.
"Media Prep." YouTube. YouTube. Web. 28 May 2014.
Paphitou, Niki I. "Antimicrobial Resistance: Action to Combat the Rising Microbial Challenges.” International Journal
of
Antimicrobial Agents. N.p., n.d. Web. 15 Mar. 2014.
"Plate1A." Plate1A. Web. 27 May 2014.
"Science Fair Projects - Bacteria resistance to antibiotics." Science Fair Projects - Bacteria resistance to antibiotics. All
Science Fair Projects, n.d. Web. 14 May 2014.
"The Science of Acne." The Science of Acne. Web. 28 May 2014.
"Tag Archives: Amoxicillin." Harvey Millican Raising Your Kids Without Lowering Your IQ. Web. 28 May 2014.
"The Threat of Antibiotic Resistance." YouTube. YouTube. Web. 28 May 2014.
Todar, Kenneth . "Bacterial Resistance to Antibiotics." Bacterial Resistance to Antibiotics. N.p., n.d. Web. 14 May 2014.
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Any Questions?
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