AP Biology Discussion Notes Thursday 01/22/2015 Goals for Today • Be able to describe how restriction enzymes work and why they are valuable to scientists • Be able to describe & demonstrate gel electrophoresis. • Be able to stop smugglers and other bad guys using restriction enzymes! Question of the Day 1/22 • Why can we use electricity to move DNA through the gel in gel electrophoresis? • What are the charges at the beginning and end of the gels? • Gel Runs • ___________ ___________ Question of the Day 1/22 • Why can we use electricity to move DNA through the gel in gel electrophoresis? • What are the charges at the beginning and end of the gels? • Gel Runs • ___________ ___________ Work Order for Today Stop smugglers! 1. Analyze your results with gel electrophoresis (blue sheet). 2. Finish analysis Questions to help future scientists stop smugglers! 3. Work on Species of the Day (Eagle) sheet from yesterday 4. Study for DNA structure/replication/ names quiz tomorrow 5. Nobel Prize reading Work Order for Today Stop smugglers! 1. Analyze your results with gel electrophoresis (blue sheet). 2. Finish analysis Questions to help future scientists stop smugglers! 3. Work on Species of the Day (Eagle) sheet from yesterday 4. Study for DNA structure/replication/ names quiz tomorrow 5. Nobel Prize reading Species of the Day 1/21 • Bald Eagle – Haliaeetus leucocephalus If I have a DNA sequence from a Leucistic eagle that appears to only differ at one base pair sequence from other traditionally colored eagles, how could I test to see if this was true for other traditionally colored eagles & Leucistic eagles without sequencing their DNA? Explain how you would do this! DNA Assignment • Not a judgment of you or your ability! • Not busy work! – Help you to succeed in AP Biology, on the AP test, and in life • Make sure you understand what you are writing DNA Assignment common mistakes • Not answering the WHOLE question! – Ex. #6,11,15,20 • Not answering the question that was asked! – Ex. #7,11 • Not showing work – FREE POINTS • NOT READING CAREFULLY • NOT thinking/answering/describing carefully QUIZ TOMORROW • DNA Structure, DNA Replication, & Names!