Gregor Mendel’s monastery garden. Fig. 2.2 1 Removed stamens from purple flower 2 Transferred sperm- bearing pollen from stamens of white flower to eggbearing carpel of purple flower Parental generation (P) 3 Pollinated carpel Stamens Carpel (male) (female) matured into pod 4 Planted seeds from pod 5 Examined First generation offspring (F1) offspring: all purple flowers Dominant Recessive X X X X X X EXPERIMENT True-breeding purple-flowered pea plants and white-flowered pea plants were crossed (symbolized by ). The resulting F1 hybrids were allowed to self-pollinate or were crosspollinated with other F1 hybrids. Flower color was then observed in the F2 generation. P Generation (true-breeding parents) Purple flowers White flowers F1 Generation (hybrids) All plants had purple flowers RESULTS Both purple-flowered plants and whiteflowered plants appeared in the F2 generation. In Mendel’s experiment, 705 plants had purple flowers, and 224 had white flowers, a ratio of about 3 purple : 1 white. F2 Generation White (pp) Purple (Pp) Gametes p Purple (PP) p P Gametes Gametes Purple (Pp) P p PP Pp P Pp Pp Gametes p P Pp Pp F1 generation All purple Pp pp F2 generation ¾ purple, ¼ white Parental P0 cross P P p p F1 cross P P p Determine the genotype and phenotype p Parental P0 cross p p P Pp Pp P Pp Pp F1 cross P p P PP Pp Determine the genotype and phenotype p Pp pp Phenotype Purple 3 Purple Genotype PP (homozygous) 1 Pp (heterozygous) 2 Pp (heterozygous) Purple 1 Figure 14.6 White pp (homozygous) Ratio 3:1 Ratio 1:2:1 1 copyright cmassengale 17 RrYy RY Ry rY ry x RrYy RY Ry rY ry All possible gamete combinations copyright cmassengale 18 RY Ry rY ry RY Ry rY ry copyright cmassengale 19 RY RY RRYY Ry rY ry RRYy RrYY RrYy Ry rY ry RRYy RrYY RrYy RRyy RrYy Rryy RrYy rrYY rrYy Rryy rrYy rryy copyright cmassengale Round/Yellow: Round/green: 9 3 wrinkled/Yellow: 3 wrinkled/green: 1 9:3:3:1 phenotypic 20 ratio LAW DOMINANCE SEGREGATION INDEPENDENT ASSORTMENT PARENT CROSS OFFSPRING TT x tt tall x short 100% Tt tall Tt x Tt tall x tall 75% tall 25% short RrGg x RrGg round & green x round & green 9/16 pods 3/16 pods 3/16 pods 1/16 pods copyright cmassengale round seeds & green round seeds & yellow wrinkled seeds & green wrinkled seeds & yellow 21 copyright cmassengale 22 r r R R copyright cmassengale 23 r r R Rr Rr R Rr Rr produces the F1 generation All Rr = pink (heterozygous pink) copyright cmassengale 24 copyright cmassengale 25 copyright cmassengale 26 IA i IB IAIB IBi IB IAIB IBi copyright cmassengale 1/2 = IAIB 1/2 = IBi 27 • Example: male Type O (ii) x female type AB (IAIB) IA IB i IAi IBi i IAi IBi copyright cmassengale 1/2 = IAi 1/2 = IBi 28 copyright cmassengale 29 Example: Eye color in fruit flies Sex Chromosomes fruit fly eye color XX chromosome - female Xy chromosome - male copyright cmassengale 30 Xr Xr XR Y copyright cmassengale 31 Xr XR XR Xr Y Xr Y Xr XR Xr Xr Y 50% red eyed female 50% white eyed male copyright cmassengale 32 copyright cmassengale 33 Genotypes could be: SS or Ss for stars What would the genotype for no stars have to be? R r R RR Rr r Rr rr R r R RR Rr R RR Rr