Cell Membrane and Transport HOW THE CELL ABSORBS AND EXCRETES VARIOUS MOLECULES Cell Membrane Cell membrane separates the components of a cell from its environment (what surrounds the cell) and controls the passage of materials into and out of a cell. Composed of a phospholipid bilayer (75-95%), and proteins (5-25%). Hydrophilic headsAttracted to water Hydrophobic tailsRepelled from water Membrane Proteins Membrane proteins are used for Communication I.D. Tag Anchors Gates Pumps Cell Membrane Structure Fluid Mosaic Model- molecules and proteins are “free floating” along the phospholipid bilayer. Fluid Mosaic Model 3 Types of Proteins Found in Cell Membrane Channel Proteins- allow molecules or ions to enter the cell. They can be open or gated. Receptor Proteins- relay information from outside the cell to inside the cell. Marker Proteins- act as the name tag of the cell. Factors Affecting Transport Molecules size and charge The cell membrane is permeable to small neutral molecules, H2O, gases, and lipids. The cell membrane is impermeable to charged molecules. Proteins are needed to move these across the membrane. Concentration gradient Molecules like to move from areas of high concentration to areas of low concentration Electrical gradient The inner membrane of the cell is slightly negative (-) to the outer surface of the cell. Therefor (+) charged molecules will be attracted into the cell. Concentration Gradient- the difference in concentration across a space. Passive Transport High Low • “Down” concentration gradient. No energy needed. Active Transport Low High • “Against” concentration gradient. Requires energy. Moving Down the Concentration Gradient Moving molecules down their concentration gradient is known as Passive Transport. The cell uses no energy to move the molecules. Processes that used passive transport are… Diffusion Osmosis Facilitated Diffusion Diffusion Diffusion is the movement of molecules from an area of higher concentration to an area of lower concentration. When the concentration of molecules is the same throughout the space it occupies they are in a state of Equilibrium. No energy needed Osmosis Osmosis is the process by which water molecules diffuse across a cell membrane from an area of higher concentration to an area of lower concentration. The direction of movement of water molecules depends on the relative concentration of solutes on the two sides of the semi-permeable membrane. There are three osmolality's ◦ Hypotonic ◦ Hypertonic ◦ Isotonic Hypotonic Solutions When the concentration of solute molecules outside the cell is lower than the concentration inside the cell, the solution outside the cell is hypotonic to the cytosol. Water molecules will have a net movement into the cell causing the cell to swell. The force that water molecules exert against cell wall is called osmotic pressure. If a cell takes in to much water it can burst. This is termed cytolysis. the Hypotonic Solution Plant cell Animal Cell Hypertonic Solutions When the concentration of solute molecules outside the cell is higher than the concentration in the cytosol, the solution outside is hypertonic. Water will have a net movement out of the cell causing the cell to shrivel. Plasmolysis is when osmotic pressure is lost due to water leaving the cell. This is what causes plants to wilt. Hypertonic Solutions Plant Cell Animal Cell Isotonic When the concentration of solutes outside and inside the cell are equal, the outside solution is said to be isotonic to the cytosol. There will be no net movement. Isotonic Solutions Plant Cell Animal Cell What will happen to the cell? 10% Solute 90% Water 5% Solute 95% Water What will happen to the cell? 10% Solute 90% Water 10% Solute 90% Water What will happen to the cell? 10% Solute 90% Water 30% Solute 70% Water Facilitated Diffusion This type of transport is for molecules that may not be soluble or are to large to pass through the membrane pores. Requires protein channels or carrier proteins which move the molecules down their concentration gradient. Protein channels are specific to one type of molecule Some are always open, others are gated and require a trigger to open and close. No energy needed