Transcription Transcription uses the information in a single gene in the DNA to make a messenger RNA (mRNA) molecule. DNA is a polymer of four types of nucleotides, G, C, A and T, and RNA is a polymer of four corresponding types of nucleotides, G, C, A and U (Uracil instead of Thymine!). During transcription, the enzyme, RNA polymerase, separates the two strands of DNA and makes the mRNA molecule by adding RNA nucleotides, one at a time. Each RNA nucleotide is joined to the previous nucleotide by a covalent bond. Each DNA nucleotide in the gene is matched by a complementary RNA nucleotide which has a matching shape and charge distribution. The base-pairing rules summarize which pairs of the strands are complimentary. The base-pairing rules for transcription are very similar to the base-pairing rules in the DNA double helix. The basepairing rules ensure that the message from the nucleotide sequence in the gene in the DNA is copied into a corresponding nucleotide sequence in the mRNA molecule. DNA replication Transcription The whole chromosome is replicated. Just one gene is transcribed. DNA is made. ________ is made. DNA is double-stranded. mRNA is _________-stranded. DNA polymerase is the enzyme which carries out DNA replication. ________ _______ is the enzyme which carries out transcription. T = thymine is used in DNA, T = thymine is replaced by ____ = ________ in RNA, so A pairs with T in DNA. so A in DNA pairs with ___ in mRNA.