Who Wants to Be a Millionaire?! Goal: Reach $1,000,000 • 4 teams of 7-8 students This is your “audience” when you use a life-line. • Each person on each team will answer the given question individually. • If you are stuck, you can use a life-line. Each team gets 4 life-lines: • 2 “phone a friend” choose someone on your team to bail you out • 2 “ask the audience” discuss with your entire team the correct answer •Each Q you answer correctly, you earn a level on the board. •If you answer incorrectly, you move down a level on the board. •Goal is to reach $1,000,000 1 - $100 2 - $200 3 - $500 4 - $1,000 5 - $2,000 6 - $4,000 7 - $6,000 8 - $8,000 9- $10,000 10- $20,000 PRIZES 11 - $30,000 12 - $50,000 13 - $75,000 14 - $100,000 15 - $200,000 16 - $350,000 17 - $500,000 18 - $750,000 19 - $900,000 20 - $1,000,000 Ready? What is the term for a nucleotide triplet (3 bases ) on mRNA? CODON DNA or RNA? Contains Uracil RNA DNA or RNA Single stranded RNA DNA or RNA Contains G &C BOTH DNA or RNA Cannot leave nucleus DNA Where does translation take place? RIBOSOME If DNA has 40% A, how much (%) C will it contain? 10% C + 10% G 40% A = 40% T Which enzyme adds new RNA nucleotides to DNA template strand to make mRNA? RNA POLYMERASE What is the first step of protein synthesis? TRANSCRIPTION What is created during translation? AMINO ACIDS (PROTEINS) Term for the copying of DNA? DNA REPLICATION What is the term for spontaneous changes in the DNA sequence? MUTATIONS When DNA is copied, each daughter helix will contain what % of original DNA? 50% ORIGINAL 50 % NEW What is the term for a mutation that results from an insertion of an extra base? FRAMESHIFT How many nucleotides are necessary to code for 2 amino acids? 6 NUCLEOTIDES (2 CODONS) Which enzyme unzips DNA during DNA replication? DNA HELICASE Which enzyme adds new DNA nucleotides to the old DNA template strand? DNA POLYMERASE Which enzyme unzips DNA during protein synthesis? RNA POLYMERASE What type of mutation describes a change from TAC TAG to TAC TA_? FRAMESHIFT (DELETION) What is created during transcription? mRNA What do anti-codons bind to? CODONS ON MRNA What is the sugar found in RNA? RIBOSE Fill in the blank: DNA RNA _______ Trait PROTEIN Do both prokaryotes & eukaryotes carry out translation in the ribosome? YES What is the monomer/subunit of DNA & RNA? NUCLEOTIDE What are the 3 parts of a nucleotide? PHOSPHATE, 5-C SUGAR & A NITROGENOUS BASE What is the sugar found in DNA? DEOXYRIBOSE What is D? mRNA On what molecule are anti-codons found? TRNA What type of bonds connect the sugars and phosphates in DNA? COVALENT BONDS How many bases make up 1 anti-codon? 3 BASES What is the role of tRNA & anti-codons? TO CARRY AMINO ACIDS TO THE RIBOSOME How does the ribosome know which amino acid to call for? READS THE CODON ON MRNA & CALLS FOR MATCHING ANTICODON Where does transcription take place? NUCLEUS If mRNA: AUC UUA DNA: ? DNA: TAG AAT What is the process shown here? TRANSLATION What is the mRNA codon complementary to? tRNA anti-codon What type of bonds hold connect the 2 strands of DNA? HYDROGEN BONDS What are the building blocks of proteins that are connected during translation? AMINO ACIDS Where is rRNA found? IN RIBOSOMES Term for artificially created DNA from 2 different sources? RECOMBINANT DNA What is the term for proteins that DNA wraps around? HISTONES What is B? AMINO ACID What is the term for substances that cause mutations? MUTAGEN What types of cells undergo protein synthesis? ALL CELLS (even prokaryotes) Do prokaryotes carry out transcription in the nucleus? NO (THEY LACK A NUCLEUS) What is the term for DNA that is wrapped around proteins? CHROMOSOME What is a type of mutation where the DNA changes but the resulting amino acid does not? SILENT MUTATION What makes up the backbone of DNA? SUGAR AND PHOSPHATES Are all genes expressed all the time? Explain. No. When they are not needed, they are “turned off”. Do codons code for only 1 amino acid? Can multiple codons code for the same amino acid? MULTIPLE CODONS CODE FOR SAME AMINO ACID What is A? Protein or polypeptide What is C? tRNA What type of bond connects amino acids together? PEPTIDE BOND Term for cutting genes from one organism and inserting into another organism’s DNA? GENE SPLICING Inserting genes into patients to treat diseases (replace faulty disease causing DNA) GENE THERAPY Placing a cloned embryo into organism’s uterus to grow a new individual? REPRODUCTIVE CLONING Growing newly cloned embryo in a petri dish without implanting into a uterus THERAPEUTIC CLONING Running an electric current through DNA samples to determine an individual’s DNA pattern DNA FINGERPRINTING How many DNA molecules are created during DNA replication? 2 IDENTICAL MOLECULES OF DNA What % of 1 DNA molecule created during replication is considered “new” DNA? 50% New 50% old Term for mutation that originally coded for an amino acid which now codes for a STOP codon? NONSENSE MUTATION Term for allowing only organisms with certain traits to reproduce to give desired offspring SELECTIVE BREEDING (ARTIFICIAL SELECTION) How many TOTAL strands are seen at the end of DNA replication? 4 DNA strands What is the term for multiple amino acids together? POLYPEPTIDE What does GMO stand for? GENETICALLY MODIFIED ORGANISM