Becca Goslow- WMHS Frosilda Pushani- SHHS Did You Know…? http://thescienceofacne.com/amoxicillin/ https://harveymillican.wordpress.com/tag/amoxicillin/ Goslow-Pushani 2 Watch This! https://www.youtube.com/watch?v=RpKZvnJwicA Goslow-Pushani 3 Terms to know Antibiotic Resistance Escherichia coli Ampicillin Inhibition Zone Goslow-Pushani 4 What is Antibiotic Resistance? Antibiotics are no longer an option. Bacteria takes over and becomes immune. http://www.medicinenet.com/antibiotic_resistance/page4.htm Goslow-Pushani 5 Escherichia coli http://www.britannica.com/blogs/2011/06/evil-coli/ http://www.health32.com/e-coli-symptoms-and-treatment/ Goslow-Pushani 6 Ampicillin www.newlystar-medtech.com/Ampicillin-Capsules-12.html http://www.drugs.com/cdi/ampicillin.html Goslow-Pushani 7 Inhibition Zones http://web.med.unsw.edu.au/cdstest/gtf_cds_site/webpages/plates/plate1a.htm Goslow-Pushani 8 Overview Problem/Hypothesis. Materials/Procedure. Results/Statistics. Accept/Reject Hypothesis. Possible Improvements. Goslow-Pushani 9 Problem The effect of antibiotics on the growth of bacteria throughout generations. Goslow-Pushani 10 Hypothesis If Ampicillin is used, then the Escherichia coli will develop a resistance to the antibiotic. Goslow-Pushani 11 Materials Blank Disks Ampicillin Sterile Swabs Petri Dishes Analytic Balance Starter Plate (E. coli) Incubators Bunsen Burner Goslow-Pushani 12 Values Goslow-Pushani 13 Procedure https://www.youtube.com/watch?v=cneascR3OEc Goslow-Pushani 14 Procedure https://www.youtube.com/watch?v=bRadiLXkqoU Goslow-Pushani 15 Procedure http://en.wikipedia.org/wiki/Capsule_(pharmacy) http://www.carolina.com/microbiology-supplies/antibiotic-sensitivity-disks-blankGoslow-Pushani sterile-vial-50/805091.pr 16 Procedure http://www.ufz.de/iwas-sachsen/index.php?en=21853 http://out-grow.com/cultivation-equipment-c-1/ Goslow-Pushani 17 Procedure Goslow-Pushani 18 Procedure (A+B+C) ÷ 3 = Size of an inhibition zone. Goslow-Pushani 19 Resistance E. coli. Ampicillin. Zone of Inhibition/ Resistance. Goslow-Pushani 20 Results Goslow-Pushani 21 Observations Date Observation 3/11/14 One Petri dish had a smaller inhibition zone from the rest. The E. coli was smelly and white. 3/13/14 The Petri dishes had a lot of E. coli, the bacteria was white and extremely smelly. 3/14/14 The E. coli on some of the Petri dishes were blotchy. 3/18/14 The E. coli was smellier than usual and filled up every Petri dish completely, besides the zone of inhibition. Goslow-Pushani 22 Effect of Ampicillin Goslow-Pushani 23 Effect of Incubation Temperature Goslow-Pushani 24 Interaction Effect Goslow-Pushani 25 Outliers? Goslow-Pushani 26 Goslow-Pushani 27 Standards Plot 12 Size of the Zone of Inhibition 10 8 6 4 2 0 0 1 2 3 4 5 6 7 8 9 10 11 12 Standard Trials Goslow-Pushani 28 Recap What we planned. What happened. Added Ampicillin to E. coli and incubated the petri dishes. The bacteria and inhibition zones grew. Overtime, the inhibitions decreased in size. The hypothesis was accepted! Goslow-Pushani 29 Improvements for the Future Run more trials. Have a wider range of the predictor variables. Use of an actual human. Stop experimental flaws. Goslow-Pushani 30 Flaw and Human Errors Off temperatures. Off measurements of Ampicillin. Time issues. Goslow-Pushani 31 Who does this effect? Chemists/Scientists Doctors YOU! Goslow-Pushani 32 Save Your Life Do not take the antibiotics for viral infections. Take an antibiotic exactly as a healthcare provider tell you. Keep antibiotics to a minimum. Goslow-Pushani 33 Acknowledgements Mr. Acre Mr. Estapa Mrs. Gravel Veronica & Abigail Goslow-Pushani 34 Works Cited "Ampicillin Capsules." Ampicillin Capsules. Web. 28 May 2014. "Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com." Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com. Web. 27 May 2014. "Antibiotic Resistance." Antibiotic Resistance. University of Utah Health Sciences, n.d. Web. 14 May 2014. "Antibiotic Resistance a Serious Threat." The Australian Government, n.d. Web. 10 May 2014. "Antibiotic Resistance Questions & Answers." Centers for Disease Control and Prevention. Centers for Disease Control and Prevention, 18 Dec. 2013. Web. 10 May 2014. "Antibiotic Sensitivity Disks, Blank, Sterile, Vial 50." Science Supplies & Curriculum. Web. 28 May 2014. "Aseptic Technique." YouTube. YouTube. Web. 28 May 2014. "Capsule (pharmacy)." Wikipedia. Wikimedia Foundation, 24 May 2014. Web. 28 May 2014. "Cultivation Equipment." : Out-Grow, Mushroom Growing Supplies & Quality Service. Web. 28 May 2014. Dzidic, Senka. "Antibiotic Resistance Mechanisms in Bacteria: Biochemical and Genetic Aspects." Food Technology and Biotechnology. N.p., n.d. Web. 15 Mar. 2014. Goslow-Pushani 35 Works Cited "E.coli Symptoms and Treatment." Health32Com Ecoli Symptoms and Treatment Comments.Web. 28 May 2014. "Evil E. Coli." Encyclopedia Britannica Online. Encyclopedia Britannica. Web. 28 May 2014. "HowStuffWorks.” "How Do Antibiotics Work?"" HowStuffWorks. N.p., n.d. Web. 26 May 2014. "International Water Research Alliance Saxony." Running Waters, Microbiology -. Web. 28 May 2014. "Media Prep." YouTube. YouTube. Web. 28 May 2014. Paphitou, Niki I. "Antimicrobial Resistance: Action to Combat the Rising Microbial Challenges.” International Journal of Antimicrobial Agents. N.p., n.d. Web. 15 Mar. 2014. "Plate1A." Plate1A. Web. 27 May 2014. "Science Fair Projects - Bacteria resistance to antibiotics." Science Fair Projects - Bacteria resistance to antibiotics. All Science Fair Projects, n.d. Web. 14 May 2014. "The Science of Acne." The Science of Acne. Web. 28 May 2014. "Tag Archives: Amoxicillin." Harvey Millican Raising Your Kids Without Lowering Your IQ. Web. 28 May 2014. "The Threat of Antibiotic Resistance." YouTube. YouTube. Web. 28 May 2014. Todar, Kenneth . "Bacterial Resistance to Antibiotics." Bacterial Resistance to Antibiotics. N.p., n.d. Web. 14 May 2014. Goslow-Pushani 36 Any Questions? Goslow-Pushani 37 Goslow-Pushani 38