Lab Report 2
Which Fruit has more DNA?
Title Page
Name of Lab, Name of Student, Date,
Period, Subject
 Name of Lab:
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Which Fruit has more DNA?
The Rest should be self explanatory
Purpose/Hypothesis
 Started
on a new page……
 Purpose:
 What
 Give
 To
do you think it is?
Some examples?
determine which type of berry has
more DNA .
Purpose/Hypothesis
 Hypothesis:
As
a group you developed this….
Your perdition will work as
well…..
Purpose/Hypothesis
 Strawberries
will have more DNA
per gram then any other berries.
 If I extract the DNA from
strawberries, blackberries,
blueberries, and raspberries then
strawberries will have more DNA
per gram of berry.
Pre-Lab Questions
There
are no Pre-Lab
questions.
Here are some examples.
Materials

Contains a complete list of all the material used in
the lab.
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8 test tubes 2 for each fruit
10 mL of DNA extraction buffer solution per fruit
4 Ziploc bags
Ice cold Isopropyl alcohol about 6 mL per fruit
Paper towels for clean up
Pencil
Paper
Lab apron
Safety glasses
Methods, Procedures
& Safety Issues

Give me example of the procedures your
group use.
Methods, Procedures
& Safety Issues
 Safety
 The
Issues
use of Isopropyl alcohol means
there should be no open flames, and
gloves, safety glasses, and lab
aprons should be worn. The fruit juice
and buffer solution could stain clothes
and should be handled with care.
 Any other concerns you can think of?
Methods, Procedures
& Safety Issues

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Place fruit into a Ziploc TM bag and seal shut.
Squish for a few minutes to completely squash
the fruit.
Add 10 ml DNA Extraction Buffer (soapy salty
water) and squish for a few more minutes. Try
not to make a lot of soap bubbles.
Methods, Procedures
& Safety Issues
Filter through a coffee filter set in a funnel,
and collect the liquid in a clear tube. Do not
squeeze the filter. Collect about 3 ml liquid.
 Add 2 volumes ice cold isopropyl alcohol to
the fruit liquid in the tube. Pour the isopropyl
alcohol carefully down the side of the tube
so that it forms a separate layer on top of the
fruit liquid.

Methods, Procedures
& Safety Issues
Watch for about a minute. What do you see?
You should see a white fluffy cloud at the
interface between the two liquids. That’s
DNA!
 Spin and stir the coffee stirrer or transfer
pipet in the tangle of DNA, wrapping the
DNA around the stirrer.

Methods, Procedures &
Safety Issues
Pull out the stirrer and transfer the DNA to a
piece of saran wrap or clean tube. The fibers
are thousands and millions of DNA strands.
 Rinse your funnel. Put the Ziploc TM bag
and paper towel in the garbage.
 Pre-weigh a test tube and small beaker
 Place the DNA in the test tube and measure
the amount of DNA.
 Repeat for each fruit

Data tables and/or
Graphs
Data Table
Mass of fruit
Mass of DNA
DNA/g
Strawberries
13g
1g
1g/13g
Blueberries
13g
.5g
.5g/13g
Blackberries
13g
2g
2g/13g
Raspberries
13g
.25g
.25g/13g
6
5
4
Mass of Fruit in Grams
Mass of DNA in grams
3
2
1
0
Strawberries Blueberries Blackberries Raseberries
Data tables and/or
Graphs
 Data
Tables should be well organized
and easily understandable. It should
also be properly labeled in order to
improve clarity.
 Graphs should fit the data that it
represents. Should be labeled in a way
to present the information in a clear and
understand able way.
Discussion and Analysis
Contains a detailed and accurate account of
the data.
 What is the data saying… What do you get
from it?
 What happen when you combined the
liquids?
 What is the Data table showing
 Use the Data in the discussion.
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Discussion and Analysis

From analyzing the data we can see that
blueberries have the largest amount of DNA
per gram of fruit. To me this was surprising
because blueberries are so small compared
to strawberries. I thought that the larger fruit
would have more DNA due to extra
chromosomes.
Conclusion

My hypothesis was not supported by the
data because it indicated that blueberries
have more DNA per gram of fruit. Possible
sources of error for this lab is not getting the
amount of fruit equal. Also the amount/level
of fruit liquid was not the same per test tube
possible leading to less DNA.