9.15.14 - Iowa State University

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Intro to Replication

Supplemental Instruction

Iowa State University

Leader: Lilli Howard

Course: BIOL/GEN 313

Instructor: Dr. Rodermel/Dr. Tuggle

Date: 09/03/14

1.

REVIEW: Draw and name the stages of mitosis.

2.

Replication proceeds _______ to ______.

3.

DNA is replicated by the process of ________________________________ replication. a. Describe in your own words this process. (Use a diagram if it helps) b. Describe the experiment that proved this concept.

4.

In Meselson and Stahl’s experiment, what would the results look like if DNA replication were: a.

Conservative b.

Dispersive

1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu

5.

How many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?

6.

Discontinuous replication is a result of which property of DNA?

a.

Complementary bases b.

Charged phosphate group c.

Antiparallel nucleotide strands d.

Five carbon sugar

7.

Which type of replication requires a break in the nucleotide strand to get started?

a.

Theta replication b.

Rolling circle replication c.

Linear eukaryotic replication d.

All of the above

8.

A _____________ is the unit of replication; it is length of DNA from one ___________ to the next .

9.

Draw a molecule of DNA undergoing (a) theta replication (b) rolling-circle replication (c) eukaryotic replication. On your drawing identify: a.

origin of replication b.

polarity c.

leading and lagging strands d.

okazaki fragments e.

location of primers

10.

Fill in the following table.

1060 Hixson-Lied Student Success Center  515-294-6624  sistaff@iastate.edu  http://www.si.iastate.edu

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