The Effect of Resveratrol on Fat
Storage in C. elegans
By Chris LoBello
Obesity
http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps
Adipocytes
http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt
http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg
PPARg
http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html
Chung and Kurtev- Sirt1 promotes fat mobilization in white
adipocytes by repressing PPAR-g. Nature (2004)
http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf
Wood and Sinclair- Sirtuin activators mimic
caloric restriction and
delay ageing in metazoans. Nature (2004)
Fei and Liu- Relevance of NAC-2, an Na+
coupled citrate transporter, to life
span, body size and fat content in
Caenorhabditis elegans. Biochemical
Society (2004)
http://www.nature.com/nature/journal/v430/n7000/fig_ta
b/nature02789_F2.html
http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044
&blobtype=pdf
Methodology
The Effect of Resveratrol on Fat Storage in C. elegans
Control Group: 0
microliters of
resveratrol
N= N2 Wild Type
Strain Culture
Nile Red staining
solution
(.1microgram/ml)
Done up to days 6 to
8 in the protocol
Group 1: 1
microliter
of
resveratrol
N= N2
Wild Type
Strain
Culture
Group 2: 10
microliters
of
resveratrol
N= N2 Wild
Type Strain
Culture
Group 3: 50
microliters
of
resveratrol
N= N2 Wild
Type Strain
Culture
Group 4:
100
microliters
of
resveratrol
N= N2 Wild
Type Strain
Culture
Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol
Fluorescent intensity determined by using images taken from microscope to place in
Photoshop to determine the strength of the fluorescence
Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test
Budget
Vendor
Order #
Quantity
Description
Price
Total Price
Carolina
173525
1
C. elegans culture kit
$73.00 $73.00
Sigma-Aldrich
N3013-100MG
1
100 MG Nile Red
$64.20 $64.20
Staining
Sigma-Aldrich
R5010-100MG
1
100 MG Resveratrol
$81.50 $81.50
Science
-
1
Photoshop program
-
Research
-
Do-Ability
• C. elegans can be used as a valuable model
organism for the study
• Nile Red Staining will successfully help
examine resveratrol’s effect on the C. elegans
• Results of the staining can be quantified by
using Photoshop
Purpose
To determine the effects of varying amounts
of resveratrol on the fat content of C. elegans
Hypothesis
Null Hypothesis: There will be equal amounts of fat content in
cells with resveratrol supplemented and without resveratrol
supplemented
Alternate Hypothesis: There will be less fat content in the cells
supplemented with resveratrol, and that there will be an
inverse relationship between resveratrol supplementation and
fat content
Bibliography
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