Name: __________________________________ Date: ____________________ Study Guide (Part II) Campbell Biology Unit 4 Evolution Short Response Questions: (I will choose two of the following short answer prompts for the Unit 4 Exam Part II) 1. Describe how a plasmid can be genetically modified to include a piece of foreign DNA that alters the phenotype of bacterial cells transformed with the modified plasmid. Describe a procedure to determine which bacterial cells have been successfully transformed. 2. What is the importance of reproductive isolation in Evolution? Describe a few methods and examples of maintaining reproductive Isolation for a. Prezygotic Barriers b. Postzygotic Barriers 3. Discuss the importance of each of the following to Darwin’s Theory of Natural Selection: c. Variation among individuals d. Heritability of traits e. Competition for Resources 4. Write a short essay evaluating whether changes to an organisms physical environment are likely to result in evolutionary change. Use examples to support your reasoning. Long Response Questions: (I will choose one of the following long answer prompts for the Unit 4 Exam Part II) 1. Phylogeny is the evolutionary history of a species. (a) The evolution of a species is dependent on changes in the genome of the species. Identify TWO mechanisms of genetic change, and explain how each affects genetic variation. (b) Based on the data in the table below, draw a phylogenetic tree that reflects the evolutionary relationships of the organisms based on the differences in their cytochrome c amino-acid sequences and explain the relationships of the organisms. Based on the data, identify which organism is most closely related to the chicken and explain your choice. (c) Describe TWO types of evidence—other than the comparison of proteins— that can be used to determine the phylogeny of organisms. Discuss one strength of each type of evidence you described. THE NUMBER OF AMINO ACID DIFFERENCES IN CYTOCHROME c AMONG VARIOUS ORGANISMS Horse Horse Donkey Chicken Penguin Snake 0 Donkey Chicken Penguin Snake 1 11 13 21 0 10 12 20 0 3 18 0 17 0 2. Fossils of lobe-finned fishes, which are ancestors of amphibians, are found in rocks that are at least 380 million years old. Fossils of the oldest amphibianlike vertebrate animals with true legs and lungs are found in rocks that are approximately 363 million years old. Three samples of rocks are available that might contain fossils of a transitional species between lobe-finned fishes and amphibians: one rock sample that is 350 million years old, one that is 370 million years old, and one that is 390 million years old. (a) Select the most appropriate sample of rocks in which to search for a transitional species between lobe-finned fishes and amphibians. Justify your selection. (b) Describe TWO pieces of evidence provided by fossils of a transitional species that would support a hypothesis that amphibians evolved from lobefinned fishes. 3. Evolution is one of the unifying themes of biology. Evolution involves change in the frequencies of alleles in a population. For a particular genetic locus in a population, the frequency of the recessive allele (a) is 0.4 and the frequency of the dominant allele (A) is 0.6. (a) What is the frequency of each genotype (AA, Aa, aa) in this population? What is the frequency of the dominant phenotype? (b) How can the Hardy-Weinberg principle of genetic equilibrium be used to determine whether this population is evolving? (c) Identify a particular environmental change and describe how it might alter allelic frequencies in this population. Explain which condition of the Hardy-Weinberg principle would not be met.